Job ID = 4289598 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,329,955 reads read : 50,659,910 reads written : 25,329,955 reads 0-length : 25,329,955 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 25329955 reads; of these: 25329955 (100.00%) were unpaired; of these: 6438926 (25.42%) aligned 0 times 10774025 (42.53%) aligned exactly 1 time 8117004 (32.05%) aligned >1 times 74.58% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 13133893 / 18891029 = 0.6952 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 15:10:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:10:26: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:10:26: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:10:36: 1000000 INFO @ Tue, 10 Dec 2019 15:10:46: 2000000 INFO @ Tue, 10 Dec 2019 15:10:55: 3000000 INFO @ Tue, 10 Dec 2019 15:10:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:10:56: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:10:56: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:11:05: 4000000 INFO @ Tue, 10 Dec 2019 15:11:05: 1000000 INFO @ Tue, 10 Dec 2019 15:11:14: 2000000 INFO @ Tue, 10 Dec 2019 15:11:15: 5000000 INFO @ Tue, 10 Dec 2019 15:11:23: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:11:23: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:11:23: #1 total tags in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:11:23: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:11:23: #1 tags after filtering in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:11:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:11:23: #1 finished! INFO @ Tue, 10 Dec 2019 15:11:23: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:11:23: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... INFO @ Tue, 10 Dec 2019 15:11:23: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:11:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:11:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:11:24: 3000000 INFO @ Tue, 10 Dec 2019 15:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:11:26: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:11:26: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:11:32: 4000000 INFO @ Tue, 10 Dec 2019 15:11:37: 1000000 INFO @ Tue, 10 Dec 2019 15:11:41: 5000000 INFO @ Tue, 10 Dec 2019 15:11:45: 2000000 INFO @ Tue, 10 Dec 2019 15:11:47: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:11:47: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:11:47: #1 total tags in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:11:47: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:11:47: #1 tags after filtering in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:11:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:11:47: #1 finished! INFO @ Tue, 10 Dec 2019 15:11:47: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:11:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:11:48: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:11:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:11:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:11:54: 3000000 INFO @ Tue, 10 Dec 2019 15:12:03: 4000000 INFO @ Tue, 10 Dec 2019 15:12:12: 5000000 INFO @ Tue, 10 Dec 2019 15:12:18: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:12:18: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:12:18: #1 total tags in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:12:18: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:12:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:12:18: #1 tags after filtering in treatment: 5757136 INFO @ Tue, 10 Dec 2019 15:12:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:12:18: #1 finished! INFO @ Tue, 10 Dec 2019 15:12:18: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:12:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:12:19: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:12:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:12:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106970/SRX7106970.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。