Job ID = 4289469 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,270,348 reads read : 26,270,348 reads written : 26,270,348 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 26270348 reads; of these: 26270348 (100.00%) were unpaired; of these: 4406919 (16.78%) aligned 0 times 19787243 (75.32%) aligned exactly 1 time 2076186 (7.90%) aligned >1 times 83.22% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13111006 / 21863429 = 0.5997 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:56:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:56:36: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:56:36: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:56:46: 1000000 INFO @ Tue, 10 Dec 2019 14:56:56: 2000000 INFO @ Tue, 10 Dec 2019 14:57:05: 3000000 INFO @ Tue, 10 Dec 2019 14:57:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:57:06: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:57:06: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:57:14: 1000000 INFO @ Tue, 10 Dec 2019 14:57:16: 4000000 INFO @ Tue, 10 Dec 2019 14:57:23: 2000000 INFO @ Tue, 10 Dec 2019 14:57:26: 5000000 INFO @ Tue, 10 Dec 2019 14:57:31: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:57:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:57:36: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:57:36: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:57:37: 6000000 INFO @ Tue, 10 Dec 2019 14:57:39: 4000000 INFO @ Tue, 10 Dec 2019 14:57:44: 1000000 INFO @ Tue, 10 Dec 2019 14:57:46: 5000000 INFO @ Tue, 10 Dec 2019 14:57:48: 7000000 INFO @ Tue, 10 Dec 2019 14:57:52: 2000000 INFO @ Tue, 10 Dec 2019 14:57:54: 6000000 INFO @ Tue, 10 Dec 2019 14:57:57: 8000000 INFO @ Tue, 10 Dec 2019 14:57:59: 3000000 INFO @ Tue, 10 Dec 2019 14:58:02: 7000000 INFO @ Tue, 10 Dec 2019 14:58:05: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:58:05: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:58:05: #1 total tags in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:05: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:58:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:58:05: #1 tags after filtering in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:58:05: #1 finished! INFO @ Tue, 10 Dec 2019 14:58:05: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:58:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:58:06: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:58:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:58:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:58:06: 4000000 INFO @ Tue, 10 Dec 2019 14:58:10: 8000000 INFO @ Tue, 10 Dec 2019 14:58:14: 5000000 INFO @ Tue, 10 Dec 2019 14:58:16: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:58:16: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:58:16: #1 total tags in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:16: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:58:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:58:16: #1 tags after filtering in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:58:16: #1 finished! INFO @ Tue, 10 Dec 2019 14:58:16: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:58:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:58:16: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:58:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:58:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10_peaks.narrowPeak: No such file or directory INFO @ Tue, 10 Dec 2019 14:58:21: 6000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:58:28: 7000000 INFO @ Tue, 10 Dec 2019 14:58:35: 8000000 INFO @ Tue, 10 Dec 2019 14:58:41: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:58:41: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:58:41: #1 total tags in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:41: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:58:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:58:41: #1 tags after filtering in treatment: 8752423 INFO @ Tue, 10 Dec 2019 14:58:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:58:41: #1 finished! INFO @ Tue, 10 Dec 2019 14:58:41: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:58:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:58:42: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:58:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:58:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678269/SRX6678269.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。