Job ID = 2010165 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,676,806 reads read : 18,676,806 reads written : 18,676,806 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:33 18676806 reads; of these: 18676806 (100.00%) were unpaired; of these: 332209 (1.78%) aligned 0 times 15905856 (85.16%) aligned exactly 1 time 2438741 (13.06%) aligned >1 times 98.22% overall alignment rate Time searching: 00:03:33 Overall time: 00:03:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8894666 / 18344597 = 0.4849 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:55:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:55:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:55:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:55:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:55:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:55:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:55:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:55:36: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:55:36: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:55:41: 1000000 INFO @ Fri, 05 Jul 2019 21:55:43: 1000000 INFO @ Fri, 05 Jul 2019 21:55:43: 1000000 INFO @ Fri, 05 Jul 2019 21:55:48: 2000000 INFO @ Fri, 05 Jul 2019 21:55:50: 2000000 INFO @ Fri, 05 Jul 2019 21:55:50: 2000000 INFO @ Fri, 05 Jul 2019 21:55:55: 3000000 INFO @ Fri, 05 Jul 2019 21:55:56: 3000000 INFO @ Fri, 05 Jul 2019 21:55:57: 3000000 INFO @ Fri, 05 Jul 2019 21:56:02: 4000000 INFO @ Fri, 05 Jul 2019 21:56:03: 4000000 INFO @ Fri, 05 Jul 2019 21:56:04: 4000000 INFO @ Fri, 05 Jul 2019 21:56:09: 5000000 INFO @ Fri, 05 Jul 2019 21:56:10: 5000000 INFO @ Fri, 05 Jul 2019 21:56:11: 5000000 INFO @ Fri, 05 Jul 2019 21:56:16: 6000000 INFO @ Fri, 05 Jul 2019 21:56:17: 6000000 INFO @ Fri, 05 Jul 2019 21:56:19: 6000000 INFO @ Fri, 05 Jul 2019 21:56:22: 7000000 INFO @ Fri, 05 Jul 2019 21:56:24: 7000000 INFO @ Fri, 05 Jul 2019 21:56:26: 7000000 INFO @ Fri, 05 Jul 2019 21:56:29: 8000000 INFO @ Fri, 05 Jul 2019 21:56:31: 8000000 INFO @ Fri, 05 Jul 2019 21:56:33: 8000000 INFO @ Fri, 05 Jul 2019 21:56:36: 9000000 INFO @ Fri, 05 Jul 2019 21:56:38: 9000000 INFO @ Fri, 05 Jul 2019 21:56:39: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:56:39: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:56:39: #1 total tags in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:39: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:56:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:56:39: #1 tags after filtering in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:56:39: #1 finished! INFO @ Fri, 05 Jul 2019 21:56:39: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:56:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:56:40: 9000000 INFO @ Fri, 05 Jul 2019 21:56:40: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:56:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:56:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:56:41: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:56:41: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:56:41: #1 total tags in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:41: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:56:41: #1 tags after filtering in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:56:41: #1 finished! INFO @ Fri, 05 Jul 2019 21:56:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:56:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:56:42: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:56:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:56:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:56:43: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:56:43: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:56:43: #1 total tags in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:56:43: #1 tags after filtering in treatment: 9449931 INFO @ Fri, 05 Jul 2019 21:56:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:56:43: #1 finished! INFO @ Fri, 05 Jul 2019 21:56:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:56:44: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:56:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:56:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894834/SRX2894834.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。