Job ID = 2641255 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 13,636,514 reads read : 27,273,028 reads written : 27,273,028 spots read : 13,608,703 reads read : 27,217,406 reads written : 27,217,406 spots read : 14,065,163 reads read : 28,130,326 reads written : 28,130,326 2019-08-24T13:34:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T13:34:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.232' from '172.19.7.41' 2019-08-24T13:34:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.232) from '172.19.7.41' 2019-08-24T13:34:28 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra35/SRR/009415/SRR9641385' 2019-08-24T13:34:28 fasterq-dump.2.9.6 err: cmn_iter.c cmn_get_acc_type( 'SRR9641385', 'SEQUENCE', 'NAME' ).VDBManagerOpenDBRead() -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.232' from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.232) from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra35/SRR/009415/SRR9641385' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_db().VDBManagerOpenDBRead( 'SRR9641385' ) -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.232' from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.232) from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra35/SRR/009415/SRR9641385' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_db().VDBManagerOpenDBRead( 'SRR9641385' ) -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.232' from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.232) from '172.19.7.41' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra35/SRR/009415/SRR9641385' 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_db().VDBManagerOpenDBRead( 'SRR9641385' ) -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) 2019-08-24T13:36:10 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcKrypto,rcFile,rcOpening,rcConnection,rcFailed) spots read : 13,833,347 reads read : 27,666,694 reads written : 27,666,694 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:16 55143727 reads; of these: 55143727 (100.00%) were paired; of these: 18991244 (34.44%) aligned concordantly 0 times 34390190 (62.36%) aligned concordantly exactly 1 time 1762293 (3.20%) aligned concordantly >1 times ---- 18991244 pairs aligned concordantly 0 times; of these: 87486 (0.46%) aligned discordantly 1 time ---- 18903758 pairs aligned 0 times concordantly or discordantly; of these: 37807516 mates make up the pairs; of these: 30513500 (80.71%) aligned 0 times 6907467 (18.27%) aligned exactly 1 time 386549 (1.02%) aligned >1 times 72.33% overall alignment rate Time searching: 00:21:16 Overall time: 00:21:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4500293 / 36238062 = 0.1242 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 23:30:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 23:30:06: #1 read tag files... INFO @ Sat, 24 Aug 2019 23:30:06: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 23:30:12: 1000000 INFO @ Sat, 24 Aug 2019 23:30:18: 2000000 INFO @ Sat, 24 Aug 2019 23:30:23: 3000000 INFO @ Sat, 24 Aug 2019 23:30:29: 4000000 INFO @ Sat, 24 Aug 2019 23:30:35: 5000000 INFO @ Sat, 24 Aug 2019 23:30:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 23:30:36: #1 read tag files... INFO @ Sat, 24 Aug 2019 23:30:36: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 23:30:41: 6000000 INFO @ Sat, 24 Aug 2019 23:30:43: 1000000 INFO @ Sat, 24 Aug 2019 23:30:46: 7000000 INFO @ Sat, 24 Aug 2019 23:30:50: 2000000 INFO @ Sat, 24 Aug 2019 23:30:52: 8000000 INFO @ Sat, 24 Aug 2019 23:30:57: 3000000 INFO @ Sat, 24 Aug 2019 23:30:58: 9000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 23:31:04: 10000000 INFO @ Sat, 24 Aug 2019 23:31:04: 4000000 INFO @ Sat, 24 Aug 2019 23:31:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 23:31:06: #1 read tag files... INFO @ Sat, 24 Aug 2019 23:31:06: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 23:31:10: 11000000 INFO @ Sat, 24 Aug 2019 23:31:11: 5000000 INFO @ Sat, 24 Aug 2019 23:31:14: 1000000 INFO @ Sat, 24 Aug 2019 23:31:15: 12000000 INFO @ Sat, 24 Aug 2019 23:31:18: 6000000 INFO @ Sat, 24 Aug 2019 23:31:21: 13000000 INFO @ Sat, 24 Aug 2019 23:31:22: 2000000 INFO @ Sat, 24 Aug 2019 23:31:24: 7000000 INFO @ Sat, 24 Aug 2019 23:31:27: 14000000 INFO @ Sat, 24 Aug 2019 23:31:30: 3000000 INFO @ Sat, 24 Aug 2019 23:31:31: 8000000 INFO @ Sat, 24 Aug 2019 23:31:33: 15000000 INFO @ Sat, 24 Aug 2019 23:31:38: 9000000 INFO @ Sat, 24 Aug 2019 23:31:38: 4000000 INFO @ Sat, 24 Aug 2019 23:31:39: 16000000 INFO @ Sat, 24 Aug 2019 23:31:44: 10000000 INFO @ Sat, 24 Aug 2019 23:31:45: 17000000 INFO @ Sat, 24 Aug 2019 23:31:46: 5000000 INFO @ Sat, 24 Aug 2019 23:31:51: 11000000 INFO @ Sat, 24 Aug 2019 23:31:51: 18000000 INFO @ Sat, 24 Aug 2019 23:31:54: 6000000 INFO @ Sat, 24 Aug 2019 23:31:57: 12000000 INFO @ Sat, 24 Aug 2019 23:31:57: 19000000 INFO @ Sat, 24 Aug 2019 23:32:01: 7000000 INFO @ Sat, 24 Aug 2019 23:32:04: 20000000 INFO @ Sat, 24 Aug 2019 23:32:04: 13000000 INFO @ Sat, 24 Aug 2019 23:32:09: 8000000 INFO @ Sat, 24 Aug 2019 23:32:10: 21000000 INFO @ Sat, 24 Aug 2019 23:32:10: 14000000 INFO @ Sat, 24 Aug 2019 23:32:16: 22000000 INFO @ Sat, 24 Aug 2019 23:32:17: 9000000 INFO @ Sat, 24 Aug 2019 23:32:17: 15000000 INFO @ Sat, 24 Aug 2019 23:32:22: 23000000 INFO @ Sat, 24 Aug 2019 23:32:24: 16000000 INFO @ Sat, 24 Aug 2019 23:32:24: 10000000 INFO @ Sat, 24 Aug 2019 23:32:28: 24000000 INFO @ Sat, 24 Aug 2019 23:32:30: 17000000 INFO @ Sat, 24 Aug 2019 23:32:32: 11000000 INFO @ Sat, 24 Aug 2019 23:32:35: 25000000 INFO @ Sat, 24 Aug 2019 23:32:37: 18000000 INFO @ Sat, 24 Aug 2019 23:32:40: 12000000 INFO @ Sat, 24 Aug 2019 23:32:41: 26000000 INFO @ Sat, 24 Aug 2019 23:32:44: 19000000 INFO @ Sat, 24 Aug 2019 23:32:47: 27000000 INFO @ Sat, 24 Aug 2019 23:32:47: 13000000 INFO @ Sat, 24 Aug 2019 23:32:51: 20000000 INFO @ Sat, 24 Aug 2019 23:32:53: 28000000 INFO @ Sat, 24 Aug 2019 23:32:55: 14000000 INFO @ Sat, 24 Aug 2019 23:32:57: 21000000 INFO @ Sat, 24 Aug 2019 23:32:59: 29000000 INFO @ Sat, 24 Aug 2019 23:33:03: 15000000 INFO @ Sat, 24 Aug 2019 23:33:04: 22000000 INFO @ Sat, 24 Aug 2019 23:33:06: 30000000 INFO @ Sat, 24 Aug 2019 23:33:10: 16000000 INFO @ Sat, 24 Aug 2019 23:33:11: 23000000 INFO @ Sat, 24 Aug 2019 23:33:12: 31000000 INFO @ Sat, 24 Aug 2019 23:33:17: 24000000 INFO @ Sat, 24 Aug 2019 23:33:18: 32000000 INFO @ Sat, 24 Aug 2019 23:33:18: 17000000 INFO @ Sat, 24 Aug 2019 23:33:24: 33000000 INFO @ Sat, 24 Aug 2019 23:33:24: 25000000 INFO @ Sat, 24 Aug 2019 23:33:26: 18000000 INFO @ Sat, 24 Aug 2019 23:33:30: 34000000 INFO @ Sat, 24 Aug 2019 23:33:31: 26000000 INFO @ Sat, 24 Aug 2019 23:33:33: 19000000 INFO @ Sat, 24 Aug 2019 23:33:36: 35000000 INFO @ Sat, 24 Aug 2019 23:33:37: 27000000 INFO @ Sat, 24 Aug 2019 23:33:41: 20000000 INFO @ Sat, 24 Aug 2019 23:33:43: 36000000 INFO @ Sat, 24 Aug 2019 23:33:44: 28000000 INFO @ Sat, 24 Aug 2019 23:33:49: 37000000 INFO @ Sat, 24 Aug 2019 23:33:49: 21000000 INFO @ Sat, 24 Aug 2019 23:33:51: 29000000 INFO @ Sat, 24 Aug 2019 23:33:55: 38000000 INFO @ Sat, 24 Aug 2019 23:33:56: 22000000 INFO @ Sat, 24 Aug 2019 23:33:57: 30000000 INFO @ Sat, 24 Aug 2019 23:34:01: 39000000 INFO @ Sat, 24 Aug 2019 23:34:04: 31000000 INFO @ Sat, 24 Aug 2019 23:34:04: 23000000 INFO @ Sat, 24 Aug 2019 23:34:07: 40000000 INFO @ Sat, 24 Aug 2019 23:34:10: 32000000 INFO @ Sat, 24 Aug 2019 23:34:12: 24000000 INFO @ Sat, 24 Aug 2019 23:34:13: 41000000 INFO @ Sat, 24 Aug 2019 23:34:17: 33000000 INFO @ Sat, 24 Aug 2019 23:34:19: 42000000 INFO @ Sat, 24 Aug 2019 23:34:19: 25000000 INFO @ Sat, 24 Aug 2019 23:34:23: 34000000 INFO @ Sat, 24 Aug 2019 23:34:25: 43000000 INFO @ Sat, 24 Aug 2019 23:34:27: 26000000 INFO @ Sat, 24 Aug 2019 23:34:30: 35000000 INFO @ Sat, 24 Aug 2019 23:34:31: 44000000 INFO @ Sat, 24 Aug 2019 23:34:35: 27000000 INFO @ Sat, 24 Aug 2019 23:34:36: 36000000 INFO @ Sat, 24 Aug 2019 23:34:37: 45000000 INFO @ Sat, 24 Aug 2019 23:34:42: 28000000 INFO @ Sat, 24 Aug 2019 23:34:43: 37000000 INFO @ Sat, 24 Aug 2019 23:34:43: 46000000 INFO @ Sat, 24 Aug 2019 23:34:49: 47000000 INFO @ Sat, 24 Aug 2019 23:34:49: 38000000 INFO @ Sat, 24 Aug 2019 23:34:50: 29000000 INFO @ Sat, 24 Aug 2019 23:34:55: 48000000 INFO @ Sat, 24 Aug 2019 23:34:55: 39000000 INFO @ Sat, 24 Aug 2019 23:34:58: 30000000 INFO @ Sat, 24 Aug 2019 23:35:01: 49000000 INFO @ Sat, 24 Aug 2019 23:35:02: 40000000 INFO @ Sat, 24 Aug 2019 23:35:05: 31000000 INFO @ Sat, 24 Aug 2019 23:35:07: 50000000 INFO @ Sat, 24 Aug 2019 23:35:08: 41000000 INFO @ Sat, 24 Aug 2019 23:35:13: 51000000 INFO @ Sat, 24 Aug 2019 23:35:13: 32000000 INFO @ Sat, 24 Aug 2019 23:35:14: 42000000 INFO @ Sat, 24 Aug 2019 23:35:19: 52000000 INFO @ Sat, 24 Aug 2019 23:35:21: 43000000 INFO @ Sat, 24 Aug 2019 23:35:21: 33000000 INFO @ Sat, 24 Aug 2019 23:35:25: 53000000 INFO @ Sat, 24 Aug 2019 23:35:27: 44000000 INFO @ Sat, 24 Aug 2019 23:35:28: 34000000 INFO @ Sat, 24 Aug 2019 23:35:31: 54000000 INFO @ Sat, 24 Aug 2019 23:35:33: 45000000 INFO @ Sat, 24 Aug 2019 23:35:36: 35000000 INFO @ Sat, 24 Aug 2019 23:35:37: 55000000 INFO @ Sat, 24 Aug 2019 23:35:40: 46000000 INFO @ Sat, 24 Aug 2019 23:35:43: 56000000 INFO @ Sat, 24 Aug 2019 23:35:43: 36000000 INFO @ Sat, 24 Aug 2019 23:35:46: 47000000 INFO @ Sat, 24 Aug 2019 23:35:49: 57000000 INFO @ Sat, 24 Aug 2019 23:35:51: 37000000 INFO @ Sat, 24 Aug 2019 23:35:52: 48000000 INFO @ Sat, 24 Aug 2019 23:35:55: 58000000 INFO @ Sat, 24 Aug 2019 23:35:58: 38000000 INFO @ Sat, 24 Aug 2019 23:35:59: 49000000 INFO @ Sat, 24 Aug 2019 23:36:01: 59000000 INFO @ Sat, 24 Aug 2019 23:36:05: 50000000 INFO @ Sat, 24 Aug 2019 23:36:05: 39000000 INFO @ Sat, 24 Aug 2019 23:36:07: 60000000 INFO @ Sat, 24 Aug 2019 23:36:11: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 24 Aug 2019 23:36:13: 40000000 INFO @ Sat, 24 Aug 2019 23:36:13: 61000000 INFO @ Sat, 24 Aug 2019 23:36:18: 52000000 INFO @ Sat, 24 Aug 2019 23:36:19: 62000000 INFO @ Sat, 24 Aug 2019 23:36:20: 41000000 INFO @ Sat, 24 Aug 2019 23:36:24: 53000000 INFO @ Sat, 24 Aug 2019 23:36:25: 63000000 INFO @ Sat, 24 Aug 2019 23:36:27: 42000000 INFO @ Sat, 24 Aug 2019 23:36:30: 54000000 INFO @ Sat, 24 Aug 2019 23:36:30: 64000000 BigWig に変換しました。 INFO @ Sat, 24 Aug 2019 23:36:35: 43000000 INFO @ Sat, 24 Aug 2019 23:36:36: 65000000 INFO @ Sat, 24 Aug 2019 23:36:37: 55000000 INFO @ Sat, 24 Aug 2019 23:36:42: 66000000 INFO @ Sat, 24 Aug 2019 23:36:42: 44000000 INFO @ Sat, 24 Aug 2019 23:36:43: 56000000 INFO @ Sat, 24 Aug 2019 23:36:48: 67000000 INFO @ Sat, 24 Aug 2019 23:36:49: 57000000 INFO @ Sat, 24 Aug 2019 23:36:49: 45000000 INFO @ Sat, 24 Aug 2019 23:36:53: 68000000 INFO @ Sat, 24 Aug 2019 23:36:56: 58000000 INFO @ Sat, 24 Aug 2019 23:36:57: 46000000 INFO @ Sat, 24 Aug 2019 23:36:59: 69000000 INFO @ Sat, 24 Aug 2019 23:37:02: 59000000 INFO @ Sat, 24 Aug 2019 23:37:04: 47000000 INFO @ Sat, 24 Aug 2019 23:37:05: 70000000 INFO @ Sat, 24 Aug 2019 23:37:08: 60000000 INFO @ Sat, 24 Aug 2019 23:37:10: #1 tag size is determined as 35 bps INFO @ Sat, 24 Aug 2019 23:37:10: #1 tag size = 35 INFO @ Sat, 24 Aug 2019 23:37:10: #1 total tags in treatment: 31656049 INFO @ Sat, 24 Aug 2019 23:37:10: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 23:37:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 23:37:10: #1 tags after filtering in treatment: 11366390 INFO @ Sat, 24 Aug 2019 23:37:10: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 24 Aug 2019 23:37:10: #1 finished! INFO @ Sat, 24 Aug 2019 23:37:10: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 23:37:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 23:37:11: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 23:37:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 23:37:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 23:37:11: 48000000 INFO @ Sat, 24 Aug 2019 23:37:14: 61000000 INFO @ Sat, 24 Aug 2019 23:37:19: 49000000 INFO @ Sat, 24 Aug 2019 23:37:21: 62000000 INFO @ Sat, 24 Aug 2019 23:37:26: 50000000 INFO @ Sat, 24 Aug 2019 23:37:27: 63000000 INFO @ Sat, 24 Aug 2019 23:37:33: 64000000 INFO @ Sat, 24 Aug 2019 23:37:33: 51000000 INFO @ Sat, 24 Aug 2019 23:37:39: 65000000 INFO @ Sat, 24 Aug 2019 23:37:41: 52000000 INFO @ Sat, 24 Aug 2019 23:37:46: 66000000 INFO @ Sat, 24 Aug 2019 23:37:48: 53000000 INFO @ Sat, 24 Aug 2019 23:37:52: 67000000 INFO @ Sat, 24 Aug 2019 23:37:55: 54000000 INFO @ Sat, 24 Aug 2019 23:37:58: 68000000 INFO @ Sat, 24 Aug 2019 23:38:03: 55000000 INFO @ Sat, 24 Aug 2019 23:38:04: 69000000 INFO @ Sat, 24 Aug 2019 23:38:10: 56000000 INFO @ Sat, 24 Aug 2019 23:38:11: 70000000 INFO @ Sat, 24 Aug 2019 23:38:16: #1 tag size is determined as 35 bps INFO @ Sat, 24 Aug 2019 23:38:16: #1 tag size = 35 INFO @ Sat, 24 Aug 2019 23:38:16: #1 total tags in treatment: 31656049 INFO @ Sat, 24 Aug 2019 23:38:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 23:38:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 23:38:17: #1 tags after filtering in treatment: 11366390 INFO @ Sat, 24 Aug 2019 23:38:17: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 24 Aug 2019 23:38:17: #1 finished! INFO @ Sat, 24 Aug 2019 23:38:17: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 23:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 23:38:17: 57000000 INFO @ Sat, 24 Aug 2019 23:38:18: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 23:38:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 23:38:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 23:38:24: 58000000 INFO @ Sat, 24 Aug 2019 23:38:31: 59000000 INFO @ Sat, 24 Aug 2019 23:38:38: 60000000 INFO @ Sat, 24 Aug 2019 23:38:45: 61000000 INFO @ Sat, 24 Aug 2019 23:38:53: 62000000 INFO @ Sat, 24 Aug 2019 23:39:00: 63000000 INFO @ Sat, 24 Aug 2019 23:39:07: 64000000 INFO @ Sat, 24 Aug 2019 23:39:14: 65000000 INFO @ Sat, 24 Aug 2019 23:39:21: 66000000 INFO @ Sat, 24 Aug 2019 23:39:28: 67000000 INFO @ Sat, 24 Aug 2019 23:39:35: 68000000 INFO @ Sat, 24 Aug 2019 23:39:42: 69000000 INFO @ Sat, 24 Aug 2019 23:39:49: 70000000 INFO @ Sat, 24 Aug 2019 23:39:55: #1 tag size is determined as 35 bps INFO @ Sat, 24 Aug 2019 23:39:55: #1 tag size = 35 INFO @ Sat, 24 Aug 2019 23:39:55: #1 total tags in treatment: 31656049 INFO @ Sat, 24 Aug 2019 23:39:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 23:39:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 23:39:56: #1 tags after filtering in treatment: 11366390 INFO @ Sat, 24 Aug 2019 23:39:56: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 24 Aug 2019 23:39:56: #1 finished! INFO @ Sat, 24 Aug 2019 23:39:56: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 23:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 23:39:57: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 23:39:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 23:39:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6403231/SRX6403231.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling