Job ID = 7098146 SRX = SRX2797010 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7522655 spots for SRR5527824/SRR5527824.sra Written 7522655 spots for SRR5527824/SRR5527824.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:15 7522655 reads; of these: 7522655 (100.00%) were unpaired; of these: 85574 (1.14%) aligned 0 times 6064471 (80.62%) aligned exactly 1 time 1372610 (18.25%) aligned >1 times 98.86% overall alignment rate Time searching: 00:01:15 Overall time: 00:01:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3042149 / 7437081 = 0.4091 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:54:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:54:32: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:54:32: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:54:38: 1000000 INFO @ Wed, 22 Jul 2020 11:54:43: 2000000 INFO @ Wed, 22 Jul 2020 11:54:49: 3000000 INFO @ Wed, 22 Jul 2020 11:54:55: 4000000 INFO @ Wed, 22 Jul 2020 11:54:57: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:54:57: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:54:57: #1 total tags in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:54:57: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:54:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:54:57: #1 tags after filtering in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:54:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:54:57: #1 finished! INFO @ Wed, 22 Jul 2020 11:54:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:54:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:54:57: #2 number of paired peaks: 28 WARNING @ Wed, 22 Jul 2020 11:54:57: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:54:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:55:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:55:02: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:55:02: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:55:08: 1000000 INFO @ Wed, 22 Jul 2020 11:55:14: 2000000 INFO @ Wed, 22 Jul 2020 11:55:19: 3000000 INFO @ Wed, 22 Jul 2020 11:55:25: 4000000 INFO @ Wed, 22 Jul 2020 11:55:27: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:55:27: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:55:27: #1 total tags in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:55:27: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:55:27: #1 tags after filtering in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:55:27: #1 finished! INFO @ Wed, 22 Jul 2020 11:55:27: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:55:28: #2 number of paired peaks: 28 WARNING @ Wed, 22 Jul 2020 11:55:28: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:55:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:55:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:55:33: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:55:33: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:55:38: 1000000 INFO @ Wed, 22 Jul 2020 11:55:44: 2000000 INFO @ Wed, 22 Jul 2020 11:55:50: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:55:56: 4000000 INFO @ Wed, 22 Jul 2020 11:55:58: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:55:58: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:55:58: #1 total tags in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:55:58: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:55:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:55:58: #1 tags after filtering in treatment: 4394932 INFO @ Wed, 22 Jul 2020 11:55:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:55:58: #1 finished! INFO @ Wed, 22 Jul 2020 11:55:58: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:55:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:55:59: #2 number of paired peaks: 28 WARNING @ Wed, 22 Jul 2020 11:55:59: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:55:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797010/SRX2797010.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。