Job ID = 14521771 SRX = SRX11320601 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32835506 spots for SRR15008431/SRR15008431.sra Written 32835506 spots for SRR15008431/SRR15008431.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:13 32835506 reads; of these: 32835506 (100.00%) were unpaired; of these: 8060911 (24.55%) aligned 0 times 22241822 (67.74%) aligned exactly 1 time 2532773 (7.71%) aligned >1 times 75.45% overall alignment rate Time searching: 00:04:13 Overall time: 00:04:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14272695 / 24774595 = 0.5761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:06: 1000000 INFO @ Sat, 15 Jan 2022 21:44:11: 2000000 INFO @ Sat, 15 Jan 2022 21:44:16: 3000000 INFO @ Sat, 15 Jan 2022 21:44:21: 4000000 INFO @ Sat, 15 Jan 2022 21:44:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:30: 6000000 INFO @ Sat, 15 Jan 2022 21:44:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:35: 7000000 INFO @ Sat, 15 Jan 2022 21:44:38: 1000000 INFO @ Sat, 15 Jan 2022 21:44:41: 8000000 INFO @ Sat, 15 Jan 2022 21:44:44: 2000000 INFO @ Sat, 15 Jan 2022 21:44:46: 9000000 INFO @ Sat, 15 Jan 2022 21:44:51: 3000000 INFO @ Sat, 15 Jan 2022 21:44:51: 10000000 INFO @ Sat, 15 Jan 2022 21:44:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:44:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:44:54: #1 total tags in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:44:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:44:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:44:54: #1 tags after filtering in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:44:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:44:54: #1 finished! INFO @ Sat, 15 Jan 2022 21:44:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:44:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:44:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:44:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:44:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:44:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:45:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:03: 5000000 INFO @ Sat, 15 Jan 2022 21:45:07: 1000000 INFO @ Sat, 15 Jan 2022 21:45:09: 6000000 INFO @ Sat, 15 Jan 2022 21:45:12: 2000000 INFO @ Sat, 15 Jan 2022 21:45:15: 7000000 INFO @ Sat, 15 Jan 2022 21:45:17: 3000000 INFO @ Sat, 15 Jan 2022 21:45:21: 8000000 INFO @ Sat, 15 Jan 2022 21:45:23: 4000000 INFO @ Sat, 15 Jan 2022 21:45:28: 9000000 INFO @ Sat, 15 Jan 2022 21:45:28: 5000000 INFO @ Sat, 15 Jan 2022 21:45:33: 6000000 INFO @ Sat, 15 Jan 2022 21:45:34: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:45:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:45:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:45:37: #1 total tags in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:45:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:37: #1 tags after filtering in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:45:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:37: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:45:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:45:38: 7000000 INFO @ Sat, 15 Jan 2022 21:45:43: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:45:48: 9000000 INFO @ Sat, 15 Jan 2022 21:45:53: 10000000 INFO @ Sat, 15 Jan 2022 21:45:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:45:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:45:56: #1 total tags in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:45:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:56: #1 tags after filtering in treatment: 10501900 INFO @ Sat, 15 Jan 2022 21:45:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:56: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:45:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320601/SRX11320601.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling