Job ID = 14521777 SRX = SRX11320607 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36341034 spots for SRR15008443/SRR15008443.sra Written 36341034 spots for SRR15008443/SRR15008443.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 36341034 reads; of these: 36341034 (100.00%) were unpaired; of these: 6442228 (17.73%) aligned 0 times 26723949 (73.54%) aligned exactly 1 time 3174857 (8.74%) aligned >1 times 82.27% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18011214 / 29898806 = 0.6024 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:08: 1000000 INFO @ Sat, 15 Jan 2022 21:47:14: 2000000 INFO @ Sat, 15 Jan 2022 21:47:20: 3000000 INFO @ Sat, 15 Jan 2022 21:47:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:32: 5000000 INFO @ Sat, 15 Jan 2022 21:47:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:38: 6000000 INFO @ Sat, 15 Jan 2022 21:47:38: 1000000 INFO @ Sat, 15 Jan 2022 21:47:45: 7000000 INFO @ Sat, 15 Jan 2022 21:47:45: 2000000 INFO @ Sat, 15 Jan 2022 21:47:51: 8000000 INFO @ Sat, 15 Jan 2022 21:47:52: 3000000 INFO @ Sat, 15 Jan 2022 21:47:57: 9000000 INFO @ Sat, 15 Jan 2022 21:47:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:04: 10000000 INFO @ Sat, 15 Jan 2022 21:48:05: 5000000 INFO @ Sat, 15 Jan 2022 21:48:08: 1000000 INFO @ Sat, 15 Jan 2022 21:48:10: 11000000 INFO @ Sat, 15 Jan 2022 21:48:11: 6000000 INFO @ Sat, 15 Jan 2022 21:48:13: 2000000 INFO @ Sat, 15 Jan 2022 21:48:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:48:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:48:16: #1 total tags in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:48:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:16: #1 tags after filtering in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:48:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:48:16: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:17: 7000000 INFO @ Sat, 15 Jan 2022 21:48:19: 3000000 INFO @ Sat, 15 Jan 2022 21:48:23: 8000000 INFO @ Sat, 15 Jan 2022 21:48:25: 4000000 INFO @ Sat, 15 Jan 2022 21:48:29: 9000000 INFO @ Sat, 15 Jan 2022 21:48:31: 5000000 INFO @ Sat, 15 Jan 2022 21:48:35: 10000000 INFO @ Sat, 15 Jan 2022 21:48:36: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:48:41: 11000000 INFO @ Sat, 15 Jan 2022 21:48:42: 7000000 INFO @ Sat, 15 Jan 2022 21:48:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:48:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:48:47: #1 total tags in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:48:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:47: #1 tags after filtering in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:48:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:48:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:47: 8000000 INFO @ Sat, 15 Jan 2022 21:48:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:48:53: 9000000 INFO @ Sat, 15 Jan 2022 21:48:58: 10000000 INFO @ Sat, 15 Jan 2022 21:49:03: 11000000 INFO @ Sat, 15 Jan 2022 21:49:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:49:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:49:08: #1 total tags in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:49:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:08: #1 tags after filtering in treatment: 11887592 INFO @ Sat, 15 Jan 2022 21:49:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:49:08: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320607/SRX11320607.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling