Job ID = 11634674 sra ファイルのダウンロード中... Completed: 747089K bytes transferred in 9 seconds (632409K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 35552339 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092954/SRR7175382.sra Written 35552339 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092954/SRR7175382.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:00 35552339 reads; of these: 35552339 (100.00%) were unpaired; of these: 1606046 (4.52%) aligned 0 times 26143432 (73.54%) aligned exactly 1 time 7802861 (21.95%) aligned >1 times 95.48% overall alignment rate Time searching: 00:06:00 Overall time: 00:06:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18617343 / 33946293 = 0.5484 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:36:29: # Command line: callpeak -t SRX4092954.bam -f BAM -g 12100000 -n SRX4092954.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4092954.20 # format = BAM # ChIP-seq file = ['SRX4092954.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:29: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:29: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:36:29: # Command line: callpeak -t SRX4092954.bam -f BAM -g 12100000 -n SRX4092954.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4092954.05 # format = BAM # ChIP-seq file = ['SRX4092954.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:29: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:29: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:36:29: # Command line: callpeak -t SRX4092954.bam -f BAM -g 12100000 -n SRX4092954.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4092954.10 # format = BAM # ChIP-seq file = ['SRX4092954.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:36:29: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:36:29: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:36:36: 1000000 INFO @ Fri, 15 Feb 2019 10:36:36: 1000000 INFO @ Fri, 15 Feb 2019 10:36:36: 1000000 INFO @ Fri, 15 Feb 2019 10:36:43: 2000000 INFO @ Fri, 15 Feb 2019 10:36:43: 2000000 INFO @ Fri, 15 Feb 2019 10:36:43: 2000000 INFO @ Fri, 15 Feb 2019 10:36:49: 3000000 INFO @ Fri, 15 Feb 2019 10:36:50: 3000000 INFO @ Fri, 15 Feb 2019 10:36:50: 3000000 INFO @ Fri, 15 Feb 2019 10:36:56: 4000000 INFO @ Fri, 15 Feb 2019 10:36:56: 4000000 INFO @ Fri, 15 Feb 2019 10:36:57: 4000000 INFO @ Fri, 15 Feb 2019 10:37:03: 5000000 INFO @ Fri, 15 Feb 2019 10:37:03: 5000000 INFO @ Fri, 15 Feb 2019 10:37:03: 5000000 INFO @ Fri, 15 Feb 2019 10:37:10: 6000000 INFO @ Fri, 15 Feb 2019 10:37:10: 6000000 INFO @ Fri, 15 Feb 2019 10:37:10: 6000000 INFO @ Fri, 15 Feb 2019 10:37:16: 7000000 INFO @ Fri, 15 Feb 2019 10:37:17: 7000000 INFO @ Fri, 15 Feb 2019 10:37:17: 7000000 INFO @ Fri, 15 Feb 2019 10:37:23: 8000000 INFO @ Fri, 15 Feb 2019 10:37:24: 8000000 INFO @ Fri, 15 Feb 2019 10:37:24: 8000000 INFO @ Fri, 15 Feb 2019 10:37:30: 9000000 INFO @ Fri, 15 Feb 2019 10:37:31: 9000000 INFO @ Fri, 15 Feb 2019 10:37:31: 9000000 INFO @ Fri, 15 Feb 2019 10:37:37: 10000000 INFO @ Fri, 15 Feb 2019 10:37:38: 10000000 INFO @ Fri, 15 Feb 2019 10:37:39: 10000000 INFO @ Fri, 15 Feb 2019 10:37:43: 11000000 INFO @ Fri, 15 Feb 2019 10:37:45: 11000000 INFO @ Fri, 15 Feb 2019 10:37:46: 11000000 INFO @ Fri, 15 Feb 2019 10:37:50: 12000000 INFO @ Fri, 15 Feb 2019 10:37:52: 12000000 INFO @ Fri, 15 Feb 2019 10:37:53: 12000000 INFO @ Fri, 15 Feb 2019 10:37:57: 13000000 INFO @ Fri, 15 Feb 2019 10:37:59: 13000000 INFO @ Fri, 15 Feb 2019 10:37:59: 13000000 INFO @ Fri, 15 Feb 2019 10:38:04: 14000000 INFO @ Fri, 15 Feb 2019 10:38:06: 14000000 INFO @ Fri, 15 Feb 2019 10:38:06: 14000000 INFO @ Fri, 15 Feb 2019 10:38:10: 15000000 INFO @ Fri, 15 Feb 2019 10:38:13: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:13: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:13: #1 total tags in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:13: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:13: 15000000 INFO @ Fri, 15 Feb 2019 10:38:13: #1 tags after filtering in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:13: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:13: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:13: 15000000 INFO @ Fri, 15 Feb 2019 10:38:14: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:14: Process for pairing-model is terminated! cat: SRX4092954.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092954.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:38:15: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:15: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:15: #1 total tags in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:15: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:15: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:15: #1 total tags in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:15: #1 tags after filtering in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:15: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:15: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:16: #1 tags after filtering in treatment: 15328950 INFO @ Fri, 15 Feb 2019 10:38:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:16: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:16: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:16: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:16: Process for pairing-model is terminated! cat: SRX4092954.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092954.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:38:16: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:16: Process for pairing-model is terminated! cat: SRX4092954.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092954.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092954.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。