Job ID = 14520934 SRX = SRX8245447 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26346895 spots for SRR11684658/SRR11684658.sra Written 26346895 spots for SRR11684658/SRR11684658.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:07 26346895 reads; of these: 26346895 (100.00%) were paired; of these: 12832255 (48.70%) aligned concordantly 0 times 12361883 (46.92%) aligned concordantly exactly 1 time 1152757 (4.38%) aligned concordantly >1 times ---- 12832255 pairs aligned concordantly 0 times; of these: 356582 (2.78%) aligned discordantly 1 time ---- 12475673 pairs aligned 0 times concordantly or discordantly; of these: 24951346 mates make up the pairs; of these: 13606707 (54.53%) aligned 0 times 10192466 (40.85%) aligned exactly 1 time 1152173 (4.62%) aligned >1 times 74.18% overall alignment rate Time searching: 00:28:07 Overall time: 00:28:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 356489 / 13869590 = 0.0257 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:03:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:03:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:03:26: 1000000 INFO @ Sat, 15 Jan 2022 21:03:38: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:03:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:03:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:03:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:03:48: 3000000 INFO @ Sat, 15 Jan 2022 21:03:58: 4000000 INFO @ Sat, 15 Jan 2022 21:03:59: 1000000 INFO @ Sat, 15 Jan 2022 21:04:09: 5000000 INFO @ Sat, 15 Jan 2022 21:04:12: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:04:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:04:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:04:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:04:20: 6000000 INFO @ Sat, 15 Jan 2022 21:04:25: 3000000 INFO @ Sat, 15 Jan 2022 21:04:28: 1000000 INFO @ Sat, 15 Jan 2022 21:04:30: 7000000 INFO @ Sat, 15 Jan 2022 21:04:38: 4000000 INFO @ Sat, 15 Jan 2022 21:04:38: 2000000 INFO @ Sat, 15 Jan 2022 21:04:41: 8000000 INFO @ Sat, 15 Jan 2022 21:04:49: 3000000 INFO @ Sat, 15 Jan 2022 21:04:52: 5000000 INFO @ Sat, 15 Jan 2022 21:04:53: 9000000 INFO @ Sat, 15 Jan 2022 21:05:00: 4000000 INFO @ Sat, 15 Jan 2022 21:05:04: 10000000 INFO @ Sat, 15 Jan 2022 21:05:05: 6000000 INFO @ Sat, 15 Jan 2022 21:05:10: 5000000 INFO @ Sat, 15 Jan 2022 21:05:15: 11000000 INFO @ Sat, 15 Jan 2022 21:05:18: 7000000 INFO @ Sat, 15 Jan 2022 21:05:21: 6000000 INFO @ Sat, 15 Jan 2022 21:05:26: 12000000 INFO @ Sat, 15 Jan 2022 21:05:31: 8000000 INFO @ Sat, 15 Jan 2022 21:05:32: 7000000 INFO @ Sat, 15 Jan 2022 21:05:37: 13000000 INFO @ Sat, 15 Jan 2022 21:05:43: 8000000 INFO @ Sat, 15 Jan 2022 21:05:43: 9000000 INFO @ Sat, 15 Jan 2022 21:05:48: 14000000 INFO @ Sat, 15 Jan 2022 21:05:55: 9000000 INFO @ Sat, 15 Jan 2022 21:05:56: 10000000 INFO @ Sat, 15 Jan 2022 21:05:59: 15000000 INFO @ Sat, 15 Jan 2022 21:06:06: 10000000 INFO @ Sat, 15 Jan 2022 21:06:08: 11000000 INFO @ Sat, 15 Jan 2022 21:06:10: 16000000 INFO @ Sat, 15 Jan 2022 21:06:17: 11000000 INFO @ Sat, 15 Jan 2022 21:06:21: 17000000 INFO @ Sat, 15 Jan 2022 21:06:21: 12000000 INFO @ Sat, 15 Jan 2022 21:06:28: 12000000 INFO @ Sat, 15 Jan 2022 21:06:32: 18000000 INFO @ Sat, 15 Jan 2022 21:06:34: 13000000 INFO @ Sat, 15 Jan 2022 21:06:39: 13000000 INFO @ Sat, 15 Jan 2022 21:06:43: 19000000 INFO @ Sat, 15 Jan 2022 21:06:46: 14000000 INFO @ Sat, 15 Jan 2022 21:06:50: 14000000 INFO @ Sat, 15 Jan 2022 21:06:53: 20000000 INFO @ Sat, 15 Jan 2022 21:06:58: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:07:01: 15000000 INFO @ Sat, 15 Jan 2022 21:07:04: 21000000 INFO @ Sat, 15 Jan 2022 21:07:11: 16000000 INFO @ Sat, 15 Jan 2022 21:07:11: 16000000 INFO @ Sat, 15 Jan 2022 21:07:14: 22000000 INFO @ Sat, 15 Jan 2022 21:07:22: 17000000 INFO @ Sat, 15 Jan 2022 21:07:23: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:07:24: 23000000 INFO @ Sat, 15 Jan 2022 21:07:33: 18000000 INFO @ Sat, 15 Jan 2022 21:07:35: 24000000 INFO @ Sat, 15 Jan 2022 21:07:36: 18000000 INFO @ Sat, 15 Jan 2022 21:07:44: 19000000 INFO @ Sat, 15 Jan 2022 21:07:45: 25000000 INFO @ Sat, 15 Jan 2022 21:07:48: 19000000 INFO @ Sat, 15 Jan 2022 21:07:54: 20000000 INFO @ Sat, 15 Jan 2022 21:07:55: 26000000 INFO @ Sat, 15 Jan 2022 21:08:00: 20000000 INFO @ Sat, 15 Jan 2022 21:08:05: 27000000 INFO @ Sat, 15 Jan 2022 21:08:05: 21000000 INFO @ Sat, 15 Jan 2022 21:08:12: 21000000 INFO @ Sat, 15 Jan 2022 21:08:14: 28000000 INFO @ Sat, 15 Jan 2022 21:08:15: 22000000 INFO @ Sat, 15 Jan 2022 21:08:24: 22000000 INFO @ Sat, 15 Jan 2022 21:08:25: 29000000 INFO @ Sat, 15 Jan 2022 21:08:27: 23000000 INFO @ Sat, 15 Jan 2022 21:08:35: 30000000 INFO @ Sat, 15 Jan 2022 21:08:36: 23000000 INFO @ Sat, 15 Jan 2022 21:08:38: 24000000 INFO @ Sat, 15 Jan 2022 21:08:46: 31000000 INFO @ Sat, 15 Jan 2022 21:08:48: 25000000 INFO @ Sat, 15 Jan 2022 21:08:49: 24000000 INFO @ Sat, 15 Jan 2022 21:08:56: 32000000 INFO @ Sat, 15 Jan 2022 21:08:59: 26000000 INFO @ Sat, 15 Jan 2022 21:09:01: 25000000 INFO @ Sat, 15 Jan 2022 21:09:06: 33000000 INFO @ Sat, 15 Jan 2022 21:09:09: 27000000 INFO @ Sat, 15 Jan 2022 21:09:14: 26000000 INFO @ Sat, 15 Jan 2022 21:09:16: 34000000 INFO @ Sat, 15 Jan 2022 21:09:19: 28000000 INFO @ Sat, 15 Jan 2022 21:09:26: 35000000 INFO @ Sat, 15 Jan 2022 21:09:26: 27000000 INFO @ Sat, 15 Jan 2022 21:09:30: 29000000 INFO @ Sat, 15 Jan 2022 21:09:36: 36000000 INFO @ Sat, 15 Jan 2022 21:09:39: 28000000 INFO @ Sat, 15 Jan 2022 21:09:40: 30000000 INFO @ Sat, 15 Jan 2022 21:09:46: 37000000 INFO @ Sat, 15 Jan 2022 21:09:51: 29000000 INFO @ Sat, 15 Jan 2022 21:09:51: 31000000 INFO @ Sat, 15 Jan 2022 21:09:56: 38000000 INFO @ Sat, 15 Jan 2022 21:09:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:09:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:09:59: #1 total tags in treatment: 13161244 INFO @ Sat, 15 Jan 2022 21:09:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:09:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:00: #1 tags after filtering in treatment: 9280529 INFO @ Sat, 15 Jan 2022 21:10:00: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:10:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:10:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:02: 32000000 INFO @ Sat, 15 Jan 2022 21:10:03: 30000000 INFO @ Sat, 15 Jan 2022 21:10:12: 33000000 INFO @ Sat, 15 Jan 2022 21:10:15: 31000000 INFO @ Sat, 15 Jan 2022 21:10:23: 34000000 INFO @ Sat, 15 Jan 2022 21:10:27: 32000000 INFO @ Sat, 15 Jan 2022 21:10:32: 35000000 INFO @ Sat, 15 Jan 2022 21:10:38: 33000000 INFO @ Sat, 15 Jan 2022 21:10:42: 36000000 INFO @ Sat, 15 Jan 2022 21:10:50: 34000000 INFO @ Sat, 15 Jan 2022 21:10:52: 37000000 INFO @ Sat, 15 Jan 2022 21:11:01: 38000000 INFO @ Sat, 15 Jan 2022 21:11:02: 35000000 INFO @ Sat, 15 Jan 2022 21:11:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:11:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:11:05: #1 total tags in treatment: 13161244 INFO @ Sat, 15 Jan 2022 21:11:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:05: #1 tags after filtering in treatment: 9280529 INFO @ Sat, 15 Jan 2022 21:11:05: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:11:05: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:14: 36000000 INFO @ Sat, 15 Jan 2022 21:11:25: 37000000 INFO @ Sat, 15 Jan 2022 21:11:36: 38000000 INFO @ Sat, 15 Jan 2022 21:11:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:11:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:11:40: #1 total tags in treatment: 13161244 INFO @ Sat, 15 Jan 2022 21:11:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:41: #1 tags after filtering in treatment: 9280529 INFO @ Sat, 15 Jan 2022 21:11:41: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:11:41: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245447/SRX8245447.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling