Job ID = 5791121 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 16,565,112 reads read : 33,130,224 reads written : 33,130,224 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 16565112 reads; of these: 16565112 (100.00%) were paired; of these: 763531 (4.61%) aligned concordantly 0 times 13839834 (83.55%) aligned concordantly exactly 1 time 1961747 (11.84%) aligned concordantly >1 times ---- 763531 pairs aligned concordantly 0 times; of these: 11710 (1.53%) aligned discordantly 1 time ---- 751821 pairs aligned 0 times concordantly or discordantly; of these: 1503642 mates make up the pairs; of these: 1294461 (86.09%) aligned 0 times 169809 (11.29%) aligned exactly 1 time 39372 (2.62%) aligned >1 times 96.09% overall alignment rate Time searching: 00:06:48 Overall time: 00:06:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3015806 / 15807681 = 0.1908 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:51:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:51:41: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:51:41: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:51:45: 1000000 INFO @ Wed, 22 Apr 2020 08:51:50: 2000000 INFO @ Wed, 22 Apr 2020 08:51:54: 3000000 INFO @ Wed, 22 Apr 2020 08:51:59: 4000000 INFO @ Wed, 22 Apr 2020 08:52:03: 5000000 INFO @ Wed, 22 Apr 2020 08:52:08: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:52:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:52:11: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:52:11: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:52:12: 7000000 INFO @ Wed, 22 Apr 2020 08:52:14: 1000000 INFO @ Wed, 22 Apr 2020 08:52:17: 8000000 INFO @ Wed, 22 Apr 2020 08:52:18: 2000000 INFO @ Wed, 22 Apr 2020 08:52:22: 9000000 INFO @ Wed, 22 Apr 2020 08:52:22: 3000000 INFO @ Wed, 22 Apr 2020 08:52:26: 4000000 INFO @ Wed, 22 Apr 2020 08:52:26: 10000000 INFO @ Wed, 22 Apr 2020 08:52:30: 5000000 INFO @ Wed, 22 Apr 2020 08:52:31: 11000000 INFO @ Wed, 22 Apr 2020 08:52:33: 6000000 INFO @ Wed, 22 Apr 2020 08:52:35: 12000000 INFO @ Wed, 22 Apr 2020 08:52:37: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:52:39: 13000000 INFO @ Wed, 22 Apr 2020 08:52:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:52:41: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:52:41: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:52:41: 8000000 INFO @ Wed, 22 Apr 2020 08:52:44: 14000000 INFO @ Wed, 22 Apr 2020 08:52:44: 1000000 INFO @ Wed, 22 Apr 2020 08:52:45: 9000000 INFO @ Wed, 22 Apr 2020 08:52:48: 2000000 INFO @ Wed, 22 Apr 2020 08:52:48: 15000000 INFO @ Wed, 22 Apr 2020 08:52:49: 10000000 INFO @ Wed, 22 Apr 2020 08:52:52: 3000000 INFO @ Wed, 22 Apr 2020 08:52:52: 11000000 INFO @ Wed, 22 Apr 2020 08:52:53: 16000000 INFO @ Wed, 22 Apr 2020 08:52:56: 4000000 INFO @ Wed, 22 Apr 2020 08:52:56: 12000000 INFO @ Wed, 22 Apr 2020 08:52:57: 17000000 INFO @ Wed, 22 Apr 2020 08:53:00: 5000000 INFO @ Wed, 22 Apr 2020 08:53:00: 13000000 INFO @ Wed, 22 Apr 2020 08:53:01: 18000000 INFO @ Wed, 22 Apr 2020 08:53:03: 6000000 INFO @ Wed, 22 Apr 2020 08:53:04: 14000000 INFO @ Wed, 22 Apr 2020 08:53:06: 19000000 INFO @ Wed, 22 Apr 2020 08:53:07: 7000000 INFO @ Wed, 22 Apr 2020 08:53:08: 15000000 INFO @ Wed, 22 Apr 2020 08:53:10: 20000000 INFO @ Wed, 22 Apr 2020 08:53:11: 8000000 INFO @ Wed, 22 Apr 2020 08:53:11: 16000000 INFO @ Wed, 22 Apr 2020 08:53:14: 21000000 INFO @ Wed, 22 Apr 2020 08:53:15: 17000000 INFO @ Wed, 22 Apr 2020 08:53:15: 9000000 INFO @ Wed, 22 Apr 2020 08:53:19: 22000000 INFO @ Wed, 22 Apr 2020 08:53:19: 18000000 INFO @ Wed, 22 Apr 2020 08:53:19: 10000000 INFO @ Wed, 22 Apr 2020 08:53:23: 19000000 INFO @ Wed, 22 Apr 2020 08:53:23: 11000000 INFO @ Wed, 22 Apr 2020 08:53:23: 23000000 INFO @ Wed, 22 Apr 2020 08:53:26: 20000000 INFO @ Wed, 22 Apr 2020 08:53:27: 12000000 INFO @ Wed, 22 Apr 2020 08:53:27: 24000000 INFO @ Wed, 22 Apr 2020 08:53:30: 21000000 INFO @ Wed, 22 Apr 2020 08:53:31: 13000000 INFO @ Wed, 22 Apr 2020 08:53:32: 25000000 INFO @ Wed, 22 Apr 2020 08:53:34: 22000000 INFO @ Wed, 22 Apr 2020 08:53:35: 14000000 INFO @ Wed, 22 Apr 2020 08:53:35: #1 tag size is determined as 37 bps INFO @ Wed, 22 Apr 2020 08:53:35: #1 tag size = 37 INFO @ Wed, 22 Apr 2020 08:53:35: #1 total tags in treatment: 12786117 INFO @ Wed, 22 Apr 2020 08:53:35: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:53:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:53:35: #1 tags after filtering in treatment: 6059574 INFO @ Wed, 22 Apr 2020 08:53:35: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 08:53:35: #1 finished! INFO @ Wed, 22 Apr 2020 08:53:35: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:53:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:53:36: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:53:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:53:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:53:38: 23000000 INFO @ Wed, 22 Apr 2020 08:53:39: 15000000 INFO @ Wed, 22 Apr 2020 08:53:41: 24000000 INFO @ Wed, 22 Apr 2020 08:53:43: 16000000 INFO @ Wed, 22 Apr 2020 08:53:45: 25000000 INFO @ Wed, 22 Apr 2020 08:53:47: 17000000 INFO @ Wed, 22 Apr 2020 08:53:48: #1 tag size is determined as 37 bps INFO @ Wed, 22 Apr 2020 08:53:48: #1 tag size = 37 INFO @ Wed, 22 Apr 2020 08:53:48: #1 total tags in treatment: 12786117 INFO @ Wed, 22 Apr 2020 08:53:48: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:53:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:53:48: #1 tags after filtering in treatment: 6059574 INFO @ Wed, 22 Apr 2020 08:53:48: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 08:53:48: #1 finished! INFO @ Wed, 22 Apr 2020 08:53:48: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:53:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:53:49: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:53:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:53:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:53:50: 18000000 INFO @ Wed, 22 Apr 2020 08:53:54: 19000000 INFO @ Wed, 22 Apr 2020 08:53:58: 20000000 INFO @ Wed, 22 Apr 2020 08:54:02: 21000000 INFO @ Wed, 22 Apr 2020 08:54:05: 22000000 INFO @ Wed, 22 Apr 2020 08:54:09: 23000000 INFO @ Wed, 22 Apr 2020 08:54:13: 24000000 INFO @ Wed, 22 Apr 2020 08:54:17: 25000000 INFO @ Wed, 22 Apr 2020 08:54:20: #1 tag size is determined as 37 bps INFO @ Wed, 22 Apr 2020 08:54:20: #1 tag size = 37 INFO @ Wed, 22 Apr 2020 08:54:20: #1 total tags in treatment: 12786117 INFO @ Wed, 22 Apr 2020 08:54:20: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:54:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:54:21: #1 tags after filtering in treatment: 6059574 INFO @ Wed, 22 Apr 2020 08:54:21: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 08:54:21: #1 finished! INFO @ Wed, 22 Apr 2020 08:54:21: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:54:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:54:21: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:54:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:54:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614917/SRX6614917.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。