Job ID = 7102968 SRX = SRX6911522 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11059584 spots for SRR10191165/SRR10191165.sra Written 11059584 spots for SRR10191165/SRR10191165.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:12 11059584 reads; of these: 11059584 (100.00%) were paired; of these: 2398740 (21.69%) aligned concordantly 0 times 7291405 (65.93%) aligned concordantly exactly 1 time 1369439 (12.38%) aligned concordantly >1 times ---- 2398740 pairs aligned concordantly 0 times; of these: 804527 (33.54%) aligned discordantly 1 time ---- 1594213 pairs aligned 0 times concordantly or discordantly; of these: 3188426 mates make up the pairs; of these: 2468333 (77.42%) aligned 0 times 387655 (12.16%) aligned exactly 1 time 332438 (10.43%) aligned >1 times 88.84% overall alignment rate Time searching: 00:11:12 Overall time: 00:11:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 847086 / 9457022 = 0.0896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:40:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:40:10: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:40:10: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:40:19: 1000000 INFO @ Wed, 22 Jul 2020 12:40:27: 2000000 INFO @ Wed, 22 Jul 2020 12:40:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:40:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:40:40: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:40:40: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:40:44: 4000000 INFO @ Wed, 22 Jul 2020 12:40:49: 1000000 INFO @ Wed, 22 Jul 2020 12:40:53: 5000000 INFO @ Wed, 22 Jul 2020 12:40:58: 2000000 INFO @ Wed, 22 Jul 2020 12:41:01: 6000000 INFO @ Wed, 22 Jul 2020 12:41:06: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:41:10: 7000000 INFO @ Wed, 22 Jul 2020 12:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:41:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:41:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:41:16: 4000000 INFO @ Wed, 22 Jul 2020 12:41:20: 8000000 INFO @ Wed, 22 Jul 2020 12:41:20: 1000000 INFO @ Wed, 22 Jul 2020 12:41:26: 5000000 INFO @ Wed, 22 Jul 2020 12:41:29: 9000000 INFO @ Wed, 22 Jul 2020 12:41:30: 2000000 INFO @ Wed, 22 Jul 2020 12:41:36: 6000000 INFO @ Wed, 22 Jul 2020 12:41:39: 10000000 INFO @ Wed, 22 Jul 2020 12:41:39: 3000000 INFO @ Wed, 22 Jul 2020 12:41:45: 7000000 INFO @ Wed, 22 Jul 2020 12:41:48: 11000000 INFO @ Wed, 22 Jul 2020 12:41:49: 4000000 INFO @ Wed, 22 Jul 2020 12:41:55: 8000000 INFO @ Wed, 22 Jul 2020 12:41:58: 12000000 INFO @ Wed, 22 Jul 2020 12:41:58: 5000000 INFO @ Wed, 22 Jul 2020 12:42:05: 9000000 INFO @ Wed, 22 Jul 2020 12:42:07: 13000000 INFO @ Wed, 22 Jul 2020 12:42:07: 6000000 INFO @ Wed, 22 Jul 2020 12:42:15: 10000000 INFO @ Wed, 22 Jul 2020 12:42:16: 14000000 INFO @ Wed, 22 Jul 2020 12:42:17: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:42:25: 11000000 INFO @ Wed, 22 Jul 2020 12:42:26: 15000000 INFO @ Wed, 22 Jul 2020 12:42:26: 8000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:42:35: 12000000 INFO @ Wed, 22 Jul 2020 12:42:35: 16000000 INFO @ Wed, 22 Jul 2020 12:42:36: 9000000 INFO @ Wed, 22 Jul 2020 12:42:45: 13000000 INFO @ Wed, 22 Jul 2020 12:42:45: 17000000 INFO @ Wed, 22 Jul 2020 12:42:45: 10000000 INFO @ Wed, 22 Jul 2020 12:42:54: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:42:54: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:42:54: #1 total tags in treatment: 7870228 INFO @ Wed, 22 Jul 2020 12:42:54: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:42:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:42:54: #1 tags after filtering in treatment: 5192828 INFO @ Wed, 22 Jul 2020 12:42:54: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 12:42:54: #1 finished! INFO @ Wed, 22 Jul 2020 12:42:54: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:42:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:42:54: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:42:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:42:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:42:54: 14000000 INFO @ Wed, 22 Jul 2020 12:42:55: 11000000 INFO @ Wed, 22 Jul 2020 12:43:04: 15000000 INFO @ Wed, 22 Jul 2020 12:43:04: 12000000 INFO @ Wed, 22 Jul 2020 12:43:13: 16000000 INFO @ Wed, 22 Jul 2020 12:43:14: 13000000 INFO @ Wed, 22 Jul 2020 12:43:22: 17000000 INFO @ Wed, 22 Jul 2020 12:43:24: 14000000 INFO @ Wed, 22 Jul 2020 12:43:31: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:43:31: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:43:31: #1 total tags in treatment: 7870228 INFO @ Wed, 22 Jul 2020 12:43:31: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:43:31: #1 tags after filtering in treatment: 5192828 INFO @ Wed, 22 Jul 2020 12:43:31: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 12:43:31: #1 finished! INFO @ Wed, 22 Jul 2020 12:43:31: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:43:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:43:32: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:43:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:43:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:43:34: 15000000 INFO @ Wed, 22 Jul 2020 12:43:43: 16000000 INFO @ Wed, 22 Jul 2020 12:43:51: 17000000 INFO @ Wed, 22 Jul 2020 12:44:00: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:44:00: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:44:00: #1 total tags in treatment: 7870228 INFO @ Wed, 22 Jul 2020 12:44:00: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:44:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:44:00: #1 tags after filtering in treatment: 5192828 INFO @ Wed, 22 Jul 2020 12:44:00: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 12:44:00: #1 finished! INFO @ Wed, 22 Jul 2020 12:44:00: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:44:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:44:00: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:44:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:44:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911522/SRX6911522.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling