Job ID = 14520820 SRX = SRX8245417 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9834782 spots for SRR11684628/SRR11684628.sra Written 9834782 spots for SRR11684628/SRR11684628.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:22 9834782 reads; of these: 9834782 (100.00%) were paired; of these: 4583123 (46.60%) aligned concordantly 0 times 4629302 (47.07%) aligned concordantly exactly 1 time 622357 (6.33%) aligned concordantly >1 times ---- 4583123 pairs aligned concordantly 0 times; of these: 39021 (0.85%) aligned discordantly 1 time ---- 4544102 pairs aligned 0 times concordantly or discordantly; of these: 9088204 mates make up the pairs; of these: 4911151 (54.04%) aligned 0 times 3654877 (40.22%) aligned exactly 1 time 522176 (5.75%) aligned >1 times 75.03% overall alignment rate Time searching: 00:09:22 Overall time: 00:09:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 53205 / 5289695 = 0.0101 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:05: 1000000 INFO @ Sat, 15 Jan 2022 20:15:12: 2000000 INFO @ Sat, 15 Jan 2022 20:15:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:25: 4000000 INFO @ Sat, 15 Jan 2022 20:15:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:32: 5000000 INFO @ Sat, 15 Jan 2022 20:15:35: 1000000 INFO @ Sat, 15 Jan 2022 20:15:39: 6000000 INFO @ Sat, 15 Jan 2022 20:15:44: 2000000 INFO @ Sat, 15 Jan 2022 20:15:45: 7000000 INFO @ Sat, 15 Jan 2022 20:15:52: 8000000 INFO @ Sat, 15 Jan 2022 20:15:53: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:59: 9000000 INFO @ Sat, 15 Jan 2022 20:16:02: 4000000 INFO @ Sat, 15 Jan 2022 20:16:06: 1000000 INFO @ Sat, 15 Jan 2022 20:16:06: 10000000 INFO @ Sat, 15 Jan 2022 20:16:12: 5000000 INFO @ Sat, 15 Jan 2022 20:16:13: 2000000 INFO @ Sat, 15 Jan 2022 20:16:13: 11000000 INFO @ Sat, 15 Jan 2022 20:16:20: 12000000 INFO @ Sat, 15 Jan 2022 20:16:20: 6000000 INFO @ Sat, 15 Jan 2022 20:16:20: 3000000 INFO @ Sat, 15 Jan 2022 20:16:27: 13000000 INFO @ Sat, 15 Jan 2022 20:16:28: 4000000 INFO @ Sat, 15 Jan 2022 20:16:29: 7000000 INFO @ Sat, 15 Jan 2022 20:16:34: 14000000 INFO @ Sat, 15 Jan 2022 20:16:35: 5000000 INFO @ Sat, 15 Jan 2022 20:16:38: 8000000 INFO @ Sat, 15 Jan 2022 20:16:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:16:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:16:39: #1 total tags in treatment: 5198488 INFO @ Sat, 15 Jan 2022 20:16:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:39: #1 tags after filtering in treatment: 4234140 INFO @ Sat, 15 Jan 2022 20:16:39: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 20:16:39: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:39: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:16:39: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:42: 6000000 INFO @ Sat, 15 Jan 2022 20:16:47: 9000000 INFO @ Sat, 15 Jan 2022 20:16:49: 7000000 INFO @ Sat, 15 Jan 2022 20:16:56: 8000000 INFO @ Sat, 15 Jan 2022 20:16:56: 10000000 INFO @ Sat, 15 Jan 2022 20:17:03: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:17:04: 11000000 INFO @ Sat, 15 Jan 2022 20:17:09: 10000000 INFO @ Sat, 15 Jan 2022 20:17:13: 12000000 INFO @ Sat, 15 Jan 2022 20:17:16: 11000000 INFO @ Sat, 15 Jan 2022 20:17:22: 13000000 INFO @ Sat, 15 Jan 2022 20:17:23: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:17:29: 13000000 INFO @ Sat, 15 Jan 2022 20:17:31: 14000000 INFO @ Sat, 15 Jan 2022 20:17:36: 14000000 INFO @ Sat, 15 Jan 2022 20:17:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:36: #1 total tags in treatment: 5198488 INFO @ Sat, 15 Jan 2022 20:17:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:36: #1 tags after filtering in treatment: 4234140 INFO @ Sat, 15 Jan 2022 20:17:36: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 20:17:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:37: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:17:37: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:41: #1 total tags in treatment: 5198488 INFO @ Sat, 15 Jan 2022 20:17:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:41: #1 tags after filtering in treatment: 4234140 INFO @ Sat, 15 Jan 2022 20:17:41: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 20:17:41: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:41: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:17:41: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245417/SRX8245417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling