Job ID = 14520593 SRX = SRX11781071 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:25:04 prefetch.2.10.7: 1) Downloading 'SRR15481014'... 2022-01-15T10:25:04 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:25:50 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:25:50 prefetch.2.10.7: 1) 'SRR15481014' was downloaded successfully 2022-01-15T10:25:50 prefetch.2.10.7: 'SRR15481014' has 0 unresolved dependencies Read 12495536 spots for SRR15481014/SRR15481014.sra Written 12495536 spots for SRR15481014/SRR15481014.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:35 12495536 reads; of these: 12495536 (100.00%) were paired; of these: 2027124 (16.22%) aligned concordantly 0 times 9114652 (72.94%) aligned concordantly exactly 1 time 1353760 (10.83%) aligned concordantly >1 times ---- 2027124 pairs aligned concordantly 0 times; of these: 89406 (4.41%) aligned discordantly 1 time ---- 1937718 pairs aligned 0 times concordantly or discordantly; of these: 3875436 mates make up the pairs; of these: 2435119 (62.83%) aligned 0 times 1224563 (31.60%) aligned exactly 1 time 215754 (5.57%) aligned >1 times 90.26% overall alignment rate Time searching: 00:09:35 Overall time: 00:09:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1100040 / 10556990 = 0.1042 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:44:43: 1000000 INFO @ Sat, 15 Jan 2022 19:44:51: 2000000 INFO @ Sat, 15 Jan 2022 19:44:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:06: 4000000 INFO @ Sat, 15 Jan 2022 19:45:13: 1000000 INFO @ Sat, 15 Jan 2022 19:45:15: 5000000 INFO @ Sat, 15 Jan 2022 19:45:21: 2000000 INFO @ Sat, 15 Jan 2022 19:45:24: 6000000 INFO @ Sat, 15 Jan 2022 19:45:29: 3000000 INFO @ Sat, 15 Jan 2022 19:45:32: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:37: 4000000 INFO @ Sat, 15 Jan 2022 19:45:40: 8000000 INFO @ Sat, 15 Jan 2022 19:45:43: 1000000 INFO @ Sat, 15 Jan 2022 19:45:45: 5000000 INFO @ Sat, 15 Jan 2022 19:45:49: 9000000 INFO @ Sat, 15 Jan 2022 19:45:51: 2000000 INFO @ Sat, 15 Jan 2022 19:45:54: 6000000 INFO @ Sat, 15 Jan 2022 19:45:58: 10000000 INFO @ Sat, 15 Jan 2022 19:45:59: 3000000 INFO @ Sat, 15 Jan 2022 19:46:02: 7000000 INFO @ Sat, 15 Jan 2022 19:46:06: 11000000 INFO @ Sat, 15 Jan 2022 19:46:07: 4000000 INFO @ Sat, 15 Jan 2022 19:46:10: 8000000 INFO @ Sat, 15 Jan 2022 19:46:15: 12000000 INFO @ Sat, 15 Jan 2022 19:46:15: 5000000 INFO @ Sat, 15 Jan 2022 19:46:18: 9000000 INFO @ Sat, 15 Jan 2022 19:46:23: 6000000 INFO @ Sat, 15 Jan 2022 19:46:24: 13000000 INFO @ Sat, 15 Jan 2022 19:46:26: 10000000 INFO @ Sat, 15 Jan 2022 19:46:31: 7000000 INFO @ Sat, 15 Jan 2022 19:46:33: 14000000 INFO @ Sat, 15 Jan 2022 19:46:34: 11000000 INFO @ Sat, 15 Jan 2022 19:46:39: 8000000 INFO @ Sat, 15 Jan 2022 19:46:41: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:46:42: 12000000 INFO @ Sat, 15 Jan 2022 19:46:47: 9000000 INFO @ Sat, 15 Jan 2022 19:46:50: 16000000 INFO @ Sat, 15 Jan 2022 19:46:51: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:46:55: 10000000 INFO @ Sat, 15 Jan 2022 19:46:58: 17000000 INFO @ Sat, 15 Jan 2022 19:46:59: 14000000 INFO @ Sat, 15 Jan 2022 19:47:03: 11000000 INFO @ Sat, 15 Jan 2022 19:47:07: 15000000 INFO @ Sat, 15 Jan 2022 19:47:07: 18000000 INFO @ Sat, 15 Jan 2022 19:47:11: 12000000 INFO @ Sat, 15 Jan 2022 19:47:15: 16000000 INFO @ Sat, 15 Jan 2022 19:47:16: 19000000 INFO @ Sat, 15 Jan 2022 19:47:20: 13000000 INFO @ Sat, 15 Jan 2022 19:47:23: 17000000 INFO @ Sat, 15 Jan 2022 19:47:24: 20000000 INFO @ Sat, 15 Jan 2022 19:47:27: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:47:27: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:47:27: #1 total tags in treatment: 9372081 INFO @ Sat, 15 Jan 2022 19:47:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:27: #1 tags after filtering in treatment: 5957924 INFO @ Sat, 15 Jan 2022 19:47:27: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:47:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:28: 14000000 INFO @ Sat, 15 Jan 2022 19:47:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:31: 18000000 INFO @ Sat, 15 Jan 2022 19:47:35: 15000000 INFO @ Sat, 15 Jan 2022 19:47:39: 19000000 INFO @ Sat, 15 Jan 2022 19:47:43: 16000000 INFO @ Sat, 15 Jan 2022 19:47:46: 20000000 INFO @ Sat, 15 Jan 2022 19:47:49: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:47:49: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:47:49: #1 total tags in treatment: 9372081 INFO @ Sat, 15 Jan 2022 19:47:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:49: #1 tags after filtering in treatment: 5957924 INFO @ Sat, 15 Jan 2022 19:47:49: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:47:49: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:51: 17000000 INFO @ Sat, 15 Jan 2022 19:47:58: 18000000 INFO @ Sat, 15 Jan 2022 19:48:05: 19000000 INFO @ Sat, 15 Jan 2022 19:48:12: 20000000 INFO @ Sat, 15 Jan 2022 19:48:15: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:48:15: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:48:15: #1 total tags in treatment: 9372081 INFO @ Sat, 15 Jan 2022 19:48:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:15: #1 tags after filtering in treatment: 5957924 INFO @ Sat, 15 Jan 2022 19:48:15: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:48:15: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781071/SRX11781071.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling