Job ID = 9385321 sra ファイルのダウンロード中... Completed: 713719K bytes transferred in 12 seconds (450046K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22040507 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2174495/SRR4254723.sra Written 22040507 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:10 22040507 reads; of these: 22040507 (100.00%) were unpaired; of these: 1857981 (8.43%) aligned 0 times 18130589 (82.26%) aligned exactly 1 time 2051937 (9.31%) aligned >1 times 91.57% overall alignment rate Time searching: 00:04:10 Overall time: 00:04:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9643747 / 20182526 = 0.4778 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 06 Aug 2017 00:54:11: # Command line: callpeak -t SRX2174495.bam -f BAM -g 12100000 -n SRX2174495.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2174495.05 # format = BAM # ChIP-seq file = ['SRX2174495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 06 Aug 2017 00:54:11: # Command line: callpeak -t SRX2174495.bam -f BAM -g 12100000 -n SRX2174495.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2174495.20 # format = BAM # ChIP-seq file = ['SRX2174495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 06 Aug 2017 00:54:11: # Command line: callpeak -t SRX2174495.bam -f BAM -g 12100000 -n SRX2174495.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2174495.10 # format = BAM # ChIP-seq file = ['SRX2174495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 06 Aug 2017 00:54:11: #1 read tag files... INFO @ Sun, 06 Aug 2017 00:54:11: #1 read tag files... INFO @ Sun, 06 Aug 2017 00:54:11: #1 read tag files... INFO @ Sun, 06 Aug 2017 00:54:11: #1 read treatment tags... INFO @ Sun, 06 Aug 2017 00:54:11: #1 read treatment tags... INFO @ Sun, 06 Aug 2017 00:54:11: #1 read treatment tags... INFO @ Sun, 06 Aug 2017 00:54:17: 1000000 INFO @ Sun, 06 Aug 2017 00:54:17: 1000000 INFO @ Sun, 06 Aug 2017 00:54:18: 1000000 INFO @ Sun, 06 Aug 2017 00:54:24: 2000000 INFO @ Sun, 06 Aug 2017 00:54:24: 2000000 INFO @ Sun, 06 Aug 2017 00:54:25: 2000000 INFO @ Sun, 06 Aug 2017 00:54:31: 3000000 INFO @ Sun, 06 Aug 2017 00:54:31: 3000000 INFO @ Sun, 06 Aug 2017 00:54:32: 3000000 INFO @ Sun, 06 Aug 2017 00:54:38: 4000000 INFO @ Sun, 06 Aug 2017 00:54:39: 4000000 INFO @ Sun, 06 Aug 2017 00:54:40: 4000000 INFO @ Sun, 06 Aug 2017 00:54:46: 5000000 INFO @ Sun, 06 Aug 2017 00:54:46: 5000000 INFO @ Sun, 06 Aug 2017 00:54:47: 5000000 INFO @ Sun, 06 Aug 2017 00:54:53: 6000000 INFO @ Sun, 06 Aug 2017 00:54:53: 6000000 INFO @ Sun, 06 Aug 2017 00:54:55: 6000000 INFO @ Sun, 06 Aug 2017 00:55:00: 7000000 INFO @ Sun, 06 Aug 2017 00:55:00: 7000000 INFO @ Sun, 06 Aug 2017 00:55:03: 7000000 INFO @ Sun, 06 Aug 2017 00:55:07: 8000000 INFO @ Sun, 06 Aug 2017 00:55:08: 8000000 INFO @ Sun, 06 Aug 2017 00:55:10: 8000000 INFO @ Sun, 06 Aug 2017 00:55:15: 9000000 INFO @ Sun, 06 Aug 2017 00:55:16: 9000000 INFO @ Sun, 06 Aug 2017 00:55:18: 9000000 INFO @ Sun, 06 Aug 2017 00:55:22: 10000000 INFO @ Sun, 06 Aug 2017 00:55:23: 10000000 INFO @ Sun, 06 Aug 2017 00:55:26: 10000000 INFO @ Sun, 06 Aug 2017 00:55:26: #1 tag size is determined as 51 bps INFO @ Sun, 06 Aug 2017 00:55:26: #1 tag size = 51 INFO @ Sun, 06 Aug 2017 00:55:26: #1 total tags in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:26: #1 user defined the maximum tags... INFO @ Sun, 06 Aug 2017 00:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 06 Aug 2017 00:55:26: #1 tags after filtering in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 06 Aug 2017 00:55:26: #1 finished! INFO @ Sun, 06 Aug 2017 00:55:26: #2 Build Peak Model... INFO @ Sun, 06 Aug 2017 00:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 06 Aug 2017 00:55:27: #2 number of paired peaks: 0 WARNING @ Sun, 06 Aug 2017 00:55:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 06 Aug 2017 00:55:27: Process for pairing-model is terminated! cat: SRX2174495.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2174495.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 06 Aug 2017 00:55:27: #1 tag size is determined as 51 bps INFO @ Sun, 06 Aug 2017 00:55:27: #1 tag size = 51 INFO @ Sun, 06 Aug 2017 00:55:27: #1 total tags in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:27: #1 user defined the maximum tags... INFO @ Sun, 06 Aug 2017 00:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 06 Aug 2017 00:55:27: #1 tags after filtering in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 06 Aug 2017 00:55:27: #1 finished! INFO @ Sun, 06 Aug 2017 00:55:27: #2 Build Peak Model... INFO @ Sun, 06 Aug 2017 00:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 06 Aug 2017 00:55:28: #2 number of paired peaks: 0 WARNING @ Sun, 06 Aug 2017 00:55:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 06 Aug 2017 00:55:28: Process for pairing-model is terminated! cat: SRX2174495.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2174495.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 06 Aug 2017 00:55:29: #1 tag size is determined as 51 bps INFO @ Sun, 06 Aug 2017 00:55:29: #1 tag size = 51 INFO @ Sun, 06 Aug 2017 00:55:29: #1 total tags in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:29: #1 user defined the maximum tags... INFO @ Sun, 06 Aug 2017 00:55:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 06 Aug 2017 00:55:29: #1 tags after filtering in treatment: 10538779 INFO @ Sun, 06 Aug 2017 00:55:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 06 Aug 2017 00:55:29: #1 finished! INFO @ Sun, 06 Aug 2017 00:55:29: #2 Build Peak Model... INFO @ Sun, 06 Aug 2017 00:55:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 06 Aug 2017 00:55:30: #2 number of paired peaks: 0 WARNING @ Sun, 06 Aug 2017 00:55:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 06 Aug 2017 00:55:30: Process for pairing-model is terminated! cat: SRX2174495.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2174495.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2174495.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。