Job ID = 14521968 SRX = SRX7756557 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13065126 spots for SRR11119563/SRR11119563.sra Written 13065126 spots for SRR11119563/SRR11119563.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:30 13065126 reads; of these: 13065126 (100.00%) were paired; of these: 2698968 (20.66%) aligned concordantly 0 times 9663057 (73.96%) aligned concordantly exactly 1 time 703101 (5.38%) aligned concordantly >1 times ---- 2698968 pairs aligned concordantly 0 times; of these: 1113705 (41.26%) aligned discordantly 1 time ---- 1585263 pairs aligned 0 times concordantly or discordantly; of these: 3170526 mates make up the pairs; of these: 2544725 (80.26%) aligned 0 times 406042 (12.81%) aligned exactly 1 time 219759 (6.93%) aligned >1 times 90.26% overall alignment rate Time searching: 00:13:30 Overall time: 00:13:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1643772 / 11464739 = 0.1434 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:27: 1000000 INFO @ Sat, 15 Jan 2022 22:22:35: 2000000 INFO @ Sat, 15 Jan 2022 22:22:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:50: 4000000 INFO @ Sat, 15 Jan 2022 22:23:00: 5000000 INFO @ Sat, 15 Jan 2022 22:23:01: 1000000 INFO @ Sat, 15 Jan 2022 22:23:10: 6000000 INFO @ Sat, 15 Jan 2022 22:23:12: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:23:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:23:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:23:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:23:21: 7000000 INFO @ Sat, 15 Jan 2022 22:23:23: 3000000 INFO @ Sat, 15 Jan 2022 22:23:31: 8000000 INFO @ Sat, 15 Jan 2022 22:23:32: 1000000 INFO @ Sat, 15 Jan 2022 22:23:35: 4000000 INFO @ Sat, 15 Jan 2022 22:23:41: 9000000 INFO @ Sat, 15 Jan 2022 22:23:43: 2000000 INFO @ Sat, 15 Jan 2022 22:23:48: 5000000 INFO @ Sat, 15 Jan 2022 22:23:52: 10000000 INFO @ Sat, 15 Jan 2022 22:23:55: 3000000 INFO @ Sat, 15 Jan 2022 22:24:00: 6000000 INFO @ Sat, 15 Jan 2022 22:24:02: 11000000 INFO @ Sat, 15 Jan 2022 22:24:06: 4000000 INFO @ Sat, 15 Jan 2022 22:24:12: 7000000 INFO @ Sat, 15 Jan 2022 22:24:13: 12000000 INFO @ Sat, 15 Jan 2022 22:24:18: 5000000 INFO @ Sat, 15 Jan 2022 22:24:24: 13000000 INFO @ Sat, 15 Jan 2022 22:24:25: 8000000 INFO @ Sat, 15 Jan 2022 22:24:29: 6000000 INFO @ Sat, 15 Jan 2022 22:24:34: 14000000 INFO @ Sat, 15 Jan 2022 22:24:37: 9000000 INFO @ Sat, 15 Jan 2022 22:24:40: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:24:45: 15000000 INFO @ Sat, 15 Jan 2022 22:24:49: 10000000 INFO @ Sat, 15 Jan 2022 22:24:52: 8000000 INFO @ Sat, 15 Jan 2022 22:24:55: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:25:01: 11000000 INFO @ Sat, 15 Jan 2022 22:25:03: 9000000 INFO @ Sat, 15 Jan 2022 22:25:06: 17000000 INFO @ Sat, 15 Jan 2022 22:25:13: 12000000 INFO @ Sat, 15 Jan 2022 22:25:15: 10000000 INFO @ Sat, 15 Jan 2022 22:25:16: 18000000 INFO @ Sat, 15 Jan 2022 22:25:24: 13000000 INFO @ Sat, 15 Jan 2022 22:25:27: 19000000 INFO @ Sat, 15 Jan 2022 22:25:27: 11000000 INFO @ Sat, 15 Jan 2022 22:25:36: 14000000 INFO @ Sat, 15 Jan 2022 22:25:37: 20000000 INFO @ Sat, 15 Jan 2022 22:25:39: 12000000 INFO @ Sat, 15 Jan 2022 22:25:40: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:25:40: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:25:40: #1 total tags in treatment: 8852916 INFO @ Sat, 15 Jan 2022 22:25:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:25:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:25:40: #1 tags after filtering in treatment: 6521477 INFO @ Sat, 15 Jan 2022 22:25:40: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:25:40: #1 finished! INFO @ Sat, 15 Jan 2022 22:25:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:25:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:25:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:25:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:25:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:25:48: 15000000 INFO @ Sat, 15 Jan 2022 22:25:50: 13000000 INFO @ Sat, 15 Jan 2022 22:26:01: 16000000 INFO @ Sat, 15 Jan 2022 22:26:02: 14000000 INFO @ Sat, 15 Jan 2022 22:26:13: 17000000 INFO @ Sat, 15 Jan 2022 22:26:13: 15000000 INFO @ Sat, 15 Jan 2022 22:26:25: 18000000 INFO @ Sat, 15 Jan 2022 22:26:25: 16000000 INFO @ Sat, 15 Jan 2022 22:26:37: 19000000 INFO @ Sat, 15 Jan 2022 22:26:38: 17000000 INFO @ Sat, 15 Jan 2022 22:26:49: 20000000 INFO @ Sat, 15 Jan 2022 22:26:50: 18000000 INFO @ Sat, 15 Jan 2022 22:26:52: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:26:52: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:26:52: #1 total tags in treatment: 8852916 INFO @ Sat, 15 Jan 2022 22:26:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:26:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:26:52: #1 tags after filtering in treatment: 6521477 INFO @ Sat, 15 Jan 2022 22:26:52: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:26:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:26:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:26:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:26:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:26:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:26:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:27:00: 19000000 INFO @ Sat, 15 Jan 2022 22:27:10: 20000000 INFO @ Sat, 15 Jan 2022 22:27:13: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:27:13: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:27:13: #1 total tags in treatment: 8852916 INFO @ Sat, 15 Jan 2022 22:27:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:27:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:27:13: #1 tags after filtering in treatment: 6521477 INFO @ Sat, 15 Jan 2022 22:27:13: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:27:13: #1 finished! INFO @ Sat, 15 Jan 2022 22:27:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:27:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:27:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:27:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:27:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756557/SRX7756557.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling