Job ID = 9162245 sra ファイルのダウンロード中... Completed: 704696K bytes transferred in 9 seconds (607548K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20841756 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1791109/SRR3567984.sra Written 20841756 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:04 20841756 reads; of these: 20841756 (100.00%) were unpaired; of these: 623079 (2.99%) aligned 0 times 17600401 (84.45%) aligned exactly 1 time 2618276 (12.56%) aligned >1 times 97.01% overall alignment rate Time searching: 00:04:04 Overall time: 00:04:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7496255 / 20218677 = 0.3708 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:10:32: # Command line: callpeak -t SRX1791109.bam -f BAM -g 12100000 -n SRX1791109.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1791109.10 # format = BAM # ChIP-seq file = ['SRX1791109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:10:32: # Command line: callpeak -t SRX1791109.bam -f BAM -g 12100000 -n SRX1791109.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1791109.05 # format = BAM # ChIP-seq file = ['SRX1791109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:10:32: # Command line: callpeak -t SRX1791109.bam -f BAM -g 12100000 -n SRX1791109.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1791109.20 # format = BAM # ChIP-seq file = ['SRX1791109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:10:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:10:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:10:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:10:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:10:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:10:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:10:39: 1000000 INFO @ Wed, 28 Jun 2017 07:10:39: 1000000 INFO @ Wed, 28 Jun 2017 07:10:39: 1000000 INFO @ Wed, 28 Jun 2017 07:10:45: 2000000 INFO @ Wed, 28 Jun 2017 07:10:46: 2000000 INFO @ Wed, 28 Jun 2017 07:10:46: 2000000 INFO @ Wed, 28 Jun 2017 07:10:52: 3000000 INFO @ Wed, 28 Jun 2017 07:10:53: 3000000 INFO @ Wed, 28 Jun 2017 07:10:53: 3000000 INFO @ Wed, 28 Jun 2017 07:10:59: 4000000 INFO @ Wed, 28 Jun 2017 07:11:00: 4000000 INFO @ Wed, 28 Jun 2017 07:11:00: 4000000 INFO @ Wed, 28 Jun 2017 07:11:05: 5000000 INFO @ Wed, 28 Jun 2017 07:11:07: 5000000 INFO @ Wed, 28 Jun 2017 07:11:07: 5000000 INFO @ Wed, 28 Jun 2017 07:11:12: 6000000 INFO @ Wed, 28 Jun 2017 07:11:14: 6000000 INFO @ Wed, 28 Jun 2017 07:11:14: 6000000 INFO @ Wed, 28 Jun 2017 07:11:18: 7000000 INFO @ Wed, 28 Jun 2017 07:11:21: 7000000 INFO @ Wed, 28 Jun 2017 07:11:21: 7000000 INFO @ Wed, 28 Jun 2017 07:11:25: 8000000 INFO @ Wed, 28 Jun 2017 07:11:28: 8000000 INFO @ Wed, 28 Jun 2017 07:11:28: 8000000 INFO @ Wed, 28 Jun 2017 07:11:33: 9000000 INFO @ Wed, 28 Jun 2017 07:11:35: 9000000 INFO @ Wed, 28 Jun 2017 07:11:35: 9000000 INFO @ Wed, 28 Jun 2017 07:11:42: 10000000 INFO @ Wed, 28 Jun 2017 07:11:42: 10000000 INFO @ Wed, 28 Jun 2017 07:11:42: 10000000 INFO @ Wed, 28 Jun 2017 07:11:50: 11000000 INFO @ Wed, 28 Jun 2017 07:11:50: 11000000 INFO @ Wed, 28 Jun 2017 07:11:50: 11000000 INFO @ Wed, 28 Jun 2017 07:11:57: 12000000 INFO @ Wed, 28 Jun 2017 07:11:57: 12000000 INFO @ Wed, 28 Jun 2017 07:11:59: 12000000 INFO @ Wed, 28 Jun 2017 07:12:03: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:12:03: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:12:03: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:12:03: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:12:03: #1 total tags in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:03: #1 total tags in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:12:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:12:04: #1 tags after filtering in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:04: #1 tags after filtering in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:12:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:12:04: #1 finished! INFO @ Wed, 28 Jun 2017 07:12:04: #1 finished! INFO @ Wed, 28 Jun 2017 07:12:04: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:12:04: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:12:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:12:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:12:04: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:12:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:12:04: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 07:12:04: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:12:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:12:04: Process for pairing-model is terminated! cat: SRX1791109.10_peaks.narrowPeakcat: SRX1791109.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791109.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791109.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:12:06: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:12:06: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:12:06: #1 total tags in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:06: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:12:06: #1 tags after filtering in treatment: 12722422 INFO @ Wed, 28 Jun 2017 07:12:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:12:06: #1 finished! INFO @ Wed, 28 Jun 2017 07:12:06: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:12:07: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:12:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:12:07: Process for pairing-model is terminated! cat: SRX1791109.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791109.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791109.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。