Job ID = 2009836 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 46,782,963 reads read : 46,782,963 reads written : 46,782,963 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR546145.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:04 46782963 reads; of these: 46782963 (100.00%) were unpaired; of these: 2468825 (5.28%) aligned 0 times 30546834 (65.29%) aligned exactly 1 time 13767304 (29.43%) aligned >1 times 94.72% overall alignment rate Time searching: 00:31:04 Overall time: 00:31:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 33828194 / 44314138 = 0.7634 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:58:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:58:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:58:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:58:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:58:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:58:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:58:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:58:58: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:58:58: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:59:07: 1000000 INFO @ Fri, 05 Jul 2019 20:59:08: 1000000 INFO @ Fri, 05 Jul 2019 20:59:10: 1000000 INFO @ Fri, 05 Jul 2019 20:59:16: 2000000 INFO @ Fri, 05 Jul 2019 20:59:17: 2000000 INFO @ Fri, 05 Jul 2019 20:59:21: 2000000 INFO @ Fri, 05 Jul 2019 20:59:27: 3000000 INFO @ Fri, 05 Jul 2019 20:59:29: 3000000 INFO @ Fri, 05 Jul 2019 20:59:34: 3000000 INFO @ Fri, 05 Jul 2019 20:59:36: 4000000 INFO @ Fri, 05 Jul 2019 20:59:42: 4000000 INFO @ Fri, 05 Jul 2019 20:59:45: 5000000 INFO @ Fri, 05 Jul 2019 20:59:47: 4000000 INFO @ Fri, 05 Jul 2019 20:59:54: 6000000 INFO @ Fri, 05 Jul 2019 20:59:54: 5000000 INFO @ Fri, 05 Jul 2019 20:59:59: 5000000 INFO @ Fri, 05 Jul 2019 21:00:03: 7000000 INFO @ Fri, 05 Jul 2019 21:00:07: 6000000 INFO @ Fri, 05 Jul 2019 21:00:12: 6000000 INFO @ Fri, 05 Jul 2019 21:00:13: 8000000 INFO @ Fri, 05 Jul 2019 21:00:19: 7000000 INFO @ Fri, 05 Jul 2019 21:00:22: 9000000 INFO @ Fri, 05 Jul 2019 21:00:24: 7000000 INFO @ Fri, 05 Jul 2019 21:00:31: 10000000 INFO @ Fri, 05 Jul 2019 21:00:32: 8000000 INFO @ Fri, 05 Jul 2019 21:00:36: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 21:00:36: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 21:00:36: #1 total tags in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:00:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:00:36: #1 tags after filtering in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:00:36: #1 finished! INFO @ Fri, 05 Jul 2019 21:00:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:00:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:00:37: 8000000 INFO @ Fri, 05 Jul 2019 21:00:37: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:00:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:00:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:00:45: 9000000 INFO @ Fri, 05 Jul 2019 21:00:49: 9000000 INFO @ Fri, 05 Jul 2019 21:00:57: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 21:01:02: 10000000 INFO @ Fri, 05 Jul 2019 21:01:03: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 21:01:03: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 21:01:03: #1 total tags in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:01:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:01:04: #1 tags after filtering in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:01:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:01:04: #1 finished! INFO @ Fri, 05 Jul 2019 21:01:04: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:01:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:01:04: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:01:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:01:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:01:07: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 21:01:07: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 21:01:07: #1 total tags in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:01:07: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:01:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:01:07: #1 tags after filtering in treatment: 10485944 INFO @ Fri, 05 Jul 2019 21:01:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:01:07: #1 finished! INFO @ Fri, 05 Jul 2019 21:01:07: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:01:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:01:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:01:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180133/SRX180133.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。