Job ID = 14519948 SRX = SRX10341703 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26078560 spots for SRR13963711/SRR13963711.sra Written 26078560 spots for SRR13963711/SRR13963711.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:35 26078560 reads; of these: 26078560 (100.00%) were unpaired; of these: 1669938 (6.40%) aligned 0 times 21383476 (82.00%) aligned exactly 1 time 3025146 (11.60%) aligned >1 times 93.60% overall alignment rate Time searching: 00:06:36 Overall time: 00:06:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12887665 / 24408622 = 0.5280 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:26: 1000000 INFO @ Sat, 15 Jan 2022 18:24:38: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:48: 3000000 INFO @ Sat, 15 Jan 2022 18:24:55: 1000000 INFO @ Sat, 15 Jan 2022 18:24:58: 4000000 INFO @ Sat, 15 Jan 2022 18:25:06: 2000000 INFO @ Sat, 15 Jan 2022 18:25:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:16: 6000000 INFO @ Sat, 15 Jan 2022 18:25:17: 3000000 INFO @ Sat, 15 Jan 2022 18:25:25: 1000000 INFO @ Sat, 15 Jan 2022 18:25:25: 7000000 INFO @ Sat, 15 Jan 2022 18:25:28: 4000000 INFO @ Sat, 15 Jan 2022 18:25:36: 8000000 INFO @ Sat, 15 Jan 2022 18:25:37: 2000000 INFO @ Sat, 15 Jan 2022 18:25:38: 5000000 INFO @ Sat, 15 Jan 2022 18:25:47: 9000000 INFO @ Sat, 15 Jan 2022 18:25:48: 3000000 INFO @ Sat, 15 Jan 2022 18:25:49: 6000000 INFO @ Sat, 15 Jan 2022 18:25:57: 10000000 INFO @ Sat, 15 Jan 2022 18:25:59: 4000000 INFO @ Sat, 15 Jan 2022 18:26:00: 7000000 INFO @ Sat, 15 Jan 2022 18:26:08: 11000000 INFO @ Sat, 15 Jan 2022 18:26:10: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:26:11: 8000000 INFO @ Sat, 15 Jan 2022 18:26:14: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:26:14: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:26:14: #1 total tags in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:26:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:14: #1 tags after filtering in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:26:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:21: 6000000 INFO @ Sat, 15 Jan 2022 18:26:21: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:26:31: 10000000 INFO @ Sat, 15 Jan 2022 18:26:32: 7000000 INFO @ Sat, 15 Jan 2022 18:26:42: 11000000 INFO @ Sat, 15 Jan 2022 18:26:43: 8000000 INFO @ Sat, 15 Jan 2022 18:26:47: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:26:47: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:26:47: #1 total tags in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:26:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:47: #1 tags after filtering in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:26:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:52: 9000000 INFO @ Sat, 15 Jan 2022 18:27:01: 10000000 INFO @ Sat, 15 Jan 2022 18:27:11: 11000000 INFO @ Sat, 15 Jan 2022 18:27:15: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:27:15: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:27:15: #1 total tags in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:27:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:27:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:27:16: #1 tags after filtering in treatment: 11520957 INFO @ Sat, 15 Jan 2022 18:27:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:27:16: #1 finished! INFO @ Sat, 15 Jan 2022 18:27:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:27:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:27:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:27:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:27:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341703/SRX10341703.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling