Job ID = 14520727 SRX = SRX7119867 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22398437 spots for SRR10423774/SRR10423774.sra Written 22398437 spots for SRR10423774/SRR10423774.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 22398437 reads; of these: 22398437 (100.00%) were unpaired; of these: 1621465 (7.24%) aligned 0 times 19340764 (86.35%) aligned exactly 1 time 1436208 (6.41%) aligned >1 times 92.76% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14143625 / 20776972 = 0.6807 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:13: 1000000 INFO @ Sat, 15 Jan 2022 19:49:18: 2000000 INFO @ Sat, 15 Jan 2022 19:49:23: 3000000 INFO @ Sat, 15 Jan 2022 19:49:29: 4000000 INFO @ Sat, 15 Jan 2022 19:49:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:40: 6000000 INFO @ Sat, 15 Jan 2022 19:49:43: 1000000 INFO @ Sat, 15 Jan 2022 19:49:43: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:49:43: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:49:43: #1 total tags in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:49:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:44: #1 tags after filtering in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:49:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:44: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:49: 2000000 INFO @ Sat, 15 Jan 2022 19:49:54: 3000000 INFO @ Sat, 15 Jan 2022 19:50:00: 4000000 INFO @ Sat, 15 Jan 2022 19:50:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:11: 6000000 INFO @ Sat, 15 Jan 2022 19:50:13: 1000000 INFO @ Sat, 15 Jan 2022 19:50:14: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:14: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:14: #1 total tags in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:50:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:14: #1 tags after filtering in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:50:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:14: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:19: 2000000 INFO @ Sat, 15 Jan 2022 19:50:24: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:50:29: 4000000 INFO @ Sat, 15 Jan 2022 19:50:34: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:50:39: 6000000 INFO @ Sat, 15 Jan 2022 19:50:42: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:42: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:42: #1 total tags in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:50:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:42: #1 tags after filtering in treatment: 6633347 INFO @ Sat, 15 Jan 2022 19:50:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119867/SRX7119867.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling