Job ID = 2009805 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T10:59:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T10:59:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 33,120,921 reads read : 33,120,921 reads written : 33,120,921 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:31 33120921 reads; of these: 33120921 (100.00%) were unpaired; of these: 1440642 (4.35%) aligned 0 times 24803731 (74.89%) aligned exactly 1 time 6876548 (20.76%) aligned >1 times 95.65% overall alignment rate Time searching: 00:04:31 Overall time: 00:04:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 18031159 / 31680279 = 0.5692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:20:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:20:11: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:20:11: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:20:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:20:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:20:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:20:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:20:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:20:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:20:19: 1000000 INFO @ Fri, 05 Jul 2019 20:20:20: 1000000 INFO @ Fri, 05 Jul 2019 20:20:20: 1000000 INFO @ Fri, 05 Jul 2019 20:20:25: 2000000 INFO @ Fri, 05 Jul 2019 20:20:27: 2000000 INFO @ Fri, 05 Jul 2019 20:20:27: 2000000 INFO @ Fri, 05 Jul 2019 20:20:32: 3000000 INFO @ Fri, 05 Jul 2019 20:20:34: 3000000 INFO @ Fri, 05 Jul 2019 20:20:34: 3000000 INFO @ Fri, 05 Jul 2019 20:20:39: 4000000 INFO @ Fri, 05 Jul 2019 20:20:41: 4000000 INFO @ Fri, 05 Jul 2019 20:20:41: 4000000 INFO @ Fri, 05 Jul 2019 20:20:46: 5000000 INFO @ Fri, 05 Jul 2019 20:20:47: 5000000 INFO @ Fri, 05 Jul 2019 20:20:48: 5000000 INFO @ Fri, 05 Jul 2019 20:20:52: 6000000 INFO @ Fri, 05 Jul 2019 20:20:54: 6000000 INFO @ Fri, 05 Jul 2019 20:20:55: 6000000 INFO @ Fri, 05 Jul 2019 20:20:59: 7000000 INFO @ Fri, 05 Jul 2019 20:21:01: 7000000 INFO @ Fri, 05 Jul 2019 20:21:01: 7000000 INFO @ Fri, 05 Jul 2019 20:21:06: 8000000 INFO @ Fri, 05 Jul 2019 20:21:08: 8000000 INFO @ Fri, 05 Jul 2019 20:21:09: 8000000 INFO @ Fri, 05 Jul 2019 20:21:13: 9000000 INFO @ Fri, 05 Jul 2019 20:21:15: 9000000 INFO @ Fri, 05 Jul 2019 20:21:15: 9000000 INFO @ Fri, 05 Jul 2019 20:21:21: 10000000 INFO @ Fri, 05 Jul 2019 20:21:21: 10000000 INFO @ Fri, 05 Jul 2019 20:21:22: 10000000 INFO @ Fri, 05 Jul 2019 20:21:28: 11000000 INFO @ Fri, 05 Jul 2019 20:21:28: 11000000 INFO @ Fri, 05 Jul 2019 20:21:29: 11000000 INFO @ Fri, 05 Jul 2019 20:21:35: 12000000 INFO @ Fri, 05 Jul 2019 20:21:35: 12000000 INFO @ Fri, 05 Jul 2019 20:21:39: 12000000 INFO @ Fri, 05 Jul 2019 20:21:42: 13000000 INFO @ Fri, 05 Jul 2019 20:21:43: 13000000 INFO @ Fri, 05 Jul 2019 20:21:46: 13000000 INFO @ Fri, 05 Jul 2019 20:21:46: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:21:46: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:21:46: #1 total tags in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:21:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:21:47: #1 tags after filtering in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:21:47: #1 finished! INFO @ Fri, 05 Jul 2019 20:21:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:21:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:21:48: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:21:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:21:48: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 20:21:48: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:21:48: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:21:48: #1 total tags in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:21:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:21:48: #1 tags after filtering in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:21:48: #1 finished! INFO @ Fri, 05 Jul 2019 20:21:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:21:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:21:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:21:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:21:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:21:51: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:21:51: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:21:51: #1 total tags in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:51: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:21:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:21:51: #1 tags after filtering in treatment: 13649120 INFO @ Fri, 05 Jul 2019 20:21:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:21:51: #1 finished! INFO @ Fri, 05 Jul 2019 20:21:51: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:21:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:21:52: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:21:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:21:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170679/SRX170679.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。