Job ID = 11633904 sra ファイルのダウンロード中... Completed: 31840K bytes transferred in 3 seconds (78292K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 1020525 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420518/ERR1348994.sra Written 1020525 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420518/ERR1348994.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:09 1020525 reads; of these: 1020525 (100.00%) were unpaired; of these: 244277 (23.94%) aligned 0 times 601973 (58.99%) aligned exactly 1 time 174275 (17.08%) aligned >1 times 76.06% overall alignment rate Time searching: 00:00:09 Overall time: 00:00:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 172130 / 776248 = 0.2217 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:19:30: # Command line: callpeak -t ERX1420518.bam -f BAM -g 12100000 -n ERX1420518.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420518.05 # format = BAM # ChIP-seq file = ['ERX1420518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:30: # Command line: callpeak -t ERX1420518.bam -f BAM -g 12100000 -n ERX1420518.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420518.20 # format = BAM # ChIP-seq file = ['ERX1420518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:30: # Command line: callpeak -t ERX1420518.bam -f BAM -g 12100000 -n ERX1420518.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420518.10 # format = BAM # ChIP-seq file = ['ERX1420518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:34: #1 total tags in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:34: #1 total tags in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:34: #1 tags after filtering in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:34: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:34: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:34: #1 total tags in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:34: #1 tags after filtering in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:34: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #1 tags after filtering in treatment: 604118 INFO @ Fri, 15 Feb 2019 09:19:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:34: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #2 number of paired peaks: 108 WARNING @ Fri, 15 Feb 2019 09:19:34: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:34: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:34: #2 number of paired peaks: 108 WARNING @ Fri, 15 Feb 2019 09:19:34: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:34: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:34: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:34: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:34: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:34: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 predicted fragment length is 193 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2 alternative fragment length(s) may be 3,193,206 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2.2 Generate R script for model : ERX1420518.20_model.r INFO @ Fri, 15 Feb 2019 09:19:34: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:34: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 predicted fragment length is 193 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2 alternative fragment length(s) may be 3,193,206 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2.2 Generate R script for model : ERX1420518.05_model.r INFO @ Fri, 15 Feb 2019 09:19:34: #2 number of paired peaks: 108 WARNING @ Fri, 15 Feb 2019 09:19:34: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:34: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:34: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:34: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:34: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:34: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:34: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:34: #2 predicted fragment length is 193 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2 alternative fragment length(s) may be 3,193,206 bps INFO @ Fri, 15 Feb 2019 09:19:34: #2.2 Generate R script for model : ERX1420518.10_model.r INFO @ Fri, 15 Feb 2019 09:19:34: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:36: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:36: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:36: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write output xls file... ERX1420518.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write output xls file... ERX1420518.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write peak in narrowPeak format file... ERX1420518.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write peak in narrowPeak format file... ERX1420518.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write summits bed file... ERX1420518.05_summits.bed INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write summits bed file... ERX1420518.10_summits.bed INFO @ Fri, 15 Feb 2019 09:19:37: Done! INFO @ Fri, 15 Feb 2019 09:19:37: Done! INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write output xls file... ERX1420518.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write peak in narrowPeak format file... ERX1420518.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:37: #4 Write summits bed file... ERX1420518.20_summits.bed INFO @ Fri, 15 Feb 2019 09:19:37: Done! pass1 - making usageList (16 chroms): 4 millis pass2 - checking and writing primary data (451 records, 4 fields): 7 millis pass1 - making usageList (16 chroms): 11 millis pass2 - checking and writing primary data (1292 records, 4 fields): 13 millis CompletedMACS2peakCalling CompletedMACS2peakCalling pass1 - making usageList (15 chroms): 13 millis pass2 - checking and writing primary data (75 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。