Job ID = 14518267 SRX = SRX8975480 Genome = rn6 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3023 spots for SRR12481604/SRR12481604.sra Written 3023 spots for SRR12481604/SRR12481604.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:02 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:00 3023 reads; of these: 3023 (100.00%) were paired; of these: 321 (10.62%) aligned concordantly 0 times 2101 (69.50%) aligned concordantly exactly 1 time 601 (19.88%) aligned concordantly >1 times ---- 321 pairs aligned concordantly 0 times; of these: 41 (12.77%) aligned discordantly 1 time ---- 280 pairs aligned 0 times concordantly or discordantly; of these: 560 mates make up the pairs; of these: 301 (53.75%) aligned 0 times 161 (28.75%) aligned exactly 1 time 98 (17.50%) aligned >1 times 95.02% overall alignment rate Time searching: 00:00:05 Overall time: 00:00:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_rmdup_core] processing reference chr1... [bam_rmdup_core] processing reference chr10... [bam_rmdup_core] processing reference chr11... [bam_rmdup_core] processing reference chr12... [bam_rmdup_core] processing reference chr13... [bam_rmdup_core] processing reference chr14... [bam_rmdup_core] processing reference chr15... [bam_rmdup_core] processing reference chr16... [bam_rmdup_core] processing reference chr17... [bam_rmdup_core] processing reference chr18... [bam_rmdup_core] processing reference chr19... [bam_rmdup_core] processing reference chr2... [bam_rmdup_core] processing reference chr20... [bam_rmdup_core] processing reference chr3... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chr5... [bam_rmdup_core] processing reference chr6... [bam_rmdup_core] processing reference chr7... [bam_rmdup_core] processing reference chr8... [bam_rmdup_core] processing reference chr9... [bam_rmdup_core] processing reference chrUn_KL568408v1... [bam_rmdup_core] processing reference chrUn_KL568413v1... [bam_rmdup_core] processing reference chrUn_AABR07024102v1... [bam_rmdup_core] processing reference chrUn_KL568432v1... [bam_rmdup_core] processing reference chrUn_AABR07024145v1... [bam_rmdup_core] processing reference chrUn_AABR07024203v1... [bam_rmdup_core] processing reference chrUn_AABR07024351v1... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrY_KL568139v1_random... [bam_rmdup_core] processing reference chrY_KL568140v1_random... [bam_rmdup_core] processing reference chrY_KL568141v1_random... [bam_rmdup_core] processing reference chrY_KL568148v1_random... [bam_rmdup_core] processing reference chrY_KL568149v1_random... [bam_rmdup_core] processing reference chrY_KL568151v1_random... [bam_rmdup_core] processing reference chrY_KL568155v1_random... [bam_rmdup_core] processing reference chrY_KL568156v1_random... [bam_rmdup_core] processing reference chrY_KL568157v1_random... [bam_rmdup_core] processing reference chrY_KL568159v1_random... [bam_rmdup_core] processing reference chrY_KL568161v1_random... [bam_rmdup_core] processing reference chrY_KL568162v1_random... [bam_rmdup_core] processing reference chrY_KL568163v1_random... [bam_rmdup_core] processing reference chrY_KL568164v1_random... [bam_rmdup_core] 4 / 2741 = 0.0015 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 11:33:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 11:33:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 11:33:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 11:33:22: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 11:33:22: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 11:33:22: #1 total tags in treatment: 2698 INFO @ Sat, 15 Jan 2022 11:33:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 11:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 11:33:22: #1 tags after filtering in treatment: 2679 INFO @ Sat, 15 Jan 2022 11:33:22: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 15 Jan 2022 11:33:22: #1 finished! INFO @ Sat, 15 Jan 2022 11:33:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 11:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 11:33:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 11:33:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 11:33:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 11:33:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 11:33:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 11:33:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 11:33:50: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 11:33:50: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 11:33:50: #1 total tags in treatment: 2698 INFO @ Sat, 15 Jan 2022 11:33:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 11:33:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 11:33:50: #1 tags after filtering in treatment: 2679 INFO @ Sat, 15 Jan 2022 11:33:50: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 15 Jan 2022 11:33:50: #1 finished! INFO @ Sat, 15 Jan 2022 11:33:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 11:33:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 11:33:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 11:33:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 11:33:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 11:34:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 11:34:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 11:34:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 11:34:19: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 11:34:19: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 11:34:19: #1 total tags in treatment: 2698 INFO @ Sat, 15 Jan 2022 11:34:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 11:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 11:34:19: #1 tags after filtering in treatment: 2679 INFO @ Sat, 15 Jan 2022 11:34:19: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 15 Jan 2022 11:34:19: #1 finished! INFO @ Sat, 15 Jan 2022 11:34:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 11:34:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 11:34:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 11:34:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 11:34:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/rn6/SRX8975480/SRX8975480.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。