Job ID = 14520152 SRX = SRX10701692 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42143746 spots for SRR14347842/SRR14347842.sra Written 42143746 spots for SRR14347842/SRR14347842.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:04 42143746 reads; of these: 42143746 (100.00%) were paired; of these: 3370826 (8.00%) aligned concordantly 0 times 32785265 (77.79%) aligned concordantly exactly 1 time 5987655 (14.21%) aligned concordantly >1 times ---- 3370826 pairs aligned concordantly 0 times; of these: 765905 (22.72%) aligned discordantly 1 time ---- 2604921 pairs aligned 0 times concordantly or discordantly; of these: 5209842 mates make up the pairs; of these: 3796037 (72.86%) aligned 0 times 896350 (17.20%) aligned exactly 1 time 517455 (9.93%) aligned >1 times 95.50% overall alignment rate Time searching: 00:24:04 Overall time: 00:24:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 13432893 / 39509679 = 0.3400 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:09:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:09:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:09:35: 1000000 INFO @ Sat, 15 Jan 2022 19:09:39: 2000000 INFO @ Sat, 15 Jan 2022 19:09:44: 3000000 INFO @ Sat, 15 Jan 2022 19:09:48: 4000000 INFO @ Sat, 15 Jan 2022 19:09:53: 5000000 INFO @ Sat, 15 Jan 2022 19:09:57: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:10:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:10:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:10:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:10:01: 7000000 INFO @ Sat, 15 Jan 2022 19:10:04: 1000000 INFO @ Sat, 15 Jan 2022 19:10:05: 8000000 INFO @ Sat, 15 Jan 2022 19:10:08: 2000000 INFO @ Sat, 15 Jan 2022 19:10:09: 9000000 INFO @ Sat, 15 Jan 2022 19:10:12: 3000000 INFO @ Sat, 15 Jan 2022 19:10:14: 10000000 INFO @ Sat, 15 Jan 2022 19:10:17: 4000000 INFO @ Sat, 15 Jan 2022 19:10:18: 11000000 INFO @ Sat, 15 Jan 2022 19:10:21: 5000000 INFO @ Sat, 15 Jan 2022 19:10:23: 12000000 INFO @ Sat, 15 Jan 2022 19:10:25: 6000000 INFO @ Sat, 15 Jan 2022 19:10:28: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:10:29: 7000000 INFO @ Sat, 15 Jan 2022 19:10:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:10:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:10:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:10:32: 14000000 INFO @ Sat, 15 Jan 2022 19:10:34: 8000000 INFO @ Sat, 15 Jan 2022 19:10:35: 1000000 INFO @ Sat, 15 Jan 2022 19:10:36: 15000000 INFO @ Sat, 15 Jan 2022 19:10:38: 9000000 INFO @ Sat, 15 Jan 2022 19:10:40: 2000000 INFO @ Sat, 15 Jan 2022 19:10:41: 16000000 INFO @ Sat, 15 Jan 2022 19:10:42: 10000000 INFO @ Sat, 15 Jan 2022 19:10:45: 3000000 INFO @ Sat, 15 Jan 2022 19:10:45: 17000000 INFO @ Sat, 15 Jan 2022 19:10:46: 11000000 INFO @ Sat, 15 Jan 2022 19:10:49: 18000000 INFO @ Sat, 15 Jan 2022 19:10:50: 4000000 INFO @ Sat, 15 Jan 2022 19:10:51: 12000000 INFO @ Sat, 15 Jan 2022 19:10:53: 19000000 INFO @ Sat, 15 Jan 2022 19:10:55: 5000000 INFO @ Sat, 15 Jan 2022 19:10:55: 13000000 INFO @ Sat, 15 Jan 2022 19:10:58: 20000000 INFO @ Sat, 15 Jan 2022 19:10:59: 14000000 INFO @ Sat, 15 Jan 2022 19:11:00: 6000000 INFO @ Sat, 15 Jan 2022 19:11:02: 21000000 INFO @ Sat, 15 Jan 2022 19:11:03: 15000000 INFO @ Sat, 15 Jan 2022 19:11:05: 7000000 INFO @ Sat, 15 Jan 2022 19:11:06: 22000000 INFO @ Sat, 15 Jan 2022 19:11:07: 16000000 INFO @ Sat, 15 Jan 2022 19:11:10: 8000000 INFO @ Sat, 15 Jan 2022 19:11:10: 23000000 INFO @ Sat, 15 Jan 2022 19:11:12: 17000000 INFO @ Sat, 15 Jan 2022 19:11:15: 24000000 INFO @ Sat, 15 Jan 2022 19:11:16: 9000000 INFO @ Sat, 15 Jan 2022 19:11:16: 18000000 INFO @ Sat, 15 Jan 2022 19:11:19: 25000000 INFO @ Sat, 15 Jan 2022 19:11:21: 19000000 INFO @ Sat, 15 Jan 2022 19:11:21: 10000000 INFO @ Sat, 15 Jan 2022 19:11:23: 26000000 INFO @ Sat, 15 Jan 2022 19:11:25: 20000000 INFO @ Sat, 15 Jan 2022 19:11:26: 11000000 INFO @ Sat, 15 Jan 2022 19:11:28: 27000000 INFO @ Sat, 15 Jan 2022 19:11:29: 21000000 INFO @ Sat, 15 Jan 2022 19:11:31: 12000000 INFO @ Sat, 15 Jan 2022 19:11:32: 28000000 INFO @ Sat, 15 Jan 2022 19:11:34: 22000000 INFO @ Sat, 15 Jan 2022 19:11:36: 29000000 INFO @ Sat, 15 Jan 2022 19:11:36: 13000000 INFO @ Sat, 15 Jan 2022 19:11:38: 23000000 INFO @ Sat, 15 Jan 2022 19:11:40: 30000000 INFO @ Sat, 15 Jan 2022 19:11:41: 14000000 INFO @ Sat, 15 Jan 2022 19:11:42: 24000000 INFO @ Sat, 15 Jan 2022 19:11:45: 31000000 INFO @ Sat, 15 Jan 2022 19:11:46: 15000000 INFO @ Sat, 15 Jan 2022 19:11:46: 25000000 INFO @ Sat, 15 Jan 2022 19:11:49: 32000000 INFO @ Sat, 15 Jan 2022 19:11:51: 26000000 INFO @ Sat, 15 Jan 2022 19:11:51: 16000000 INFO @ Sat, 15 Jan 2022 19:11:53: 33000000 INFO @ Sat, 15 Jan 2022 19:11:55: 27000000 INFO @ Sat, 15 Jan 2022 19:11:56: 17000000 INFO @ Sat, 15 Jan 2022 19:11:58: 34000000 INFO @ Sat, 15 Jan 2022 19:11:59: 28000000 INFO @ Sat, 15 Jan 2022 19:12:01: 18000000 INFO @ Sat, 15 Jan 2022 19:12:02: 35000000 INFO @ Sat, 15 Jan 2022 19:12:03: 29000000 INFO @ Sat, 15 Jan 2022 19:12:06: 19000000 INFO @ Sat, 15 Jan 2022 19:12:07: 36000000 INFO @ Sat, 15 Jan 2022 19:12:08: 30000000 INFO @ Sat, 15 Jan 2022 19:12:11: 37000000 INFO @ Sat, 15 Jan 2022 19:12:11: 20000000 INFO @ Sat, 15 Jan 2022 19:12:12: 31000000 INFO @ Sat, 15 Jan 2022 19:12:15: 38000000 INFO @ Sat, 15 Jan 2022 19:12:16: 32000000 INFO @ Sat, 15 Jan 2022 19:12:16: 21000000 INFO @ Sat, 15 Jan 2022 19:12:19: 39000000 INFO @ Sat, 15 Jan 2022 19:12:20: 33000000 INFO @ Sat, 15 Jan 2022 19:12:21: 22000000 INFO @ Sat, 15 Jan 2022 19:12:23: 40000000 INFO @ Sat, 15 Jan 2022 19:12:24: 34000000 INFO @ Sat, 15 Jan 2022 19:12:26: 23000000 INFO @ Sat, 15 Jan 2022 19:12:28: 41000000 INFO @ Sat, 15 Jan 2022 19:12:29: 35000000 INFO @ Sat, 15 Jan 2022 19:12:31: 24000000 INFO @ Sat, 15 Jan 2022 19:12:32: 42000000 INFO @ Sat, 15 Jan 2022 19:12:33: 36000000 INFO @ Sat, 15 Jan 2022 19:12:36: 25000000 INFO @ Sat, 15 Jan 2022 19:12:36: 43000000 INFO @ Sat, 15 Jan 2022 19:12:37: 37000000 INFO @ Sat, 15 Jan 2022 19:12:40: 44000000 INFO @ Sat, 15 Jan 2022 19:12:41: 26000000 INFO @ Sat, 15 Jan 2022 19:12:41: 38000000 INFO @ Sat, 15 Jan 2022 19:12:45: 45000000 INFO @ Sat, 15 Jan 2022 19:12:46: 39000000 INFO @ Sat, 15 Jan 2022 19:12:46: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:12:49: 46000000 INFO @ Sat, 15 Jan 2022 19:12:50: 40000000 INFO @ Sat, 15 Jan 2022 19:12:51: 28000000 INFO @ Sat, 15 Jan 2022 19:12:53: 47000000 INFO @ Sat, 15 Jan 2022 19:12:54: 41000000 INFO @ Sat, 15 Jan 2022 19:12:56: 29000000 INFO @ Sat, 15 Jan 2022 19:12:57: 48000000 INFO @ Sat, 15 Jan 2022 19:12:58: 42000000 INFO @ Sat, 15 Jan 2022 19:13:00: 30000000 INFO @ Sat, 15 Jan 2022 19:13:02: 49000000 INFO @ Sat, 15 Jan 2022 19:13:03: 43000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:13:05: 31000000 INFO @ Sat, 15 Jan 2022 19:13:06: 50000000 INFO @ Sat, 15 Jan 2022 19:13:07: 44000000 INFO @ Sat, 15 Jan 2022 19:13:10: 51000000 INFO @ Sat, 15 Jan 2022 19:13:10: 32000000 INFO @ Sat, 15 Jan 2022 19:13:11: 45000000 INFO @ Sat, 15 Jan 2022 19:13:14: 52000000 INFO @ Sat, 15 Jan 2022 19:13:15: 33000000 INFO @ Sat, 15 Jan 2022 19:13:15: 46000000 INFO @ Sat, 15 Jan 2022 19:13:19: 53000000 INFO @ Sat, 15 Jan 2022 19:13:20: 47000000 INFO @ Sat, 15 Jan 2022 19:13:20: 34000000 INFO @ Sat, 15 Jan 2022 19:13:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:13:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:13:22: #1 total tags in treatment: 25474694 INFO @ Sat, 15 Jan 2022 19:13:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:22: #1 tags after filtering in treatment: 8577085 INFO @ Sat, 15 Jan 2022 19:13:22: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:13:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:13:24: 48000000 INFO @ Sat, 15 Jan 2022 19:13:25: 35000000 INFO @ Sat, 15 Jan 2022 19:13:28: 49000000 INFO @ Sat, 15 Jan 2022 19:13:30: 36000000 INFO @ Sat, 15 Jan 2022 19:13:32: 50000000 INFO @ Sat, 15 Jan 2022 19:13:35: 37000000 INFO @ Sat, 15 Jan 2022 19:13:36: 51000000 INFO @ Sat, 15 Jan 2022 19:13:40: 38000000 INFO @ Sat, 15 Jan 2022 19:13:41: 52000000 INFO @ Sat, 15 Jan 2022 19:13:45: 39000000 INFO @ Sat, 15 Jan 2022 19:13:45: 53000000 INFO @ Sat, 15 Jan 2022 19:13:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:13:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:13:48: #1 total tags in treatment: 25474694 INFO @ Sat, 15 Jan 2022 19:13:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:48: #1 tags after filtering in treatment: 8577085 INFO @ Sat, 15 Jan 2022 19:13:48: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:13:48: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:13:50: 40000000 INFO @ Sat, 15 Jan 2022 19:13:54: 41000000 INFO @ Sat, 15 Jan 2022 19:13:59: 42000000 INFO @ Sat, 15 Jan 2022 19:14:04: 43000000 INFO @ Sat, 15 Jan 2022 19:14:09: 44000000 INFO @ Sat, 15 Jan 2022 19:14:14: 45000000 INFO @ Sat, 15 Jan 2022 19:14:19: 46000000 INFO @ Sat, 15 Jan 2022 19:14:23: 47000000 INFO @ Sat, 15 Jan 2022 19:14:28: 48000000 INFO @ Sat, 15 Jan 2022 19:14:33: 49000000 INFO @ Sat, 15 Jan 2022 19:14:38: 50000000 INFO @ Sat, 15 Jan 2022 19:14:43: 51000000 INFO @ Sat, 15 Jan 2022 19:14:48: 52000000 INFO @ Sat, 15 Jan 2022 19:14:52: 53000000 INFO @ Sat, 15 Jan 2022 19:14:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:14:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:14:56: #1 total tags in treatment: 25474694 INFO @ Sat, 15 Jan 2022 19:14:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:56: #1 tags after filtering in treatment: 8577085 INFO @ Sat, 15 Jan 2022 19:14:56: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:14:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:14:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701692/SRX10701692.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling