Job ID = 14520855 SRX = SRX8245424 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15038506 spots for SRR11684635/SRR11684635.sra Written 15038506 spots for SRR11684635/SRR11684635.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:24 15038506 reads; of these: 15038506 (100.00%) were paired; of these: 6394870 (42.52%) aligned concordantly 0 times 7651121 (50.88%) aligned concordantly exactly 1 time 992515 (6.60%) aligned concordantly >1 times ---- 6394870 pairs aligned concordantly 0 times; of these: 250261 (3.91%) aligned discordantly 1 time ---- 6144609 pairs aligned 0 times concordantly or discordantly; of these: 12289218 mates make up the pairs; of these: 7417669 (60.36%) aligned 0 times 4208398 (34.24%) aligned exactly 1 time 663151 (5.40%) aligned >1 times 75.34% overall alignment rate Time searching: 00:12:24 Overall time: 00:12:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 186317 / 8892696 = 0.0210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:46: 1000000 INFO @ Sat, 15 Jan 2022 20:22:53: 2000000 INFO @ Sat, 15 Jan 2022 20:23:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:08: 4000000 INFO @ Sat, 15 Jan 2022 20:23:16: 1000000 INFO @ Sat, 15 Jan 2022 20:23:16: 5000000 INFO @ Sat, 15 Jan 2022 20:23:23: 6000000 INFO @ Sat, 15 Jan 2022 20:23:23: 2000000 INFO @ Sat, 15 Jan 2022 20:23:30: 7000000 INFO @ Sat, 15 Jan 2022 20:23:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:37: 8000000 INFO @ Sat, 15 Jan 2022 20:23:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:38: 4000000 INFO @ Sat, 15 Jan 2022 20:23:45: 9000000 INFO @ Sat, 15 Jan 2022 20:23:46: 1000000 INFO @ Sat, 15 Jan 2022 20:23:47: 5000000 INFO @ Sat, 15 Jan 2022 20:23:52: 10000000 INFO @ Sat, 15 Jan 2022 20:23:55: 2000000 INFO @ Sat, 15 Jan 2022 20:23:55: 6000000 INFO @ Sat, 15 Jan 2022 20:24:00: 11000000 INFO @ Sat, 15 Jan 2022 20:24:03: 3000000 INFO @ Sat, 15 Jan 2022 20:24:03: 7000000 INFO @ Sat, 15 Jan 2022 20:24:07: 12000000 INFO @ Sat, 15 Jan 2022 20:24:10: 4000000 INFO @ Sat, 15 Jan 2022 20:24:10: 8000000 INFO @ Sat, 15 Jan 2022 20:24:15: 13000000 INFO @ Sat, 15 Jan 2022 20:24:18: 9000000 INFO @ Sat, 15 Jan 2022 20:24:18: 5000000 INFO @ Sat, 15 Jan 2022 20:24:22: 14000000 INFO @ Sat, 15 Jan 2022 20:24:26: 10000000 INFO @ Sat, 15 Jan 2022 20:24:26: 6000000 INFO @ Sat, 15 Jan 2022 20:24:30: 15000000 INFO @ Sat, 15 Jan 2022 20:24:34: 11000000 INFO @ Sat, 15 Jan 2022 20:24:34: 7000000 INFO @ Sat, 15 Jan 2022 20:24:37: 16000000 INFO @ Sat, 15 Jan 2022 20:24:42: 12000000 INFO @ Sat, 15 Jan 2022 20:24:42: 8000000 INFO @ Sat, 15 Jan 2022 20:24:44: 17000000 INFO @ Sat, 15 Jan 2022 20:24:50: 9000000 INFO @ Sat, 15 Jan 2022 20:24:50: 13000000 INFO @ Sat, 15 Jan 2022 20:24:52: 18000000 INFO @ Sat, 15 Jan 2022 20:24:58: 10000000 INFO @ Sat, 15 Jan 2022 20:24:59: 14000000 INFO @ Sat, 15 Jan 2022 20:25:00: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:25:06: 11000000 INFO @ Sat, 15 Jan 2022 20:25:07: 15000000 INFO @ Sat, 15 Jan 2022 20:25:08: 20000000 INFO @ Sat, 15 Jan 2022 20:25:14: 12000000 INFO @ Sat, 15 Jan 2022 20:25:14: 16000000 INFO @ Sat, 15 Jan 2022 20:25:16: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:25:22: 17000000 INFO @ Sat, 15 Jan 2022 20:25:23: 13000000 INFO @ Sat, 15 Jan 2022 20:25:23: 22000000 INFO @ Sat, 15 Jan 2022 20:25:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:25:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:25:25: #1 total tags in treatment: 8458616 INFO @ Sat, 15 Jan 2022 20:25:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:25: #1 tags after filtering in treatment: 6481348 INFO @ Sat, 15 Jan 2022 20:25:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:25:25: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:30: 18000000 INFO @ Sat, 15 Jan 2022 20:25:30: 14000000 INFO @ Sat, 15 Jan 2022 20:25:37: 19000000 INFO @ Sat, 15 Jan 2022 20:25:38: 15000000 INFO @ Sat, 15 Jan 2022 20:25:45: 20000000 INFO @ Sat, 15 Jan 2022 20:25:46: 16000000 INFO @ Sat, 15 Jan 2022 20:25:53: 21000000 INFO @ Sat, 15 Jan 2022 20:25:54: 17000000 INFO @ Sat, 15 Jan 2022 20:26:01: 22000000 INFO @ Sat, 15 Jan 2022 20:26:03: 18000000 INFO @ Sat, 15 Jan 2022 20:26:04: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:04: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:04: #1 total tags in treatment: 8458616 INFO @ Sat, 15 Jan 2022 20:26:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:04: #1 tags after filtering in treatment: 6481348 INFO @ Sat, 15 Jan 2022 20:26:04: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:26:04: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:10: 19000000 INFO @ Sat, 15 Jan 2022 20:26:18: 20000000 INFO @ Sat, 15 Jan 2022 20:26:26: 21000000 INFO @ Sat, 15 Jan 2022 20:26:33: 22000000 INFO @ Sat, 15 Jan 2022 20:26:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:35: #1 total tags in treatment: 8458616 INFO @ Sat, 15 Jan 2022 20:26:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:36: #1 tags after filtering in treatment: 6481348 INFO @ Sat, 15 Jan 2022 20:26:36: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:26:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245424/SRX8245424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling