Job ID = 7108138 SRX = SRX7960935 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 53920901 spots for SRR11359440/SRR11359440.sra Written 53920901 spots for SRR11359440/SRR11359440.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:06 53920901 reads; of these: 53920901 (100.00%) were unpaired; of these: 3675989 (6.82%) aligned 0 times 43140669 (80.01%) aligned exactly 1 time 7104243 (13.18%) aligned >1 times 93.18% overall alignment rate Time searching: 00:20:06 Overall time: 00:20:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 36916418 / 50244912 = 0.7347 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:05:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:05:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:05:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:05:29: 1000000 INFO @ Wed, 22 Jul 2020 14:05:38: 2000000 INFO @ Wed, 22 Jul 2020 14:05:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:05:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:05:50: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:05:50: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:05:57: 4000000 INFO @ Wed, 22 Jul 2020 14:05:59: 1000000 INFO @ Wed, 22 Jul 2020 14:06:07: 5000000 INFO @ Wed, 22 Jul 2020 14:06:07: 2000000 INFO @ Wed, 22 Jul 2020 14:06:16: 3000000 INFO @ Wed, 22 Jul 2020 14:06:17: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:06:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:06:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:06:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:06:24: 4000000 INFO @ Wed, 22 Jul 2020 14:06:27: 7000000 INFO @ Wed, 22 Jul 2020 14:06:29: 1000000 INFO @ Wed, 22 Jul 2020 14:06:33: 5000000 INFO @ Wed, 22 Jul 2020 14:06:36: 8000000 INFO @ Wed, 22 Jul 2020 14:06:38: 2000000 INFO @ Wed, 22 Jul 2020 14:06:42: 6000000 INFO @ Wed, 22 Jul 2020 14:06:46: 9000000 INFO @ Wed, 22 Jul 2020 14:06:46: 3000000 INFO @ Wed, 22 Jul 2020 14:06:50: 7000000 INFO @ Wed, 22 Jul 2020 14:06:55: 4000000 INFO @ Wed, 22 Jul 2020 14:06:56: 10000000 INFO @ Wed, 22 Jul 2020 14:06:59: 8000000 INFO @ Wed, 22 Jul 2020 14:07:04: 5000000 INFO @ Wed, 22 Jul 2020 14:07:06: 11000000 INFO @ Wed, 22 Jul 2020 14:07:08: 9000000 INFO @ Wed, 22 Jul 2020 14:07:12: 6000000 INFO @ Wed, 22 Jul 2020 14:07:16: 12000000 INFO @ Wed, 22 Jul 2020 14:07:17: 10000000 INFO @ Wed, 22 Jul 2020 14:07:21: 7000000 INFO @ Wed, 22 Jul 2020 14:07:25: 11000000 INFO @ Wed, 22 Jul 2020 14:07:25: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:07:29: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 14:07:29: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 14:07:29: #1 total tags in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:07:29: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:07:29: #1 tags after filtering in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:07:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 14:07:29: #1 finished! INFO @ Wed, 22 Jul 2020 14:07:29: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:07:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:07:29: 8000000 INFO @ Wed, 22 Jul 2020 14:07:30: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:07:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:07:33: 12000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:07:38: 9000000 INFO @ Wed, 22 Jul 2020 14:07:42: 13000000 INFO @ Wed, 22 Jul 2020 14:07:44: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 14:07:44: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 14:07:44: #1 total tags in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:07:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:07:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:07:44: #1 tags after filtering in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:07:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 14:07:44: #1 finished! INFO @ Wed, 22 Jul 2020 14:07:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:07:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:07:45: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:07:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:07:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:07:46: 10000000 INFO @ Wed, 22 Jul 2020 14:07:54: 11000000 INFO @ Wed, 22 Jul 2020 14:08:01: 12000000 INFO @ Wed, 22 Jul 2020 14:08:09: 13000000 INFO @ Wed, 22 Jul 2020 14:08:11: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 14:08:11: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 14:08:11: #1 total tags in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:08:11: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:08:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:08:11: #1 tags after filtering in treatment: 13328494 INFO @ Wed, 22 Jul 2020 14:08:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 14:08:11: #1 finished! INFO @ Wed, 22 Jul 2020 14:08:11: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:08:12: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:08:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:08:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960935/SRX7960935.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling