Job ID = 14517857 SRX = SRX6581752 Genome = rn6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24743774 spots for SRR9825085/SRR9825085.sra Written 24743774 spots for SRR9825085/SRR9825085.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:02 Multiseed full-index search: 00:29:04 24743774 reads; of these: 24743774 (100.00%) were unpaired; of these: 671185 (2.71%) aligned 0 times 17536625 (70.87%) aligned exactly 1 time 6535964 (26.41%) aligned >1 times 97.29% overall alignment rate Time searching: 00:29:08 Overall time: 00:29:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2360812 / 24072589 = 0.0981 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 06:09:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 06:09:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 06:09:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 06:09:48: 1000000 INFO @ Sat, 15 Jan 2022 06:09:55: 2000000 INFO @ Sat, 15 Jan 2022 06:10:01: 3000000 INFO @ Sat, 15 Jan 2022 06:10:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 06:10:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 06:10:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 06:10:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 06:10:14: 5000000 INFO @ Sat, 15 Jan 2022 06:10:18: 1000000 INFO @ Sat, 15 Jan 2022 06:10:21: 6000000 INFO @ Sat, 15 Jan 2022 06:10:25: 2000000 INFO @ Sat, 15 Jan 2022 06:10:27: 7000000 INFO @ Sat, 15 Jan 2022 06:10:32: 3000000 INFO @ Sat, 15 Jan 2022 06:10:34: 8000000 INFO @ Sat, 15 Jan 2022 06:10:39: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 06:10:40: 9000000 INFO @ Sat, 15 Jan 2022 06:10:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 06:10:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 06:10:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 06:10:46: 5000000 INFO @ Sat, 15 Jan 2022 06:10:47: 10000000 INFO @ Sat, 15 Jan 2022 06:10:49: 1000000 INFO @ Sat, 15 Jan 2022 06:10:52: 6000000 INFO @ Sat, 15 Jan 2022 06:10:53: 11000000 INFO @ Sat, 15 Jan 2022 06:10:56: 2000000 INFO @ Sat, 15 Jan 2022 06:10:59: 7000000 INFO @ Sat, 15 Jan 2022 06:11:00: 12000000 INFO @ Sat, 15 Jan 2022 06:11:03: 3000000 INFO @ Sat, 15 Jan 2022 06:11:05: 8000000 INFO @ Sat, 15 Jan 2022 06:11:06: 13000000 INFO @ Sat, 15 Jan 2022 06:11:10: 4000000 INFO @ Sat, 15 Jan 2022 06:11:12: 9000000 INFO @ Sat, 15 Jan 2022 06:11:13: 14000000 INFO @ Sat, 15 Jan 2022 06:11:17: 5000000 INFO @ Sat, 15 Jan 2022 06:11:18: 10000000 INFO @ Sat, 15 Jan 2022 06:11:20: 15000000 INFO @ Sat, 15 Jan 2022 06:11:23: 6000000 INFO @ Sat, 15 Jan 2022 06:11:25: 11000000 INFO @ Sat, 15 Jan 2022 06:11:26: 16000000 INFO @ Sat, 15 Jan 2022 06:11:30: 7000000 INFO @ Sat, 15 Jan 2022 06:11:31: 12000000 INFO @ Sat, 15 Jan 2022 06:11:33: 17000000 INFO @ Sat, 15 Jan 2022 06:11:37: 8000000 INFO @ Sat, 15 Jan 2022 06:11:37: 13000000 INFO @ Sat, 15 Jan 2022 06:11:39: 18000000 INFO @ Sat, 15 Jan 2022 06:11:43: 9000000 INFO @ Sat, 15 Jan 2022 06:11:44: 14000000 INFO @ Sat, 15 Jan 2022 06:11:46: 19000000 INFO @ Sat, 15 Jan 2022 06:11:50: 10000000 INFO @ Sat, 15 Jan 2022 06:11:51: 15000000 INFO @ Sat, 15 Jan 2022 06:11:52: 20000000 INFO @ Sat, 15 Jan 2022 06:11:57: 11000000 INFO @ Sat, 15 Jan 2022 06:11:57: 16000000 INFO @ Sat, 15 Jan 2022 06:11:59: 21000000 INFO @ Sat, 15 Jan 2022 06:12:03: 12000000 INFO @ Sat, 15 Jan 2022 06:12:03: 17000000 INFO @ Sat, 15 Jan 2022 06:12:04: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 06:12:04: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 06:12:04: #1 total tags in treatment: 21711777 INFO @ Sat, 15 Jan 2022 06:12:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 06:12:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 06:12:04: #1 tags after filtering in treatment: 21711653 INFO @ Sat, 15 Jan 2022 06:12:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 06:12:04: #1 finished! INFO @ Sat, 15 Jan 2022 06:12:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 06:12:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 06:12:07: #2 number of paired peaks: 22720 INFO @ Sat, 15 Jan 2022 06:12:07: start model_add_line... INFO @ Sat, 15 Jan 2022 06:12:07: start X-correlation... INFO @ Sat, 15 Jan 2022 06:12:07: end of X-cor INFO @ Sat, 15 Jan 2022 06:12:07: #2 finished! INFO @ Sat, 15 Jan 2022 06:12:07: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Jan 2022 06:12:07: #2 alternative fragment length(s) may be 175 bps INFO @ Sat, 15 Jan 2022 06:12:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05_model.r INFO @ Sat, 15 Jan 2022 06:12:07: #3 Call peaks... INFO @ Sat, 15 Jan 2022 06:12:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 06:12:10: 13000000 INFO @ Sat, 15 Jan 2022 06:12:10: 18000000 INFO @ Sat, 15 Jan 2022 06:12:16: 19000000 INFO @ Sat, 15 Jan 2022 06:12:16: 14000000 INFO @ Sat, 15 Jan 2022 06:12:23: 20000000 INFO @ Sat, 15 Jan 2022 06:12:23: 15000000 INFO @ Sat, 15 Jan 2022 06:12:29: 21000000 INFO @ Sat, 15 Jan 2022 06:12:30: 16000000 INFO @ Sat, 15 Jan 2022 06:12:34: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 06:12:34: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 06:12:34: #1 total tags in treatment: 21711777 INFO @ Sat, 15 Jan 2022 06:12:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 06:12:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 06:12:35: #1 tags after filtering in treatment: 21711653 INFO @ Sat, 15 Jan 2022 06:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 06:12:35: #1 finished! INFO @ Sat, 15 Jan 2022 06:12:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 06:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 06:12:36: 17000000 INFO @ Sat, 15 Jan 2022 06:12:37: #2 number of paired peaks: 22720 INFO @ Sat, 15 Jan 2022 06:12:37: start model_add_line... INFO @ Sat, 15 Jan 2022 06:12:37: start X-correlation... INFO @ Sat, 15 Jan 2022 06:12:37: end of X-cor INFO @ Sat, 15 Jan 2022 06:12:37: #2 finished! INFO @ Sat, 15 Jan 2022 06:12:37: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Jan 2022 06:12:37: #2 alternative fragment length(s) may be 175 bps INFO @ Sat, 15 Jan 2022 06:12:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10_model.r INFO @ Sat, 15 Jan 2022 06:12:37: #3 Call peaks... INFO @ Sat, 15 Jan 2022 06:12:37: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 06:12:42: 18000000 INFO @ Sat, 15 Jan 2022 06:12:49: 19000000 INFO @ Sat, 15 Jan 2022 06:12:54: 20000000 INFO @ Sat, 15 Jan 2022 06:12:56: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 06:13:00: 21000000 INFO @ Sat, 15 Jan 2022 06:13:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 06:13:05: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 06:13:05: #1 total tags in treatment: 21711777 INFO @ Sat, 15 Jan 2022 06:13:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 06:13:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 06:13:05: #1 tags after filtering in treatment: 21711653 INFO @ Sat, 15 Jan 2022 06:13:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 06:13:05: #1 finished! INFO @ Sat, 15 Jan 2022 06:13:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 06:13:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 06:13:08: #2 number of paired peaks: 22720 INFO @ Sat, 15 Jan 2022 06:13:08: start model_add_line... INFO @ Sat, 15 Jan 2022 06:13:08: start X-correlation... INFO @ Sat, 15 Jan 2022 06:13:08: end of X-cor INFO @ Sat, 15 Jan 2022 06:13:08: #2 finished! INFO @ Sat, 15 Jan 2022 06:13:08: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Jan 2022 06:13:08: #2 alternative fragment length(s) may be 175 bps INFO @ Sat, 15 Jan 2022 06:13:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20_model.r INFO @ Sat, 15 Jan 2022 06:13:08: #3 Call peaks... INFO @ Sat, 15 Jan 2022 06:13:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 06:13:22: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05_peaks.xls INFO @ Sat, 15 Jan 2022 06:13:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 06:13:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.05_summits.bed INFO @ Sat, 15 Jan 2022 06:13:22: Done! pass1 - making usageList (81 chroms): 3 millis pass2 - checking and writing primary data (17794 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 06:13:25: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 06:13:50: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10_peaks.xls INFO @ Sat, 15 Jan 2022 06:13:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 06:13:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.10_summits.bed INFO @ Sat, 15 Jan 2022 06:13:50: Done! pass1 - making usageList (69 chroms): 3 millis pass2 - checking and writing primary data (10480 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 06:13:57: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 06:14:23: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20_peaks.xls INFO @ Sat, 15 Jan 2022 06:14:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 06:14:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX6581752/SRX6581752.20_summits.bed INFO @ Sat, 15 Jan 2022 06:14:23: Done! pass1 - making usageList (49 chroms): 2 millis pass2 - checking and writing primary data (5199 records, 4 fields): 7 millis CompletedMACS2peakCalling