Job ID = 10223943 SRX = SRX7496392 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4930148 spots for SRR10823003/SRR10823003.sra Written 4930148 spots for SRR10823003/SRR10823003.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:46 4930148 reads; of these: 4930148 (100.00%) were paired; of these: 2107901 (42.76%) aligned concordantly 0 times 2697054 (54.71%) aligned concordantly exactly 1 time 125193 (2.54%) aligned concordantly >1 times ---- 2107901 pairs aligned concordantly 0 times; of these: 59259 (2.81%) aligned discordantly 1 time ---- 2048642 pairs aligned 0 times concordantly or discordantly; of these: 4097284 mates make up the pairs; of these: 2213911 (54.03%) aligned 0 times 1782082 (43.49%) aligned exactly 1 time 101291 (2.47%) aligned >1 times 77.55% overall alignment rate Time searching: 00:01:46 Overall time: 00:01:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 74480 / 2879083 = 0.0259 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:57:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:57:34: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:57:34: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:57:39: 1000000 INFO @ Fri, 16 Oct 2020 08:57:44: 2000000 INFO @ Fri, 16 Oct 2020 08:57:49: 3000000 INFO @ Fri, 16 Oct 2020 08:57:54: 4000000 INFO @ Fri, 16 Oct 2020 08:57:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:58:03: 6000000 INFO @ Fri, 16 Oct 2020 08:58:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:58:04: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:58:04: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:58:09: 7000000 INFO @ Fri, 16 Oct 2020 08:58:11: 1000000 INFO @ Fri, 16 Oct 2020 08:58:11: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:58:11: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:58:11: #1 total tags in treatment: 2748163 INFO @ Fri, 16 Oct 2020 08:58:11: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:58:11: #1 tags after filtering in treatment: 1918460 INFO @ Fri, 16 Oct 2020 08:58:11: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 16 Oct 2020 08:58:11: #1 finished! INFO @ Fri, 16 Oct 2020 08:58:11: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:58:12: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:58:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:58:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:58:16: 2000000 INFO @ Fri, 16 Oct 2020 08:58:21: 3000000 INFO @ Fri, 16 Oct 2020 08:58:26: 4000000 INFO @ Fri, 16 Oct 2020 08:58:31: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:58:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:58:34: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:58:34: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:58:36: 6000000 INFO @ Fri, 16 Oct 2020 08:58:40: 1000000 INFO @ Fri, 16 Oct 2020 08:58:41: 7000000 INFO @ Fri, 16 Oct 2020 08:58:44: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:58:44: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:58:44: #1 total tags in treatment: 2748163 INFO @ Fri, 16 Oct 2020 08:58:44: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:58:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:58:44: #1 tags after filtering in treatment: 1918460 INFO @ Fri, 16 Oct 2020 08:58:44: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 16 Oct 2020 08:58:44: #1 finished! INFO @ Fri, 16 Oct 2020 08:58:44: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:58:44: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:58:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:58:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:58:46: 2000000 INFO @ Fri, 16 Oct 2020 08:58:51: 3000000 INFO @ Fri, 16 Oct 2020 08:58:55: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:59:00: 5000000 INFO @ Fri, 16 Oct 2020 08:59:05: 6000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:59:10: 7000000 INFO @ Fri, 16 Oct 2020 08:59:13: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:59:13: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:59:13: #1 total tags in treatment: 2748163 INFO @ Fri, 16 Oct 2020 08:59:13: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:59:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:59:13: #1 tags after filtering in treatment: 1918460 INFO @ Fri, 16 Oct 2020 08:59:13: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 16 Oct 2020 08:59:13: #1 finished! INFO @ Fri, 16 Oct 2020 08:59:13: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:59:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:59:13: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:59:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:59:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496392/SRX7496392.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling