Job ID = 11245102 sra ファイルのダウンロード中... Completed: 362800K bytes transferred in 6 seconds (468291K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 19999456 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4668658/SRR7817176.sra Written 19999456 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4668658/SRR7817176.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:36 19999456 reads; of these: 19999456 (100.00%) were unpaired; of these: 2629379 (13.15%) aligned 0 times 15690820 (78.46%) aligned exactly 1 time 1679257 (8.40%) aligned >1 times 86.85% overall alignment rate Time searching: 00:03:36 Overall time: 00:03:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8888580 / 17370077 = 0.5117 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:30:58: # Command line: callpeak -t SRX4668658.bam -f BAM -g 12100000 -n SRX4668658.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4668658.05 # format = BAM # ChIP-seq file = ['SRX4668658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:30:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:30:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:30:58: # Command line: callpeak -t SRX4668658.bam -f BAM -g 12100000 -n SRX4668658.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4668658.20 # format = BAM # ChIP-seq file = ['SRX4668658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:30:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:30:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:30:58: # Command line: callpeak -t SRX4668658.bam -f BAM -g 12100000 -n SRX4668658.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4668658.10 # format = BAM # ChIP-seq file = ['SRX4668658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:30:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:30:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:31:04: 1000000 INFO @ Tue, 09 Oct 2018 23:31:05: 1000000 INFO @ Tue, 09 Oct 2018 23:31:05: 1000000 INFO @ Tue, 09 Oct 2018 23:31:11: 2000000 INFO @ Tue, 09 Oct 2018 23:31:11: 2000000 INFO @ Tue, 09 Oct 2018 23:31:11: 2000000 INFO @ Tue, 09 Oct 2018 23:31:17: 3000000 INFO @ Tue, 09 Oct 2018 23:31:18: 3000000 INFO @ Tue, 09 Oct 2018 23:31:18: 3000000 INFO @ Tue, 09 Oct 2018 23:31:24: 4000000 INFO @ Tue, 09 Oct 2018 23:31:25: 4000000 INFO @ Tue, 09 Oct 2018 23:31:26: 4000000 INFO @ Tue, 09 Oct 2018 23:31:31: 5000000 INFO @ Tue, 09 Oct 2018 23:31:32: 5000000 INFO @ Tue, 09 Oct 2018 23:31:33: 5000000 INFO @ Tue, 09 Oct 2018 23:31:37: 6000000 INFO @ Tue, 09 Oct 2018 23:31:39: 6000000 INFO @ Tue, 09 Oct 2018 23:31:40: 6000000 INFO @ Tue, 09 Oct 2018 23:31:44: 7000000 INFO @ Tue, 09 Oct 2018 23:31:45: 7000000 INFO @ Tue, 09 Oct 2018 23:31:47: 7000000 INFO @ Tue, 09 Oct 2018 23:31:51: 8000000 INFO @ Tue, 09 Oct 2018 23:31:52: 8000000 INFO @ Tue, 09 Oct 2018 23:31:54: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:31:54: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:31:54: #1 total tags in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:54: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:31:54: #1 tags after filtering in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:31:54: #1 finished! INFO @ Tue, 09 Oct 2018 23:31:54: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:31:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:31:54: 8000000 INFO @ Tue, 09 Oct 2018 23:31:55: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:31:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:31:55: Process for pairing-model is terminated! cat: SRX4668658.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4668658.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:31:55: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:31:55: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:31:55: #1 total tags in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:55: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:31:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:31:55: #1 tags after filtering in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:31:55: #1 finished! INFO @ Tue, 09 Oct 2018 23:31:55: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:31:56: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:31:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:31:56: Process for pairing-model is terminated! cat: SRX4668658.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4668658.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:31:57: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:31:57: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:31:57: #1 total tags in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:57: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:31:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:31:57: #1 tags after filtering in treatment: 8481497 INFO @ Tue, 09 Oct 2018 23:31:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:31:57: #1 finished! INFO @ Tue, 09 Oct 2018 23:31:57: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:31:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:31:58: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:31:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:31:58: Process for pairing-model is terminated! cat: SRX4668658.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4668658.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4668658.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。