Job ID = 14521860 SRX = SRX4623301 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14698314 spots for SRR7767706/SRR7767706.sra Written 14698314 spots for SRR7767706/SRR7767706.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:38 14698314 reads; of these: 14698314 (100.00%) were paired; of these: 1901340 (12.94%) aligned concordantly 0 times 11248712 (76.53%) aligned concordantly exactly 1 time 1548262 (10.53%) aligned concordantly >1 times ---- 1901340 pairs aligned concordantly 0 times; of these: 990470 (52.09%) aligned discordantly 1 time ---- 910870 pairs aligned 0 times concordantly or discordantly; of these: 1821740 mates make up the pairs; of these: 1189861 (65.31%) aligned 0 times 298233 (16.37%) aligned exactly 1 time 333646 (18.31%) aligned >1 times 95.95% overall alignment rate Time searching: 00:07:38 Overall time: 00:07:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 169715 / 13744137 = 0.0123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:59:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:59:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:59:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:59:25: 1000000 INFO @ Sat, 15 Jan 2022 21:59:29: 2000000 INFO @ Sat, 15 Jan 2022 21:59:34: 3000000 INFO @ Sat, 15 Jan 2022 21:59:38: 4000000 INFO @ Sat, 15 Jan 2022 21:59:42: 5000000 INFO @ Sat, 15 Jan 2022 21:59:47: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:59:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:59:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:59:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:59:51: 7000000 INFO @ Sat, 15 Jan 2022 21:59:55: 1000000 INFO @ Sat, 15 Jan 2022 21:59:55: 8000000 INFO @ Sat, 15 Jan 2022 21:59:59: 2000000 INFO @ Sat, 15 Jan 2022 21:59:59: 9000000 INFO @ Sat, 15 Jan 2022 22:00:04: 3000000 INFO @ Sat, 15 Jan 2022 22:00:04: 10000000 INFO @ Sat, 15 Jan 2022 22:00:08: 4000000 INFO @ Sat, 15 Jan 2022 22:00:08: 11000000 INFO @ Sat, 15 Jan 2022 22:00:13: 5000000 INFO @ Sat, 15 Jan 2022 22:00:13: 12000000 INFO @ Sat, 15 Jan 2022 22:00:17: 6000000 INFO @ Sat, 15 Jan 2022 22:00:17: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:00:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:00:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:00:21: 7000000 INFO @ Sat, 15 Jan 2022 22:00:22: 14000000 INFO @ Sat, 15 Jan 2022 22:00:25: 1000000 INFO @ Sat, 15 Jan 2022 22:00:26: 8000000 INFO @ Sat, 15 Jan 2022 22:00:26: 15000000 INFO @ Sat, 15 Jan 2022 22:00:30: 2000000 INFO @ Sat, 15 Jan 2022 22:00:31: 9000000 INFO @ Sat, 15 Jan 2022 22:00:31: 16000000 INFO @ Sat, 15 Jan 2022 22:00:35: 3000000 INFO @ Sat, 15 Jan 2022 22:00:35: 17000000 INFO @ Sat, 15 Jan 2022 22:00:35: 10000000 INFO @ Sat, 15 Jan 2022 22:00:40: 18000000 INFO @ Sat, 15 Jan 2022 22:00:40: 11000000 INFO @ Sat, 15 Jan 2022 22:00:40: 4000000 INFO @ Sat, 15 Jan 2022 22:00:44: 19000000 INFO @ Sat, 15 Jan 2022 22:00:44: 12000000 INFO @ Sat, 15 Jan 2022 22:00:44: 5000000 INFO @ Sat, 15 Jan 2022 22:00:49: 20000000 INFO @ Sat, 15 Jan 2022 22:00:49: 13000000 INFO @ Sat, 15 Jan 2022 22:00:49: 6000000 INFO @ Sat, 15 Jan 2022 22:00:54: 21000000 INFO @ Sat, 15 Jan 2022 22:00:54: 7000000 INFO @ Sat, 15 Jan 2022 22:00:54: 14000000 INFO @ Sat, 15 Jan 2022 22:00:58: 22000000 INFO @ Sat, 15 Jan 2022 22:00:58: 8000000 INFO @ Sat, 15 Jan 2022 22:00:58: 15000000 INFO @ Sat, 15 Jan 2022 22:01:03: 23000000 INFO @ Sat, 15 Jan 2022 22:01:03: 9000000 INFO @ Sat, 15 Jan 2022 22:01:03: 16000000 INFO @ Sat, 15 Jan 2022 22:01:07: 24000000 INFO @ Sat, 15 Jan 2022 22:01:08: 17000000 INFO @ Sat, 15 Jan 2022 22:01:08: 10000000 INFO @ Sat, 15 Jan 2022 22:01:12: 25000000 INFO @ Sat, 15 Jan 2022 22:01:12: 18000000 INFO @ Sat, 15 Jan 2022 22:01:13: 11000000 INFO @ Sat, 15 Jan 2022 22:01:16: 26000000 INFO @ Sat, 15 Jan 2022 22:01:17: 19000000 INFO @ Sat, 15 Jan 2022 22:01:18: 12000000 INFO @ Sat, 15 Jan 2022 22:01:21: 27000000 INFO @ Sat, 15 Jan 2022 22:01:22: 20000000 INFO @ Sat, 15 Jan 2022 22:01:22: 13000000 INFO @ Sat, 15 Jan 2022 22:01:25: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:01:25: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:01:25: #1 total tags in treatment: 12630641 INFO @ Sat, 15 Jan 2022 22:01:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:25: #1 tags after filtering in treatment: 9004849 INFO @ Sat, 15 Jan 2022 22:01:25: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 22:01:25: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 22:01:26: 21000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:01:27: 14000000 INFO @ Sat, 15 Jan 2022 22:01:31: 22000000 INFO @ Sat, 15 Jan 2022 22:01:31: 15000000 INFO @ Sat, 15 Jan 2022 22:01:35: 23000000 INFO @ Sat, 15 Jan 2022 22:01:36: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:01:40: 24000000 INFO @ Sat, 15 Jan 2022 22:01:41: 17000000 INFO @ Sat, 15 Jan 2022 22:01:44: 25000000 INFO @ Sat, 15 Jan 2022 22:01:45: 18000000 INFO @ Sat, 15 Jan 2022 22:01:49: 26000000 INFO @ Sat, 15 Jan 2022 22:01:50: 19000000 INFO @ Sat, 15 Jan 2022 22:01:54: 27000000 INFO @ Sat, 15 Jan 2022 22:01:54: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:01:58: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:01:58: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:01:58: #1 total tags in treatment: 12630641 INFO @ Sat, 15 Jan 2022 22:01:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:58: #1 tags after filtering in treatment: 9004849 INFO @ Sat, 15 Jan 2022 22:01:58: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 22:01:58: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:59: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 22:01:59: 21000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:02:03: 22000000 INFO @ Sat, 15 Jan 2022 22:02:07: 23000000 INFO @ Sat, 15 Jan 2022 22:02:12: 24000000 INFO @ Sat, 15 Jan 2022 22:02:16: 25000000 INFO @ Sat, 15 Jan 2022 22:02:20: 26000000 INFO @ Sat, 15 Jan 2022 22:02:25: 27000000 INFO @ Sat, 15 Jan 2022 22:02:29: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 22:02:29: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 22:02:29: #1 total tags in treatment: 12630641 INFO @ Sat, 15 Jan 2022 22:02:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:02:29: #1 tags after filtering in treatment: 9004849 INFO @ Sat, 15 Jan 2022 22:02:29: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 22:02:29: #1 finished! INFO @ Sat, 15 Jan 2022 22:02:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:02:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:02:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:02:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:02:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623301/SRX4623301.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling