Job ID = 11633614 sra ファイルのダウンロード中... Completed: 125856K bytes transferred in 4 seconds (214913K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 1726783 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420233/ERR1348709.sra Written 1726783 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420233/ERR1348709.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:55 1726783 reads; of these: 1726783 (100.00%) were paired; of these: 1485233 (86.01%) aligned concordantly 0 times 203507 (11.79%) aligned concordantly exactly 1 time 38043 (2.20%) aligned concordantly >1 times ---- 1485233 pairs aligned concordantly 0 times; of these: 288843 (19.45%) aligned discordantly 1 time ---- 1196390 pairs aligned 0 times concordantly or discordantly; of these: 2392780 mates make up the pairs; of these: 2099056 (87.72%) aligned 0 times 200349 (8.37%) aligned exactly 1 time 93375 (3.90%) aligned >1 times 39.22% overall alignment rate Time searching: 00:00:56 Overall time: 00:00:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 112261 / 325015 = 0.3454 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:05:23: # Command line: callpeak -t ERX1420233.bam -f BAM -g 12100000 -n ERX1420233.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420233.05 # format = BAM # ChIP-seq file = ['ERX1420233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:23: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:23: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:23: # Command line: callpeak -t ERX1420233.bam -f BAM -g 12100000 -n ERX1420233.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420233.10 # format = BAM # ChIP-seq file = ['ERX1420233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:23: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:23: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:23: # Command line: callpeak -t ERX1420233.bam -f BAM -g 12100000 -n ERX1420233.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420233.20 # format = BAM # ChIP-seq file = ['ERX1420233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:23: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:23: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:29: 1000000 INFO @ Fri, 15 Feb 2019 09:05:29: 1000000 INFO @ Fri, 15 Feb 2019 09:05:29: 1000000 INFO @ Fri, 15 Feb 2019 09:05:29: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:05:29: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:05:29: #1 total tags in treatment: 157826 INFO @ Fri, 15 Feb 2019 09:05:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:05:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:05:29: #1 tags after filtering in treatment: 151038 INFO @ Fri, 15 Feb 2019 09:05:29: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 15 Feb 2019 09:05:29: #1 finished! INFO @ Fri, 15 Feb 2019 09:05:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:05:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:05:29: #2 number of paired peaks: 165 WARNING @ Fri, 15 Feb 2019 09:05:29: Fewer paired peaks (165) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 165 pairs to build model! INFO @ Fri, 15 Feb 2019 09:05:29: start model_add_line... INFO @ Fri, 15 Feb 2019 09:05:29: start X-correlation... INFO @ Fri, 15 Feb 2019 09:05:29: end of X-cor INFO @ Fri, 15 Feb 2019 09:05:29: #2 finished! INFO @ Fri, 15 Feb 2019 09:05:29: #2 predicted fragment length is 296 bps INFO @ Fri, 15 Feb 2019 09:05:29: #2 alternative fragment length(s) may be 4,296 bps INFO @ Fri, 15 Feb 2019 09:05:29: #2.2 Generate R script for model : ERX1420233.20_model.r INFO @ Fri, 15 Feb 2019 09:05:29: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:05:29: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:05:29: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:05:29: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:05:29: #1 total tags in treatment: 157826 INFO @ Fri, 15 Feb 2019 09:05:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:05:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:05:29: #1 tags after filtering in treatment: 151038 INFO @ Fri, 15 Feb 2019 09:05:29: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 15 Feb 2019 09:05:29: #1 finished! INFO @ Fri, 15 Feb 2019 09:05:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:05:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:05:30: #2 number of paired peaks: 165 WARNING @ Fri, 15 Feb 2019 09:05:30: Fewer paired peaks (165) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 165 pairs to build model! INFO @ Fri, 15 Feb 2019 09:05:30: start model_add_line... INFO @ Fri, 15 Feb 2019 09:05:30: start X-correlation... INFO @ Fri, 15 Feb 2019 09:05:30: end of X-cor INFO @ Fri, 15 Feb 2019 09:05:30: #2 finished! INFO @ Fri, 15 Feb 2019 09:05:30: #2 predicted fragment length is 296 bps INFO @ Fri, 15 Feb 2019 09:05:30: #2 alternative fragment length(s) may be 4,296 bps INFO @ Fri, 15 Feb 2019 09:05:30: #2.2 Generate R script for model : ERX1420233.05_model.r INFO @ Fri, 15 Feb 2019 09:05:30: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:05:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:05:30: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:05:30: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:05:30: #1 total tags in treatment: 157826 INFO @ Fri, 15 Feb 2019 09:05:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:05:30: #1 tags after filtering in treatment: 151038 INFO @ Fri, 15 Feb 2019 09:05:30: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 15 Feb 2019 09:05:30: #1 finished! INFO @ Fri, 15 Feb 2019 09:05:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:05:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:05:30: #2 number of paired peaks: 165 WARNING @ Fri, 15 Feb 2019 09:05:30: Fewer paired peaks (165) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 165 pairs to build model! INFO @ Fri, 15 Feb 2019 09:05:30: start model_add_line... INFO @ Fri, 15 Feb 2019 09:05:30: start X-correlation... INFO @ Fri, 15 Feb 2019 09:05:30: end of X-cor INFO @ Fri, 15 Feb 2019 09:05:30: #2 finished! INFO @ Fri, 15 Feb 2019 09:05:30: #2 predicted fragment length is 296 bps INFO @ Fri, 15 Feb 2019 09:05:30: #2 alternative fragment length(s) may be 4,296 bps INFO @ Fri, 15 Feb 2019 09:05:30: #2.2 Generate R script for model : ERX1420233.10_model.r INFO @ Fri, 15 Feb 2019 09:05:30: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:05:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:05:30: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write output xls file... ERX1420233.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write peak in narrowPeak format file... ERX1420233.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write summits bed file... ERX1420233.20_summits.bed INFO @ Fri, 15 Feb 2019 09:05:30: Done! INFO @ Fri, 15 Feb 2019 09:05:30: #3 Call peaks for each chromosome... pass1 - making usageList (10 chroms): 7 millis pass2 - checking and writing primary data (13 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write output xls file... ERX1420233.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write peak in narrowPeak format file... ERX1420233.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:05:30: #4 Write summits bed file... ERX1420233.05_summits.bed INFO @ Fri, 15 Feb 2019 09:05:30: Done! pass1 - making usageList (16 chroms): 6 millis pass2 - checking and writing primary data (103 records, 4 fields): 4 millis INFO @ Fri, 15 Feb 2019 09:05:30: #3 Call peaks for each chromosome... CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:05:31: #4 Write output xls file... ERX1420233.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:05:31: #4 Write peak in narrowPeak format file... ERX1420233.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:05:31: #4 Write summits bed file... ERX1420233.10_summits.bed INFO @ Fri, 15 Feb 2019 09:05:31: Done! pass1 - making usageList (14 chroms): 6 millis pass2 - checking and writing primary data (58 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。