Job ID = 7107758 SRX = SRX7217635 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19608621 spots for SRR10533712/SRR10533712.sra Written 19608621 spots for SRR10533712/SRR10533712.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:04 19608621 reads; of these: 19608621 (100.00%) were paired; of these: 4047374 (20.64%) aligned concordantly 0 times 13166595 (67.15%) aligned concordantly exactly 1 time 2394652 (12.21%) aligned concordantly >1 times ---- 4047374 pairs aligned concordantly 0 times; of these: 2219238 (54.83%) aligned discordantly 1 time ---- 1828136 pairs aligned 0 times concordantly or discordantly; of these: 3656272 mates make up the pairs; of these: 2216384 (60.62%) aligned 0 times 462542 (12.65%) aligned exactly 1 time 977346 (26.73%) aligned >1 times 94.35% overall alignment rate Time searching: 00:27:04 Overall time: 00:27:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3158381 / 17392068 = 0.1816 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:52:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:52:33: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:52:33: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:52:39: 1000000 INFO @ Wed, 22 Jul 2020 13:52:45: 2000000 INFO @ Wed, 22 Jul 2020 13:52:51: 3000000 INFO @ Wed, 22 Jul 2020 13:52:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:53:03: 5000000 INFO @ Wed, 22 Jul 2020 13:53:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:53:03: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:53:03: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:53:10: 6000000 INFO @ Wed, 22 Jul 2020 13:53:10: 1000000 INFO @ Wed, 22 Jul 2020 13:53:16: 7000000 INFO @ Wed, 22 Jul 2020 13:53:17: 2000000 INFO @ Wed, 22 Jul 2020 13:53:22: 8000000 INFO @ Wed, 22 Jul 2020 13:53:24: 3000000 INFO @ Wed, 22 Jul 2020 13:53:28: 9000000 BedGraph に変換中... INFO @ Wed, 22 Jul 2020 13:53:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:53:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:53:33: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:53:33: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:53:34: 10000000 INFO @ Wed, 22 Jul 2020 13:53:39: 5000000 INFO @ Wed, 22 Jul 2020 13:53:40: 1000000 INFO @ Wed, 22 Jul 2020 13:53:41: 11000000 INFO @ Wed, 22 Jul 2020 13:53:45: 6000000 INFO @ Wed, 22 Jul 2020 13:53:47: 12000000 INFO @ Wed, 22 Jul 2020 13:53:48: 2000000 INFO @ Wed, 22 Jul 2020 13:53:52: 7000000 INFO @ Wed, 22 Jul 2020 13:53:53: 13000000 INFO @ Wed, 22 Jul 2020 13:53:55: 3000000 INFO @ Wed, 22 Jul 2020 13:53:59: 14000000 INFO @ Wed, 22 Jul 2020 13:54:00: 8000000 INFO @ Wed, 22 Jul 2020 13:54:02: 4000000 INFO @ Wed, 22 Jul 2020 13:54:05: 15000000 INFO @ Wed, 22 Jul 2020 13:54:07: 9000000 INFO @ Wed, 22 Jul 2020 13:54:09: 5000000 INFO @ Wed, 22 Jul 2020 13:54:11: 16000000 INFO @ Wed, 22 Jul 2020 13:54:14: 10000000 INFO @ Wed, 22 Jul 2020 13:54:16: 6000000 INFO @ Wed, 22 Jul 2020 13:54:18: 17000000 INFO @ Wed, 22 Jul 2020 13:54:21: 11000000 INFO @ Wed, 22 Jul 2020 13:54:23: 7000000 INFO @ Wed, 22 Jul 2020 13:54:24: 18000000 INFO @ Wed, 22 Jul 2020 13:54:28: 12000000 INFO @ Wed, 22 Jul 2020 13:54:30: 19000000 INFO @ Wed, 22 Jul 2020 13:54:30: 8000000 INFO @ Wed, 22 Jul 2020 13:54:35: 13000000 INFO @ Wed, 22 Jul 2020 13:54:36: 20000000 INFO @ Wed, 22 Jul 2020 13:54:37: 9000000 INFO @ Wed, 22 Jul 2020 13:54:42: 21000000 INFO @ Wed, 22 Jul 2020 13:54:42: 14000000 INFO @ Wed, 22 Jul 2020 13:54:45: 10000000 INFO @ Wed, 22 Jul 2020 13:54:48: 22000000 INFO @ Wed, 22 Jul 2020 13:54:49: 15000000 INFO @ Wed, 22 Jul 2020 13:54:52: 11000000 INFO @ Wed, 22 Jul 2020 13:54:54: 23000000 INFO @ Wed, 22 Jul 2020 13:54:56: 16000000 INFO @ Wed, 22 Jul 2020 13:54:59: 12000000 INFO @ Wed, 22 Jul 2020 13:55:00: 24000000 INFO @ Wed, 22 Jul 2020 13:55:04: 17000000 INFO @ Wed, 22 Jul 2020 13:55:06: 13000000 INFO @ Wed, 22 Jul 2020 13:55:06: 25000000 INFO @ Wed, 22 Jul 2020 13:55:11: 18000000 INFO @ Wed, 22 Jul 2020 13:55:12: 26000000 INFO @ Wed, 22 Jul 2020 13:55:13: 14000000 INFO @ Wed, 22 Jul 2020 13:55:18: 19000000 INFO @ Wed, 22 Jul 2020 13:55:18: 27000000 INFO @ Wed, 22 Jul 2020 13:55:20: 15000000 INFO @ Wed, 22 Jul 2020 13:55:24: 28000000 INFO @ Wed, 22 Jul 2020 13:55:25: 20000000 INFO @ Wed, 22 Jul 2020 13:55:28: 16000000 INFO @ Wed, 22 Jul 2020 13:55:30: 29000000 INFO @ Wed, 22 Jul 2020 13:55:32: 21000000 INFO @ Wed, 22 Jul 2020 13:55:35: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:55:36: 30000000 INFO @ Wed, 22 Jul 2020 13:55:39: 22000000 INFO @ Wed, 22 Jul 2020 13:55:41: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:55:41: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:55:41: #1 total tags in treatment: 12725146 INFO @ Wed, 22 Jul 2020 13:55:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:55:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:55:41: #1 tags after filtering in treatment: 8782579 INFO @ Wed, 22 Jul 2020 13:55:41: #1 Redundant rate of treatment: 0.31 INFO @ Wed, 22 Jul 2020 13:55:41: #1 finished! INFO @ Wed, 22 Jul 2020 13:55:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:55:41: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:55:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:55:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:55:42: 18000000 INFO @ Wed, 22 Jul 2020 13:55:46: 23000000 INFO @ Wed, 22 Jul 2020 13:55:49: 19000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:55:53: 24000000 INFO @ Wed, 22 Jul 2020 13:55:56: 20000000 INFO @ Wed, 22 Jul 2020 13:56:00: 25000000 INFO @ Wed, 22 Jul 2020 13:56:03: 21000000 INFO @ Wed, 22 Jul 2020 13:56:07: 26000000 INFO @ Wed, 22 Jul 2020 13:56:10: 22000000 INFO @ Wed, 22 Jul 2020 13:56:14: 27000000 INFO @ Wed, 22 Jul 2020 13:56:17: 23000000 INFO @ Wed, 22 Jul 2020 13:56:21: 28000000 INFO @ Wed, 22 Jul 2020 13:56:24: 24000000 INFO @ Wed, 22 Jul 2020 13:56:27: 29000000 INFO @ Wed, 22 Jul 2020 13:56:31: 25000000 INFO @ Wed, 22 Jul 2020 13:56:34: 30000000 INFO @ Wed, 22 Jul 2020 13:56:38: 26000000 INFO @ Wed, 22 Jul 2020 13:56:39: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:56:39: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:56:39: #1 total tags in treatment: 12725146 INFO @ Wed, 22 Jul 2020 13:56:39: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:56:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:56:39: #1 tags after filtering in treatment: 8782579 INFO @ Wed, 22 Jul 2020 13:56:39: #1 Redundant rate of treatment: 0.31 INFO @ Wed, 22 Jul 2020 13:56:39: #1 finished! INFO @ Wed, 22 Jul 2020 13:56:39: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:56:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:56:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:56:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:56:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:56:45: 27000000 INFO @ Wed, 22 Jul 2020 13:56:52: 28000000 INFO @ Wed, 22 Jul 2020 13:56:59: 29000000 INFO @ Wed, 22 Jul 2020 13:57:06: 30000000 INFO @ Wed, 22 Jul 2020 13:57:11: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:57:11: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:57:11: #1 total tags in treatment: 12725146 INFO @ Wed, 22 Jul 2020 13:57:11: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:57:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:57:11: #1 tags after filtering in treatment: 8782579 INFO @ Wed, 22 Jul 2020 13:57:11: #1 Redundant rate of treatment: 0.31 INFO @ Wed, 22 Jul 2020 13:57:11: #1 finished! INFO @ Wed, 22 Jul 2020 13:57:11: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:57:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:57:12: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:57:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:57:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217635/SRX7217635.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling