Job ID = 9160128 sra ファイルのダウンロード中... Completed: 467933K bytes transferred in 8 seconds (457584K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20960381 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2619072/SRR5319657.sra Written 20960381 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 20960381 reads; of these: 20960381 (100.00%) were unpaired; of these: 445198 (2.12%) aligned 0 times 17677068 (84.34%) aligned exactly 1 time 2838115 (13.54%) aligned >1 times 97.88% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8984840 / 20515183 = 0.4380 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 02:03:17: # Command line: callpeak -t SRX2619072.bam -f BAM -g 12100000 -n SRX2619072.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2619072.20 # format = BAM # ChIP-seq file = ['SRX2619072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:17: # Command line: callpeak -t SRX2619072.bam -f BAM -g 12100000 -n SRX2619072.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2619072.10 # format = BAM # ChIP-seq file = ['SRX2619072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:17: # Command line: callpeak -t SRX2619072.bam -f BAM -g 12100000 -n SRX2619072.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2619072.05 # format = BAM # ChIP-seq file = ['SRX2619072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:24: 1000000 INFO @ Wed, 28 Jun 2017 02:03:24: 1000000 INFO @ Wed, 28 Jun 2017 02:03:25: 1000000 INFO @ Wed, 28 Jun 2017 02:03:32: 2000000 INFO @ Wed, 28 Jun 2017 02:03:32: 2000000 INFO @ Wed, 28 Jun 2017 02:03:33: 2000000 INFO @ Wed, 28 Jun 2017 02:03:39: 3000000 INFO @ Wed, 28 Jun 2017 02:03:39: 3000000 INFO @ Wed, 28 Jun 2017 02:03:42: 3000000 INFO @ Wed, 28 Jun 2017 02:03:46: 4000000 INFO @ Wed, 28 Jun 2017 02:03:46: 4000000 INFO @ Wed, 28 Jun 2017 02:03:50: 4000000 INFO @ Wed, 28 Jun 2017 02:03:53: 5000000 INFO @ Wed, 28 Jun 2017 02:03:53: 5000000 INFO @ Wed, 28 Jun 2017 02:03:58: 5000000 INFO @ Wed, 28 Jun 2017 02:04:01: 6000000 INFO @ Wed, 28 Jun 2017 02:04:01: 6000000 INFO @ Wed, 28 Jun 2017 02:04:06: 6000000 INFO @ Wed, 28 Jun 2017 02:04:08: 7000000 INFO @ Wed, 28 Jun 2017 02:04:08: 7000000 INFO @ Wed, 28 Jun 2017 02:04:15: 7000000 INFO @ Wed, 28 Jun 2017 02:04:16: 8000000 INFO @ Wed, 28 Jun 2017 02:04:16: 8000000 INFO @ Wed, 28 Jun 2017 02:04:23: 8000000 INFO @ Wed, 28 Jun 2017 02:04:23: 9000000 INFO @ Wed, 28 Jun 2017 02:04:23: 9000000 INFO @ Wed, 28 Jun 2017 02:04:30: 9000000 INFO @ Wed, 28 Jun 2017 02:04:31: 10000000 INFO @ Wed, 28 Jun 2017 02:04:31: 10000000 INFO @ Wed, 28 Jun 2017 02:04:38: 10000000 INFO @ Wed, 28 Jun 2017 02:04:38: 11000000 INFO @ Wed, 28 Jun 2017 02:04:38: 11000000 INFO @ Wed, 28 Jun 2017 02:04:41: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:41: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:41: #1 total tags in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:41: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:41: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:41: #1 total tags in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:42: #1 tags after filtering in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:42: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:42: #1 tags after filtering in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:42: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:42: Process for pairing-model is terminated! cat: SRX2619072.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619072.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:04:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:42: Process for pairing-model is terminated! cat: SRX2619072.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619072.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:04:45: 11000000 INFO @ Wed, 28 Jun 2017 02:04:49: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:49: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:49: #1 total tags in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:49: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:49: #1 tags after filtering in treatment: 11530343 INFO @ Wed, 28 Jun 2017 02:04:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:49: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:49: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:50: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:50: Process for pairing-model is terminated! cat: SRX2619072.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619072.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619072.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。