Job ID = 11162569 sra ファイルのダウンロード中... Completed: 698794K bytes transferred in 12 seconds (472932K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 20930939 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585183/SRR6495883.sra Written 20930939 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585183/SRR6495883.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 20930939 reads; of these: 20930939 (100.00%) were unpaired; of these: 2974602 (14.21%) aligned 0 times 15933458 (76.12%) aligned exactly 1 time 2022879 (9.66%) aligned >1 times 85.79% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7770395 / 17956337 = 0.4327 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:10:08: # Command line: callpeak -t SRX3585183.bam -f BAM -g 12100000 -n SRX3585183.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3585183.20 # format = BAM # ChIP-seq file = ['SRX3585183.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:10:08: # Command line: callpeak -t SRX3585183.bam -f BAM -g 12100000 -n SRX3585183.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3585183.05 # format = BAM # ChIP-seq file = ['SRX3585183.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:10:08: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:10:08: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:10:08: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:10:08: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:10:08: # Command line: callpeak -t SRX3585183.bam -f BAM -g 12100000 -n SRX3585183.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3585183.10 # format = BAM # ChIP-seq file = ['SRX3585183.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:10:08: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:10:08: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:10:17: 1000000 INFO @ Wed, 05 Sep 2018 10:10:17: 1000000 INFO @ Wed, 05 Sep 2018 10:10:17: 1000000 INFO @ Wed, 05 Sep 2018 10:10:25: 2000000 INFO @ Wed, 05 Sep 2018 10:10:25: 2000000 INFO @ Wed, 05 Sep 2018 10:10:25: 2000000 INFO @ Wed, 05 Sep 2018 10:10:34: 3000000 INFO @ Wed, 05 Sep 2018 10:10:34: 3000000 INFO @ Wed, 05 Sep 2018 10:10:34: 3000000 INFO @ Wed, 05 Sep 2018 10:10:42: 4000000 INFO @ Wed, 05 Sep 2018 10:10:42: 4000000 INFO @ Wed, 05 Sep 2018 10:10:42: 4000000 INFO @ Wed, 05 Sep 2018 10:10:51: 5000000 INFO @ Wed, 05 Sep 2018 10:10:51: 5000000 INFO @ Wed, 05 Sep 2018 10:10:51: 5000000 INFO @ Wed, 05 Sep 2018 10:10:59: 6000000 INFO @ Wed, 05 Sep 2018 10:10:59: 6000000 INFO @ Wed, 05 Sep 2018 10:10:59: 6000000 INFO @ Wed, 05 Sep 2018 10:11:08: 7000000 INFO @ Wed, 05 Sep 2018 10:11:08: 7000000 INFO @ Wed, 05 Sep 2018 10:11:08: 7000000 INFO @ Wed, 05 Sep 2018 10:11:17: 8000000 INFO @ Wed, 05 Sep 2018 10:11:17: 8000000 INFO @ Wed, 05 Sep 2018 10:11:17: 8000000 INFO @ Wed, 05 Sep 2018 10:11:25: 9000000 INFO @ Wed, 05 Sep 2018 10:11:25: 9000000 INFO @ Wed, 05 Sep 2018 10:11:25: 9000000 INFO @ Wed, 05 Sep 2018 10:11:34: 10000000 INFO @ Wed, 05 Sep 2018 10:11:34: 10000000 INFO @ Wed, 05 Sep 2018 10:11:34: 10000000 INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:11:36: #1 total tags in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:11:36: #1 total tags in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:11:36: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:11:36: #1 total tags in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:11:36: #1 tags after filtering in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:11:36: #1 finished! INFO @ Wed, 05 Sep 2018 10:11:36: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:11:36: #1 tags after filtering in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:11:36: #1 finished! INFO @ Wed, 05 Sep 2018 10:11:36: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:11:36: #1 tags after filtering in treatment: 10185942 INFO @ Wed, 05 Sep 2018 10:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:11:36: #1 finished! INFO @ Wed, 05 Sep 2018 10:11:36: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:11:37: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:11:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:11:37: Process for pairing-model is terminated! INFO @ Wed, 05 Sep 2018 10:11:37: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:11:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:11:37: Process for pairing-model is terminated! INFO @ Wed, 05 Sep 2018 10:11:37: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:11:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:11:37: Process for pairing-model is terminated! cat: SRX3585183.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3585183.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3585183.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585183.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.20_*.xls': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis pass1 - making usageList (0 chroms): 4 millis rm: cannot remove `SRX3585183.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `SRX3585183.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585183.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。