Job ID = 14520700 SRX = SRX8065705 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39048637 spots for SRR11489728/SRR11489728.sra Written 39048637 spots for SRR11489728/SRR11489728.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:00 39048637 reads; of these: 39048637 (100.00%) were unpaired; of these: 8623161 (22.08%) aligned 0 times 17424122 (44.62%) aligned exactly 1 time 13001354 (33.30%) aligned >1 times 77.92% overall alignment rate Time searching: 00:09:00 Overall time: 00:09:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21503282 / 30425476 = 0.7068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:01:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:01:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:01:32: 1000000 INFO @ Sat, 15 Jan 2022 20:01:43: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:01:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:01:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:01:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:01:53: 3000000 INFO @ Sat, 15 Jan 2022 20:02:02: 1000000 INFO @ Sat, 15 Jan 2022 20:02:04: 4000000 INFO @ Sat, 15 Jan 2022 20:02:12: 2000000 INFO @ Sat, 15 Jan 2022 20:02:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:02:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:02:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:02:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:02:22: 3000000 INFO @ Sat, 15 Jan 2022 20:02:27: 6000000 INFO @ Sat, 15 Jan 2022 20:02:31: 4000000 INFO @ Sat, 15 Jan 2022 20:02:33: 1000000 INFO @ Sat, 15 Jan 2022 20:02:39: 7000000 INFO @ Sat, 15 Jan 2022 20:02:41: 5000000 INFO @ Sat, 15 Jan 2022 20:02:45: 2000000 INFO @ Sat, 15 Jan 2022 20:02:50: 8000000 INFO @ Sat, 15 Jan 2022 20:02:51: 6000000 INFO @ Sat, 15 Jan 2022 20:02:56: 3000000 INFO @ Sat, 15 Jan 2022 20:03:01: 7000000 INFO @ Sat, 15 Jan 2022 20:03:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:03:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:03:01: #1 total tags in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:03:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:03:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:03:01: #1 tags after filtering in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:03:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:03:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:03:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:03:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:03:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:03:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:03:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:03:08: 4000000 INFO @ Sat, 15 Jan 2022 20:03:11: 8000000 INFO @ Sat, 15 Jan 2022 20:03:20: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:03:20: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:03:20: #1 total tags in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:03:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:03:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:03:20: #1 tags after filtering in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:03:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:03:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:03:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:03:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:03:20: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:03:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:03:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:03:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:03:31: 6000000 INFO @ Sat, 15 Jan 2022 20:03:42: 7000000 INFO @ Sat, 15 Jan 2022 20:03:53: 8000000 INFO @ Sat, 15 Jan 2022 20:04:03: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:04:03: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:04:03: #1 total tags in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:04:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:04:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:04:03: #1 tags after filtering in treatment: 8922194 INFO @ Sat, 15 Jan 2022 20:04:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:04:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:04:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:04:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:04:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:04:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:04:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065705/SRX8065705.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling