Job ID = 14520190 SRX = SRX10592383 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:32:36 prefetch.2.10.7: 1) Downloading 'SRR14227543'... 2022-01-15T09:32:36 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:33:37 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:33:37 prefetch.2.10.7: 1) 'SRR14227543' was downloaded successfully 2022-01-15T09:33:37 prefetch.2.10.7: 'SRR14227543' has 0 unresolved dependencies Read 21937454 spots for SRR14227543/SRR14227543.sra Written 21937454 spots for SRR14227543/SRR14227543.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:56 21937454 reads; of these: 21937454 (100.00%) were paired; of these: 1203468 (5.49%) aligned concordantly 0 times 16113622 (73.45%) aligned concordantly exactly 1 time 4620364 (21.06%) aligned concordantly >1 times ---- 1203468 pairs aligned concordantly 0 times; of these: 46461 (3.86%) aligned discordantly 1 time ---- 1157007 pairs aligned 0 times concordantly or discordantly; of these: 2314014 mates make up the pairs; of these: 1837231 (79.40%) aligned 0 times 373012 (16.12%) aligned exactly 1 time 103771 (4.48%) aligned >1 times 95.81% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 8333018 / 20776363 = 0.4011 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:23: 1000000 INFO @ Sat, 15 Jan 2022 18:56:29: 2000000 INFO @ Sat, 15 Jan 2022 18:56:35: 3000000 INFO @ Sat, 15 Jan 2022 18:56:40: 4000000 INFO @ Sat, 15 Jan 2022 18:56:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:52: 6000000 INFO @ Sat, 15 Jan 2022 18:56:55: 1000000 INFO @ Sat, 15 Jan 2022 18:56:58: 7000000 INFO @ Sat, 15 Jan 2022 18:57:02: 2000000 INFO @ Sat, 15 Jan 2022 18:57:05: 8000000 INFO @ Sat, 15 Jan 2022 18:57:10: 3000000 INFO @ Sat, 15 Jan 2022 18:57:12: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:18: 4000000 INFO @ Sat, 15 Jan 2022 18:57:19: 10000000 INFO @ Sat, 15 Jan 2022 18:57:25: 1000000 INFO @ Sat, 15 Jan 2022 18:57:26: 5000000 INFO @ Sat, 15 Jan 2022 18:57:27: 11000000 INFO @ Sat, 15 Jan 2022 18:57:33: 2000000 INFO @ Sat, 15 Jan 2022 18:57:34: 12000000 INFO @ Sat, 15 Jan 2022 18:57:34: 6000000 INFO @ Sat, 15 Jan 2022 18:57:41: 3000000 INFO @ Sat, 15 Jan 2022 18:57:41: 13000000 INFO @ Sat, 15 Jan 2022 18:57:42: 7000000 INFO @ Sat, 15 Jan 2022 18:57:48: 14000000 INFO @ Sat, 15 Jan 2022 18:57:48: 4000000 INFO @ Sat, 15 Jan 2022 18:57:50: 8000000 INFO @ Sat, 15 Jan 2022 18:57:55: 15000000 INFO @ Sat, 15 Jan 2022 18:57:56: 5000000 INFO @ Sat, 15 Jan 2022 18:57:58: 9000000 INFO @ Sat, 15 Jan 2022 18:58:03: 16000000 INFO @ Sat, 15 Jan 2022 18:58:03: 6000000 INFO @ Sat, 15 Jan 2022 18:58:06: 10000000 INFO @ Sat, 15 Jan 2022 18:58:11: 17000000 INFO @ Sat, 15 Jan 2022 18:58:11: 7000000 INFO @ Sat, 15 Jan 2022 18:58:13: 11000000 INFO @ Sat, 15 Jan 2022 18:58:18: 18000000 INFO @ Sat, 15 Jan 2022 18:58:19: 8000000 INFO @ Sat, 15 Jan 2022 18:58:21: 12000000 INFO @ Sat, 15 Jan 2022 18:58:25: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:58:27: 9000000 INFO @ Sat, 15 Jan 2022 18:58:29: 13000000 INFO @ Sat, 15 Jan 2022 18:58:33: 20000000 INFO @ Sat, 15 Jan 2022 18:58:34: 10000000 INFO @ Sat, 15 Jan 2022 18:58:37: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:58:40: 21000000 INFO @ Sat, 15 Jan 2022 18:58:42: 11000000 INFO @ Sat, 15 Jan 2022 18:58:45: 15000000 INFO @ Sat, 15 Jan 2022 18:58:47: 22000000 INFO @ Sat, 15 Jan 2022 18:58:50: 12000000 INFO @ Sat, 15 Jan 2022 18:58:53: 16000000 INFO @ Sat, 15 Jan 2022 18:58:54: 23000000 INFO @ Sat, 15 Jan 2022 18:58:58: 13000000 INFO @ Sat, 15 Jan 2022 18:59:00: 17000000 INFO @ Sat, 15 Jan 2022 18:59:02: 24000000 INFO @ Sat, 15 Jan 2022 18:59:06: 14000000 INFO @ Sat, 15 Jan 2022 18:59:08: 18000000 INFO @ Sat, 15 Jan 2022 18:59:09: 25000000 INFO @ Sat, 15 Jan 2022 18:59:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:12: #1 total tags in treatment: 12417549 INFO @ Sat, 15 Jan 2022 18:59:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:12: #1 tags after filtering in treatment: 8288933 INFO @ Sat, 15 Jan 2022 18:59:12: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 18:59:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:14: 15000000 INFO @ Sat, 15 Jan 2022 18:59:16: 19000000 INFO @ Sat, 15 Jan 2022 18:59:21: 16000000 INFO @ Sat, 15 Jan 2022 18:59:24: 20000000 INFO @ Sat, 15 Jan 2022 18:59:29: 17000000 INFO @ Sat, 15 Jan 2022 18:59:32: 21000000 INFO @ Sat, 15 Jan 2022 18:59:37: 18000000 INFO @ Sat, 15 Jan 2022 18:59:40: 22000000 INFO @ Sat, 15 Jan 2022 18:59:45: 19000000 INFO @ Sat, 15 Jan 2022 18:59:48: 23000000 INFO @ Sat, 15 Jan 2022 18:59:52: 20000000 INFO @ Sat, 15 Jan 2022 18:59:55: 24000000 INFO @ Sat, 15 Jan 2022 19:00:00: 21000000 INFO @ Sat, 15 Jan 2022 19:00:03: 25000000 INFO @ Sat, 15 Jan 2022 19:00:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:06: #1 total tags in treatment: 12417549 INFO @ Sat, 15 Jan 2022 19:00:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:06: #1 tags after filtering in treatment: 8288933 INFO @ Sat, 15 Jan 2022 19:00:06: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:00:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:00:07: 22000000 INFO @ Sat, 15 Jan 2022 19:00:14: 23000000 INFO @ Sat, 15 Jan 2022 19:00:21: 24000000 INFO @ Sat, 15 Jan 2022 19:00:28: 25000000 INFO @ Sat, 15 Jan 2022 19:00:30: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:30: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:30: #1 total tags in treatment: 12417549 INFO @ Sat, 15 Jan 2022 19:00:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:31: #1 tags after filtering in treatment: 8288933 INFO @ Sat, 15 Jan 2022 19:00:31: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:00:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592383/SRX10592383.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling