Job ID = 5791710 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,350,277 reads read : 5,350,277 reads written : 5,350,277 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:36 5350277 reads; of these: 5350277 (100.00%) were unpaired; of these: 1582565 (29.58%) aligned 0 times 1461539 (27.32%) aligned exactly 1 time 2306173 (43.10%) aligned >1 times 70.42% overall alignment rate Time searching: 00:00:36 Overall time: 00:00:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2225743 / 3767712 = 0.5907 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:36:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:35: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:35: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:36:41: 1000000 INFO @ Wed, 22 Apr 2020 10:36:44: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:36:44: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:36:44: #1 total tags in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:36:44: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:36:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:36:44: #1 tags after filtering in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:36:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:36:44: #1 finished! INFO @ Wed, 22 Apr 2020 10:36:44: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:36:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:36:44: #2 number of paired peaks: 64 WARNING @ Wed, 22 Apr 2020 10:36:44: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:36:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:37:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:37:05: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:37:05: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:37:11: 1000000 INFO @ Wed, 22 Apr 2020 10:37:14: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:37:14: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:37:14: #1 total tags in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:37:14: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:37:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:37:14: #1 tags after filtering in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:37:14: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:37:14: #1 finished! INFO @ Wed, 22 Apr 2020 10:37:14: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:37:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:37:14: #2 number of paired peaks: 64 WARNING @ Wed, 22 Apr 2020 10:37:14: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:37:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:37:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:37:35: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:37:35: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:37:41: 1000000 INFO @ Wed, 22 Apr 2020 10:37:44: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:37:44: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:37:44: #1 total tags in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:37:44: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:37:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:37:44: #1 tags after filtering in treatment: 1541969 INFO @ Wed, 22 Apr 2020 10:37:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:37:44: #1 finished! INFO @ Wed, 22 Apr 2020 10:37:44: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:37:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:37:44: #2 number of paired peaks: 64 WARNING @ Wed, 22 Apr 2020 10:37:44: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:37:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671081/SRX7671081.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。