Job ID = 14521376 SRX = SRX7825258 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 10823 READS because READLEN < 1 Read 26026941 spots for SRR11212321/SRR11212321.sra Written 26026941 spots for SRR11212321/SRR11212321.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] 2 unmatched pairs [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5160479 / 25206536 = 0.2047 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:28: 1000000 INFO @ Sat, 15 Jan 2022 21:42:38: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:47: 3000000 INFO @ Sat, 15 Jan 2022 21:42:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:58: 4000000 INFO @ Sat, 15 Jan 2022 21:42:58: 1000000 INFO @ Sat, 15 Jan 2022 21:43:08: 5000000 INFO @ Sat, 15 Jan 2022 21:43:09: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:43:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:43:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:43:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:43:18: 3000000 INFO @ Sat, 15 Jan 2022 21:43:19: 6000000 INFO @ Sat, 15 Jan 2022 21:43:28: 4000000 INFO @ Sat, 15 Jan 2022 21:43:29: 1000000 INFO @ Sat, 15 Jan 2022 21:43:31: 7000000 INFO @ Sat, 15 Jan 2022 21:43:39: 5000000 INFO @ Sat, 15 Jan 2022 21:43:39: 2000000 INFO @ Sat, 15 Jan 2022 21:43:42: 8000000 INFO @ Sat, 15 Jan 2022 21:43:49: 3000000 INFO @ Sat, 15 Jan 2022 21:43:49: 6000000 INFO @ Sat, 15 Jan 2022 21:43:53: 9000000 INFO @ Sat, 15 Jan 2022 21:43:59: 4000000 INFO @ Sat, 15 Jan 2022 21:43:59: 7000000 INFO @ Sat, 15 Jan 2022 21:44:04: 10000000 INFO @ Sat, 15 Jan 2022 21:44:09: 5000000 INFO @ Sat, 15 Jan 2022 21:44:09: 8000000 INFO @ Sat, 15 Jan 2022 21:44:15: 11000000 INFO @ Sat, 15 Jan 2022 21:44:19: 6000000 INFO @ Sat, 15 Jan 2022 21:44:19: 9000000 INFO @ Sat, 15 Jan 2022 21:44:26: 12000000 INFO @ Sat, 15 Jan 2022 21:44:29: 7000000 INFO @ Sat, 15 Jan 2022 21:44:29: 10000000 INFO @ Sat, 15 Jan 2022 21:44:37: 13000000 INFO @ Sat, 15 Jan 2022 21:44:39: 8000000 INFO @ Sat, 15 Jan 2022 21:44:39: 11000000 INFO @ Sat, 15 Jan 2022 21:44:48: 14000000 INFO @ Sat, 15 Jan 2022 21:44:49: 9000000 INFO @ Sat, 15 Jan 2022 21:44:49: 12000000 INFO @ Sat, 15 Jan 2022 21:44:58: 10000000 INFO @ Sat, 15 Jan 2022 21:44:59: 15000000 INFO @ Sat, 15 Jan 2022 21:44:59: 13000000 INFO @ Sat, 15 Jan 2022 21:45:08: 11000000 INFO @ Sat, 15 Jan 2022 21:45:09: 14000000 INFO @ Sat, 15 Jan 2022 21:45:09: 16000000 INFO @ Sat, 15 Jan 2022 21:45:18: 12000000 INFO @ Sat, 15 Jan 2022 21:45:18: 15000000 INFO @ Sat, 15 Jan 2022 21:45:20: 17000000 INFO @ Sat, 15 Jan 2022 21:45:28: 13000000 INFO @ Sat, 15 Jan 2022 21:45:28: 16000000 INFO @ Sat, 15 Jan 2022 21:45:31: 18000000 INFO @ Sat, 15 Jan 2022 21:45:38: 14000000 INFO @ Sat, 15 Jan 2022 21:45:38: 17000000 INFO @ Sat, 15 Jan 2022 21:45:42: 19000000 INFO @ Sat, 15 Jan 2022 21:45:48: 15000000 INFO @ Sat, 15 Jan 2022 21:45:48: 18000000 INFO @ Sat, 15 Jan 2022 21:45:53: 20000000 INFO @ Sat, 15 Jan 2022 21:45:58: 16000000 INFO @ Sat, 15 Jan 2022 21:45:58: 19000000 INFO @ Sat, 15 Jan 2022 21:46:03: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:46:08: 17000000 INFO @ Sat, 15 Jan 2022 21:46:08: 20000000 INFO @ Sat, 15 Jan 2022 21:46:14: 22000000 INFO @ Sat, 15 Jan 2022 21:46:18: 18000000 INFO @ Sat, 15 Jan 2022 21:46:18: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:46:24: 23000000 INFO @ Sat, 15 Jan 2022 21:46:28: 19000000 INFO @ Sat, 15 Jan 2022 21:46:28: 22000000 INFO @ Sat, 15 Jan 2022 21:46:35: 24000000 INFO @ Sat, 15 Jan 2022 21:46:38: 20000000 INFO @ Sat, 15 Jan 2022 21:46:38: 23000000 INFO @ Sat, 15 Jan 2022 21:46:46: 25000000 INFO @ Sat, 15 Jan 2022 21:46:48: 21000000 INFO @ Sat, 15 Jan 2022 21:46:48: 24000000 INFO @ Sat, 15 Jan 2022 21:46:56: 26000000 INFO @ Sat, 15 Jan 2022 21:46:58: 22000000 INFO @ Sat, 15 Jan 2022 21:46:58: 25000000 INFO @ Sat, 15 Jan 2022 21:47:07: 27000000 INFO @ Sat, 15 Jan 2022 21:47:07: 23000000 INFO @ Sat, 15 Jan 2022 21:47:07: 26000000 INFO @ Sat, 15 Jan 2022 21:47:17: 24000000 INFO @ Sat, 15 Jan 2022 21:47:17: 27000000 INFO @ Sat, 15 Jan 2022 21:47:17: 28000000 INFO @ Sat, 15 Jan 2022 21:47:27: 25000000 INFO @ Sat, 15 Jan 2022 21:47:27: 28000000 INFO @ Sat, 15 Jan 2022 21:47:28: 29000000 INFO @ Sat, 15 Jan 2022 21:47:37: 26000000 INFO @ Sat, 15 Jan 2022 21:47:37: 29000000 INFO @ Sat, 15 Jan 2022 21:47:39: 30000000 INFO @ Sat, 15 Jan 2022 21:47:47: 27000000 INFO @ Sat, 15 Jan 2022 21:47:47: 30000000 INFO @ Sat, 15 Jan 2022 21:47:50: 31000000 INFO @ Sat, 15 Jan 2022 21:47:56: 28000000 INFO @ Sat, 15 Jan 2022 21:47:56: 31000000 INFO @ Sat, 15 Jan 2022 21:48:01: 32000000 INFO @ Sat, 15 Jan 2022 21:48:06: 29000000 INFO @ Sat, 15 Jan 2022 21:48:06: 32000000 INFO @ Sat, 15 Jan 2022 21:48:12: 33000000 INFO @ Sat, 15 Jan 2022 21:48:16: 33000000 INFO @ Sat, 15 Jan 2022 21:48:16: 30000000 INFO @ Sat, 15 Jan 2022 21:48:23: 34000000 INFO @ Sat, 15 Jan 2022 21:48:26: 31000000 INFO @ Sat, 15 Jan 2022 21:48:26: 34000000 INFO @ Sat, 15 Jan 2022 21:48:34: 35000000 INFO @ Sat, 15 Jan 2022 21:48:35: 32000000 INFO @ Sat, 15 Jan 2022 21:48:36: 35000000 INFO @ Sat, 15 Jan 2022 21:48:44: 36000000 INFO @ Sat, 15 Jan 2022 21:48:45: 33000000 INFO @ Sat, 15 Jan 2022 21:48:46: 36000000 INFO @ Sat, 15 Jan 2022 21:48:55: 37000000 INFO @ Sat, 15 Jan 2022 21:48:55: 34000000 INFO @ Sat, 15 Jan 2022 21:48:56: 37000000 INFO @ Sat, 15 Jan 2022 21:49:05: 35000000 INFO @ Sat, 15 Jan 2022 21:49:05: 38000000 INFO @ Sat, 15 Jan 2022 21:49:06: 38000000 INFO @ Sat, 15 Jan 2022 21:49:14: 36000000 INFO @ Sat, 15 Jan 2022 21:49:16: 39000000 INFO @ Sat, 15 Jan 2022 21:49:17: 39000000 INFO @ Sat, 15 Jan 2022 21:49:23: 37000000 INFO @ Sat, 15 Jan 2022 21:49:27: 40000000 INFO @ Sat, 15 Jan 2022 21:49:28: 40000000 INFO @ Sat, 15 Jan 2022 21:49:33: 38000000 INFO @ Sat, 15 Jan 2022 21:49:34: #1 tag size is determined as 140 bps INFO @ Sat, 15 Jan 2022 21:49:34: #1 tag size = 140 INFO @ Sat, 15 Jan 2022 21:49:34: #1 total tags in treatment: 19908687 INFO @ Sat, 15 Jan 2022 21:49:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:34: #1 tags after filtering in treatment: 12607208 INFO @ Sat, 15 Jan 2022 21:49:34: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:49:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:35: #1 tag size is determined as 140 bps INFO @ Sat, 15 Jan 2022 21:49:35: #1 tag size = 140 INFO @ Sat, 15 Jan 2022 21:49:35: #1 total tags in treatment: 19908687 INFO @ Sat, 15 Jan 2022 21:49:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:49:35: #1 tags after filtering in treatment: 12607208 INFO @ Sat, 15 Jan 2022 21:49:35: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:49:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:49:42: 39000000 INFO @ Sat, 15 Jan 2022 21:49:50: 40000000 INFO @ Sat, 15 Jan 2022 21:49:55: #1 tag size is determined as 140 bps INFO @ Sat, 15 Jan 2022 21:49:55: #1 tag size = 140 INFO @ Sat, 15 Jan 2022 21:49:55: #1 total tags in treatment: 19908687 INFO @ Sat, 15 Jan 2022 21:49:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:55: #1 tags after filtering in treatment: 12607208 INFO @ Sat, 15 Jan 2022 21:49:55: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:49:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825258/SRX7825258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling