Job ID = 14519949 SRX = SRX10341704 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24046115 spots for SRR13963712/SRR13963712.sra Written 24046115 spots for SRR13963712/SRR13963712.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:47 24046115 reads; of these: 24046115 (100.00%) were unpaired; of these: 732608 (3.05%) aligned 0 times 19142520 (79.61%) aligned exactly 1 time 4170987 (17.35%) aligned >1 times 96.95% overall alignment rate Time searching: 00:04:47 Overall time: 00:04:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10903966 / 23313507 = 0.4677 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:21:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:21:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:21:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:21:06: 1000000 INFO @ Sat, 15 Jan 2022 18:21:12: 2000000 INFO @ Sat, 15 Jan 2022 18:21:18: 3000000 INFO @ Sat, 15 Jan 2022 18:21:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:21:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:21:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:21:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:21:30: 5000000 INFO @ Sat, 15 Jan 2022 18:21:36: 1000000 INFO @ Sat, 15 Jan 2022 18:21:37: 6000000 INFO @ Sat, 15 Jan 2022 18:21:43: 2000000 INFO @ Sat, 15 Jan 2022 18:21:44: 7000000 INFO @ Sat, 15 Jan 2022 18:21:49: 3000000 INFO @ Sat, 15 Jan 2022 18:21:50: 8000000 INFO @ Sat, 15 Jan 2022 18:21:56: 4000000 INFO @ Sat, 15 Jan 2022 18:21:57: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:22:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:22:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:22:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:22:03: 5000000 INFO @ Sat, 15 Jan 2022 18:22:04: 10000000 INFO @ Sat, 15 Jan 2022 18:22:08: 1000000 INFO @ Sat, 15 Jan 2022 18:22:10: 6000000 INFO @ Sat, 15 Jan 2022 18:22:11: 11000000 INFO @ Sat, 15 Jan 2022 18:22:17: 2000000 INFO @ Sat, 15 Jan 2022 18:22:17: 7000000 INFO @ Sat, 15 Jan 2022 18:22:18: 12000000 INFO @ Sat, 15 Jan 2022 18:22:21: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:22:21: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:22:21: #1 total tags in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:22:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:21: #1 tags after filtering in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:22:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:21: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:22:24: 8000000 INFO @ Sat, 15 Jan 2022 18:22:25: 3000000 INFO @ Sat, 15 Jan 2022 18:22:31: 9000000 INFO @ Sat, 15 Jan 2022 18:22:31: 4000000 INFO @ Sat, 15 Jan 2022 18:22:37: 10000000 INFO @ Sat, 15 Jan 2022 18:22:38: 5000000 INFO @ Sat, 15 Jan 2022 18:22:44: 11000000 INFO @ Sat, 15 Jan 2022 18:22:45: 6000000 BedGraph に変換しました。 INFO @ Sat, 15 Jan 2022 18:22:51: 12000000 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:22:52: 7000000 INFO @ Sat, 15 Jan 2022 18:22:53: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:22:53: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:22:53: #1 total tags in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:22:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:54: #1 tags after filtering in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:22:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:54: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:22:58: 8000000 INFO @ Sat, 15 Jan 2022 18:23:04: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:23:10: 10000000 INFO @ Sat, 15 Jan 2022 18:23:16: 11000000 INFO @ Sat, 15 Jan 2022 18:23:21: 12000000 INFO @ Sat, 15 Jan 2022 18:23:24: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:23:24: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:23:24: #1 total tags in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:23:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:23:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:23:24: #1 tags after filtering in treatment: 12409541 INFO @ Sat, 15 Jan 2022 18:23:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:23:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:23:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:23:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:23:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:23:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:23:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341704/SRX10341704.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling