Job ID = 14519929 SRX = SRX10101444 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7924057 spots for SRR13712735/SRR13712735.sra Written 7924057 spots for SRR13712735/SRR13712735.sra Read 8224542 spots for SRR13712736/SRR13712736.sra Written 8224542 spots for SRR13712736/SRR13712736.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:03 16148599 reads; of these: 16148599 (100.00%) were unpaired; of these: 596226 (3.69%) aligned 0 times 12777119 (79.12%) aligned exactly 1 time 2775254 (17.19%) aligned >1 times 96.31% overall alignment rate Time searching: 00:03:03 Overall time: 00:03:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6075483 / 15552373 = 0.3906 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:10:47: 1000000 INFO @ Sat, 15 Jan 2022 18:10:54: 2000000 INFO @ Sat, 15 Jan 2022 18:11:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:09: 4000000 INFO @ Sat, 15 Jan 2022 18:11:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:17: 5000000 INFO @ Sat, 15 Jan 2022 18:11:18: 1000000 INFO @ Sat, 15 Jan 2022 18:11:24: 6000000 INFO @ Sat, 15 Jan 2022 18:11:27: 2000000 INFO @ Sat, 15 Jan 2022 18:11:32: 7000000 INFO @ Sat, 15 Jan 2022 18:11:36: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:39: 8000000 INFO @ Sat, 15 Jan 2022 18:11:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:45: 4000000 INFO @ Sat, 15 Jan 2022 18:11:47: 9000000 INFO @ Sat, 15 Jan 2022 18:11:48: 1000000 INFO @ Sat, 15 Jan 2022 18:11:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:11:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:11:51: #1 total tags in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:11:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:11:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:11:51: #1 tags after filtering in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:11:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:11:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:11:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:11:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:11:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:11:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:11:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:11:54: 5000000 INFO @ Sat, 15 Jan 2022 18:11:55: 2000000 INFO @ Sat, 15 Jan 2022 18:12:03: 6000000 INFO @ Sat, 15 Jan 2022 18:12:03: 3000000 INFO @ Sat, 15 Jan 2022 18:12:10: 4000000 INFO @ Sat, 15 Jan 2022 18:12:11: 7000000 INFO @ Sat, 15 Jan 2022 18:12:18: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:12:20: 8000000 INFO @ Sat, 15 Jan 2022 18:12:26: 6000000 INFO @ Sat, 15 Jan 2022 18:12:29: 9000000 INFO @ Sat, 15 Jan 2022 18:12:33: 7000000 INFO @ Sat, 15 Jan 2022 18:12:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:34: #1 total tags in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:12:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:34: #1 tags after filtering in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:12:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:12:34: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:12:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:12:41: 8000000 INFO @ Sat, 15 Jan 2022 18:12:48: 9000000 INFO @ Sat, 15 Jan 2022 18:12:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:51: #1 total tags in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:12:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:52: #1 tags after filtering in treatment: 9476890 INFO @ Sat, 15 Jan 2022 18:12:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:12:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:12:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101444/SRX10101444.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling