Job ID = 14520615 SRX = SRX11781081 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:17 prefetch.2.10.7: 1) Downloading 'SRR15481024'... 2022-01-15T10:27:17 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:28:52 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:28:52 prefetch.2.10.7: 1) 'SRR15481024' was downloaded successfully 2022-01-15T10:28:52 prefetch.2.10.7: 'SRR15481024' has 0 unresolved dependencies Read 16005640 spots for SRR15481024/SRR15481024.sra Written 16005640 spots for SRR15481024/SRR15481024.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:18 16005640 reads; of these: 16005640 (100.00%) were paired; of these: 3240509 (20.25%) aligned concordantly 0 times 11332985 (70.81%) aligned concordantly exactly 1 time 1432146 (8.95%) aligned concordantly >1 times ---- 3240509 pairs aligned concordantly 0 times; of these: 173019 (5.34%) aligned discordantly 1 time ---- 3067490 pairs aligned 0 times concordantly or discordantly; of these: 6134980 mates make up the pairs; of these: 3467181 (56.51%) aligned 0 times 2282247 (37.20%) aligned exactly 1 time 385552 (6.28%) aligned >1 times 89.17% overall alignment rate Time searching: 00:14:18 Overall time: 00:14:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3272334 / 12936434 = 0.2530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:26: 1000000 INFO @ Sat, 15 Jan 2022 20:05:33: 2000000 INFO @ Sat, 15 Jan 2022 20:05:39: 3000000 INFO @ Sat, 15 Jan 2022 20:05:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:53: 5000000 INFO @ Sat, 15 Jan 2022 20:05:56: 1000000 INFO @ Sat, 15 Jan 2022 20:06:00: 6000000 INFO @ Sat, 15 Jan 2022 20:06:02: 2000000 INFO @ Sat, 15 Jan 2022 20:06:07: 7000000 INFO @ Sat, 15 Jan 2022 20:06:08: 3000000 INFO @ Sat, 15 Jan 2022 20:06:14: 8000000 INFO @ Sat, 15 Jan 2022 20:06:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:21: 9000000 INFO @ Sat, 15 Jan 2022 20:06:22: 5000000 INFO @ Sat, 15 Jan 2022 20:06:26: 1000000 INFO @ Sat, 15 Jan 2022 20:06:28: 10000000 INFO @ Sat, 15 Jan 2022 20:06:28: 6000000 INFO @ Sat, 15 Jan 2022 20:06:32: 2000000 INFO @ Sat, 15 Jan 2022 20:06:35: 7000000 INFO @ Sat, 15 Jan 2022 20:06:35: 11000000 INFO @ Sat, 15 Jan 2022 20:06:38: 3000000 INFO @ Sat, 15 Jan 2022 20:06:41: 8000000 INFO @ Sat, 15 Jan 2022 20:06:42: 12000000 INFO @ Sat, 15 Jan 2022 20:06:45: 4000000 INFO @ Sat, 15 Jan 2022 20:06:48: 9000000 INFO @ Sat, 15 Jan 2022 20:06:48: 13000000 INFO @ Sat, 15 Jan 2022 20:06:51: 5000000 INFO @ Sat, 15 Jan 2022 20:06:54: 10000000 INFO @ Sat, 15 Jan 2022 20:06:55: 14000000 INFO @ Sat, 15 Jan 2022 20:06:58: 6000000 INFO @ Sat, 15 Jan 2022 20:07:00: 11000000 INFO @ Sat, 15 Jan 2022 20:07:03: 15000000 INFO @ Sat, 15 Jan 2022 20:07:04: 7000000 INFO @ Sat, 15 Jan 2022 20:07:07: 12000000 INFO @ Sat, 15 Jan 2022 20:07:09: 16000000 INFO @ Sat, 15 Jan 2022 20:07:10: 8000000 INFO @ Sat, 15 Jan 2022 20:07:13: 13000000 INFO @ Sat, 15 Jan 2022 20:07:16: 17000000 INFO @ Sat, 15 Jan 2022 20:07:16: 9000000 INFO @ Sat, 15 Jan 2022 20:07:20: 14000000 INFO @ Sat, 15 Jan 2022 20:07:23: 10000000 INFO @ Sat, 15 Jan 2022 20:07:23: 18000000 INFO @ Sat, 15 Jan 2022 20:07:27: 15000000 INFO @ Sat, 15 Jan 2022 20:07:29: 11000000 INFO @ Sat, 15 Jan 2022 20:07:30: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:07:33: 16000000 INFO @ Sat, 15 Jan 2022 20:07:35: 12000000 INFO @ Sat, 15 Jan 2022 20:07:37: 20000000 INFO @ Sat, 15 Jan 2022 20:07:40: 17000000 INFO @ Sat, 15 Jan 2022 20:07:42: 13000000 INFO @ Sat, 15 Jan 2022 20:07:44: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:07:46: 18000000 INFO @ Sat, 15 Jan 2022 20:07:48: 14000000 INFO @ Sat, 15 Jan 2022 20:07:51: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:07:51: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:07:51: #1 total tags in treatment: 9514682 INFO @ Sat, 15 Jan 2022 20:07:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:51: #1 tags after filtering in treatment: 5619605 INFO @ Sat, 15 Jan 2022 20:07:51: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:07:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:07:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:54: 19000000 INFO @ Sat, 15 Jan 2022 20:07:54: 15000000 INFO @ Sat, 15 Jan 2022 20:08:00: 20000000 INFO @ Sat, 15 Jan 2022 20:08:00: 16000000 INFO @ Sat, 15 Jan 2022 20:08:06: 17000000 INFO @ Sat, 15 Jan 2022 20:08:07: 21000000 INFO @ Sat, 15 Jan 2022 20:08:13: 18000000 INFO @ Sat, 15 Jan 2022 20:08:13: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:13: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:13: #1 total tags in treatment: 9514682 INFO @ Sat, 15 Jan 2022 20:08:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:13: #1 tags after filtering in treatment: 5619605 INFO @ Sat, 15 Jan 2022 20:08:13: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:08:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:08:19: 19000000 INFO @ Sat, 15 Jan 2022 20:08:24: 20000000 INFO @ Sat, 15 Jan 2022 20:08:30: 21000000 INFO @ Sat, 15 Jan 2022 20:08:36: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:36: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:36: #1 total tags in treatment: 9514682 INFO @ Sat, 15 Jan 2022 20:08:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:36: #1 tags after filtering in treatment: 5619605 INFO @ Sat, 15 Jan 2022 20:08:36: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:08:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781081/SRX11781081.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling