Job ID = 14520416 SRX = SRX11781138 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:03:10 prefetch.2.10.7: 1) Downloading 'SRR15481081'... 2022-01-15T10:03:11 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:03:27 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:03:28 prefetch.2.10.7: 'SRR15481081' is valid 2022-01-15T10:03:28 prefetch.2.10.7: 1) 'SRR15481081' was downloaded successfully 2022-01-15T10:03:28 prefetch.2.10.7: 'SRR15481081' has 0 unresolved dependencies Read 7811807 spots for SRR15481081/SRR15481081.sra Written 7811807 spots for SRR15481081/SRR15481081.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:21 7811807 reads; of these: 7811807 (100.00%) were paired; of these: 4587992 (58.73%) aligned concordantly 0 times 2691826 (34.46%) aligned concordantly exactly 1 time 531989 (6.81%) aligned concordantly >1 times ---- 4587992 pairs aligned concordantly 0 times; of these: 23009 (0.50%) aligned discordantly 1 time ---- 4564983 pairs aligned 0 times concordantly or discordantly; of these: 9129966 mates make up the pairs; of these: 4922914 (53.92%) aligned 0 times 3493777 (38.27%) aligned exactly 1 time 713275 (7.81%) aligned >1 times 68.49% overall alignment rate Time searching: 00:08:21 Overall time: 00:08:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 173002 / 3246142 = 0.0533 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:09: 1000000 INFO @ Sat, 15 Jan 2022 19:18:18: 2000000 INFO @ Sat, 15 Jan 2022 19:18:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:33: 4000000 INFO @ Sat, 15 Jan 2022 19:18:41: 1000000 INFO @ Sat, 15 Jan 2022 19:18:43: 5000000 INFO @ Sat, 15 Jan 2022 19:18:49: 2000000 INFO @ Sat, 15 Jan 2022 19:18:53: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:58: 3000000 INFO @ Sat, 15 Jan 2022 19:19:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:03: 7000000 INFO @ Sat, 15 Jan 2022 19:19:08: 4000000 INFO @ Sat, 15 Jan 2022 19:19:12: 8000000 INFO @ Sat, 15 Jan 2022 19:19:12: 1000000 INFO @ Sat, 15 Jan 2022 19:19:20: 5000000 INFO @ Sat, 15 Jan 2022 19:19:24: 9000000 INFO @ Sat, 15 Jan 2022 19:19:24: 2000000 INFO @ Sat, 15 Jan 2022 19:19:33: 6000000 INFO @ Sat, 15 Jan 2022 19:19:35: 10000000 INFO @ Sat, 15 Jan 2022 19:19:37: 3000000 INFO @ Sat, 15 Jan 2022 19:19:39: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:19:39: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:19:39: #1 total tags in treatment: 3051466 INFO @ Sat, 15 Jan 2022 19:19:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:39: #1 tags after filtering in treatment: 2460082 INFO @ Sat, 15 Jan 2022 19:19:39: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:19:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:40: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:19:40: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:43: 7000000 INFO @ Sat, 15 Jan 2022 19:19:47: 4000000 INFO @ Sat, 15 Jan 2022 19:19:54: 8000000 INFO @ Sat, 15 Jan 2022 19:19:57: 5000000 INFO @ Sat, 15 Jan 2022 19:20:04: 9000000 INFO @ Sat, 15 Jan 2022 19:20:06: 6000000 INFO @ Sat, 15 Jan 2022 19:20:14: 10000000 INFO @ Sat, 15 Jan 2022 19:20:16: 7000000 INFO @ Sat, 15 Jan 2022 19:20:17: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:20:17: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:20:17: #1 total tags in treatment: 3051466 INFO @ Sat, 15 Jan 2022 19:20:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:18: #1 tags after filtering in treatment: 2460082 INFO @ Sat, 15 Jan 2022 19:20:18: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:20:18: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:18: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:20:18: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:20:27: 8000000 INFO @ Sat, 15 Jan 2022 19:20:40: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:20:54: 10000000 INFO @ Sat, 15 Jan 2022 19:20:59: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:20:59: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:20:59: #1 total tags in treatment: 3051466 INFO @ Sat, 15 Jan 2022 19:20:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:59: #1 tags after filtering in treatment: 2460082 INFO @ Sat, 15 Jan 2022 19:20:59: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:20:59: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:59: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:20:59: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781138/SRX11781138.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling