Job ID = 2640423 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-08-24T07:35:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:35:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:35:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:44:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:44:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:44:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:45:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:47:30 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:47:30 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:48:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:51:26 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:54:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:57:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:00:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:07:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:08:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:09:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:14:40 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:18:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:22:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:22:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:24:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:25:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:28:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:45:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:46:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:11:44 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:12:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:19:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:20:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:29:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T09:31:23 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 222,940,519 reads read : 222,940,519 reads written : 222,940,519 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:02 Time loading mirror index: 00:00:01 Multiseed full-index search: 04:41:28 222940519 reads; of these: 222940519 (100.00%) were unpaired; of these: 40263021 (18.06%) aligned 0 times 145516673 (65.27%) aligned exactly 1 time 37160825 (16.67%) aligned >1 times 81.94% overall alignment rate Time searching: 04:41:32 Overall time: 04:41:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_sort_core] merging from 76 files... [bam_rmdupse_core] 147005426 / 182677498 = 0.8047 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sun, 25 Aug 2019 00:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 25 Aug 2019 00:01:45: #1 read tag files... INFO @ Sun, 25 Aug 2019 00:01:45: #1 read treatment tags... INFO @ Sun, 25 Aug 2019 00:01:53: 1000000 INFO @ Sun, 25 Aug 2019 00:02:01: 2000000 INFO @ Sun, 25 Aug 2019 00:02:08: 3000000 INFO @ Sun, 25 Aug 2019 00:02:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 25 Aug 2019 00:02:14: #1 read tag files... INFO @ Sun, 25 Aug 2019 00:02:14: #1 read treatment tags... INFO @ Sun, 25 Aug 2019 00:02:16: 4000000 INFO @ Sun, 25 Aug 2019 00:02:23: 1000000 INFO @ Sun, 25 Aug 2019 00:02:24: 5000000 INFO @ Sun, 25 Aug 2019 00:02:31: 2000000 INFO @ Sun, 25 Aug 2019 00:02:33: 6000000 INFO @ Sun, 25 Aug 2019 00:02:40: 3000000 INFO @ Sun, 25 Aug 2019 00:02:41: 7000000 BedGraph に変換中... INFO @ Sun, 25 Aug 2019 00:02:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 25 Aug 2019 00:02:44: #1 read tag files... INFO @ Sun, 25 Aug 2019 00:02:44: #1 read treatment tags... INFO @ Sun, 25 Aug 2019 00:02:48: 4000000 INFO @ Sun, 25 Aug 2019 00:02:50: 8000000 INFO @ Sun, 25 Aug 2019 00:02:54: 1000000 INFO @ Sun, 25 Aug 2019 00:02:56: 5000000 INFO @ Sun, 25 Aug 2019 00:02:59: 9000000 INFO @ Sun, 25 Aug 2019 00:03:03: 2000000 INFO @ Sun, 25 Aug 2019 00:03:05: 6000000 INFO @ Sun, 25 Aug 2019 00:03:08: 10000000 INFO @ Sun, 25 Aug 2019 00:03:13: 3000000 INFO @ Sun, 25 Aug 2019 00:03:14: 7000000 INFO @ Sun, 25 Aug 2019 00:03:16: 11000000 INFO @ Sun, 25 Aug 2019 00:03:22: 4000000 INFO @ Sun, 25 Aug 2019 00:03:23: 8000000 INFO @ Sun, 25 Aug 2019 00:03:25: 12000000 INFO @ Sun, 25 Aug 2019 00:03:31: 5000000 INFO @ Sun, 25 Aug 2019 00:03:31: 9000000 INFO @ Sun, 25 Aug 2019 00:03:34: 13000000 INFO @ Sun, 25 Aug 2019 00:03:40: 10000000 INFO @ Sun, 25 Aug 2019 00:03:40: 6000000 INFO @ Sun, 25 Aug 2019 00:03:42: 14000000 INFO @ Sun, 25 Aug 2019 00:03:49: 11000000 INFO @ Sun, 25 Aug 2019 00:03:50: 7000000 INFO @ Sun, 25 Aug 2019 00:03:51: 15000000 INFO @ Sun, 25 Aug 2019 00:03:57: 12000000 INFO @ Sun, 25 Aug 2019 00:03:59: 16000000 INFO @ Sun, 25 Aug 2019 00:03:59: 8000000 INFO @ Sun, 25 Aug 2019 00:04:06: 13000000 INFO @ Sun, 25 Aug 2019 00:04:08: 17000000 INFO @ Sun, 25 Aug 2019 00:04:09: 9000000 INFO @ Sun, 25 Aug 2019 00:04:15: 14000000 INFO @ Sun, 25 Aug 2019 00:04:17: 18000000 INFO @ Sun, 25 Aug 2019 00:04:18: 10000000 INFO @ Sun, 25 Aug 2019 00:04:23: 15000000 INFO @ Sun, 25 Aug 2019 00:04:25: 19000000 INFO @ Sun, 25 Aug 2019 00:04:28: 11000000 INFO @ Sun, 25 Aug 2019 00:04:32: 16000000 INFO @ Sun, 25 Aug 2019 00:04:34: 20000000 INFO @ Sun, 25 Aug 2019 00:04:37: 12000000 INFO @ Sun, 25 Aug 2019 00:04:41: 17000000 INFO @ Sun, 25 Aug 2019 00:04:43: 21000000 INFO @ Sun, 25 Aug 2019 00:04:46: 13000000 INFO @ Sun, 25 Aug 2019 00:04:49: 18000000 INFO @ Sun, 25 Aug 2019 00:04:52: 22000000 INFO @ Sun, 25 Aug 2019 00:04:55: 14000000 INFO @ Sun, 25 Aug 2019 00:04:58: 19000000 INFO @ Sun, 25 Aug 2019 00:05:00: 23000000 INFO @ Sun, 25 Aug 2019 00:05:05: 15000000 INFO @ Sun, 25 Aug 2019 00:05:07: 20000000 INFO @ Sun, 25 Aug 2019 00:05:09: 24000000 INFO @ Sun, 25 Aug 2019 00:05:14: 16000000 INFO @ Sun, 25 Aug 2019 00:05:15: 21000000 INFO @ Sun, 25 Aug 2019 00:05:18: 25000000 INFO @ Sun, 25 Aug 2019 00:05:23: 17000000 INFO @ Sun, 25 Aug 2019 00:05:24: 22000000 INFO @ Sun, 25 Aug 2019 00:05:26: 26000000 INFO @ Sun, 25 Aug 2019 00:05:32: 18000000 INFO @ Sun, 25 Aug 2019 00:05:33: 23000000 INFO @ Sun, 25 Aug 2019 00:05:35: 27000000 INFO @ Sun, 25 Aug 2019 00:05:41: 19000000 INFO @ Sun, 25 Aug 2019 00:05:41: 24000000 INFO @ Sun, 25 Aug 2019 00:05:44: 28000000 INFO @ Sun, 25 Aug 2019 00:05:50: 25000000 INFO @ Sun, 25 Aug 2019 00:05:50: 20000000 INFO @ Sun, 25 Aug 2019 00:05:52: 29000000 INFO @ Sun, 25 Aug 2019 00:05:59: 26000000 INFO @ Sun, 25 Aug 2019 00:06:00: 21000000 INFO @ Sun, 25 Aug 2019 00:06:01: 30000000 INFO @ Sun, 25 Aug 2019 00:06:08: 27000000 INFO @ Sun, 25 Aug 2019 00:06:09: 22000000 INFO @ Sun, 25 Aug 2019 00:06:10: 31000000 INFO @ Sun, 25 Aug 2019 00:06:16: 28000000 INFO @ Sun, 25 Aug 2019 00:06:18: 23000000 INFO @ Sun, 25 Aug 2019 00:06:19: 32000000 INFO @ Sun, 25 Aug 2019 00:06:25: 29000000 INFO @ Sun, 25 Aug 2019 00:06:27: 24000000 INFO @ Sun, 25 Aug 2019 00:06:28: 33000000 INFO @ Sun, 25 Aug 2019 00:06:34: 30000000 INFO @ Sun, 25 Aug 2019 00:06:36: 25000000 INFO @ Sun, 25 Aug 2019 00:06:36: 34000000 INFO @ Sun, 25 Aug 2019 00:06:42: 31000000 INFO @ Sun, 25 Aug 2019 00:06:45: 35000000 INFO @ Sun, 25 Aug 2019 00:06:45: 26000000 INFO @ Sun, 25 Aug 2019 00:06:51: 32000000 INFO @ Sun, 25 Aug 2019 00:06:51: #1 tag size is determined as 51 bps INFO @ Sun, 25 Aug 2019 00:06:51: #1 tag size = 51 INFO @ Sun, 25 Aug 2019 00:06:51: #1 total tags in treatment: 35672072 INFO @ Sun, 25 Aug 2019 00:06:51: #1 user defined the maximum tags... INFO @ Sun, 25 Aug 2019 00:06:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 25 Aug 2019 00:06:52: #1 tags after filtering in treatment: 35671997 INFO @ Sun, 25 Aug 2019 00:06:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 25 Aug 2019 00:06:52: #1 finished! INFO @ Sun, 25 Aug 2019 00:06:52: #2 Build Peak Model... INFO @ Sun, 25 Aug 2019 00:06:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 25 Aug 2019 00:06:54: 27000000 INFO @ Sun, 25 Aug 2019 00:06:58: #2 number of paired peaks: 39988 INFO @ Sun, 25 Aug 2019 00:06:58: start model_add_line... INFO @ Sun, 25 Aug 2019 00:06:58: start X-correlation... INFO @ Sun, 25 Aug 2019 00:06:58: end of X-cor INFO @ Sun, 25 Aug 2019 00:06:58: #2 finished! INFO @ Sun, 25 Aug 2019 00:06:58: #2 predicted fragment length is 82 bps INFO @ Sun, 25 Aug 2019 00:06:58: #2 alternative fragment length(s) may be 82 bps INFO @ Sun, 25 Aug 2019 00:06:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05_model.r WARNING @ Sun, 25 Aug 2019 00:06:58: #2 Since the d (82) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 25 Aug 2019 00:06:58: #2 You may need to consider one of the other alternative d(s): 82 WARNING @ Sun, 25 Aug 2019 00:06:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 25 Aug 2019 00:06:58: #3 Call peaks... INFO @ Sun, 25 Aug 2019 00:06:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 25 Aug 2019 00:06:59: 33000000 INFO @ Sun, 25 Aug 2019 00:07:03: 28000000 INFO @ Sun, 25 Aug 2019 00:07:07: 34000000 INFO @ Sun, 25 Aug 2019 00:07:12: 29000000 INFO @ Sun, 25 Aug 2019 00:07:14: 35000000 INFO @ Sun, 25 Aug 2019 00:07:20: #1 tag size is determined as 51 bps INFO @ Sun, 25 Aug 2019 00:07:20: #1 tag size = 51 INFO @ Sun, 25 Aug 2019 00:07:20: #1 total tags in treatment: 35672072 INFO @ Sun, 25 Aug 2019 00:07:20: #1 user defined the maximum tags... INFO @ Sun, 25 Aug 2019 00:07:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 25 Aug 2019 00:07:21: #1 tags after filtering in treatment: 35671997 INFO @ Sun, 25 Aug 2019 00:07:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 25 Aug 2019 00:07:21: #1 finished! INFO @ Sun, 25 Aug 2019 00:07:21: #2 Build Peak Model... INFO @ Sun, 25 Aug 2019 00:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 25 Aug 2019 00:07:21: 30000000 INFO @ Sun, 25 Aug 2019 00:07:27: #2 number of paired peaks: 39988 INFO @ Sun, 25 Aug 2019 00:07:27: start model_add_line... INFO @ Sun, 25 Aug 2019 00:07:27: start X-correlation... INFO @ Sun, 25 Aug 2019 00:07:27: end of X-cor INFO @ Sun, 25 Aug 2019 00:07:27: #2 finished! INFO @ Sun, 25 Aug 2019 00:07:27: #2 predicted fragment length is 82 bps INFO @ Sun, 25 Aug 2019 00:07:27: #2 alternative fragment length(s) may be 82 bps INFO @ Sun, 25 Aug 2019 00:07:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10_model.r WARNING @ Sun, 25 Aug 2019 00:07:27: #2 Since the d (82) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 25 Aug 2019 00:07:27: #2 You may need to consider one of the other alternative d(s): 82 WARNING @ Sun, 25 Aug 2019 00:07:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 25 Aug 2019 00:07:27: #3 Call peaks... INFO @ Sun, 25 Aug 2019 00:07:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 25 Aug 2019 00:07:30: 31000000 INFO @ Sun, 25 Aug 2019 00:07:39: 32000000 INFO @ Sun, 25 Aug 2019 00:07:48: 33000000 INFO @ Sun, 25 Aug 2019 00:07:57: 34000000 INFO @ Sun, 25 Aug 2019 00:08:06: 35000000 INFO @ Sun, 25 Aug 2019 00:08:12: #1 tag size is determined as 51 bps INFO @ Sun, 25 Aug 2019 00:08:12: #1 tag size = 51 INFO @ Sun, 25 Aug 2019 00:08:12: #1 total tags in treatment: 35672072 INFO @ Sun, 25 Aug 2019 00:08:12: #1 user defined the maximum tags... INFO @ Sun, 25 Aug 2019 00:08:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 25 Aug 2019 00:08:13: #1 tags after filtering in treatment: 35671997 INFO @ Sun, 25 Aug 2019 00:08:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 25 Aug 2019 00:08:13: #1 finished! INFO @ Sun, 25 Aug 2019 00:08:13: #2 Build Peak Model... INFO @ Sun, 25 Aug 2019 00:08:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 25 Aug 2019 00:08:19: #2 number of paired peaks: 39988 INFO @ Sun, 25 Aug 2019 00:08:19: start model_add_line... INFO @ Sun, 25 Aug 2019 00:08:19: start X-correlation... INFO @ Sun, 25 Aug 2019 00:08:19: end of X-cor INFO @ Sun, 25 Aug 2019 00:08:19: #2 finished! INFO @ Sun, 25 Aug 2019 00:08:19: #2 predicted fragment length is 82 bps INFO @ Sun, 25 Aug 2019 00:08:19: #2 alternative fragment length(s) may be 82 bps INFO @ Sun, 25 Aug 2019 00:08:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20_model.r WARNING @ Sun, 25 Aug 2019 00:08:19: #2 Since the d (82) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 25 Aug 2019 00:08:19: #2 You may need to consider one of the other alternative d(s): 82 WARNING @ Sun, 25 Aug 2019 00:08:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 25 Aug 2019 00:08:19: #3 Call peaks... INFO @ Sun, 25 Aug 2019 00:08:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 25 Aug 2019 00:08:51: #3 Call peaks for each chromosome... INFO @ Sun, 25 Aug 2019 00:09:20: #3 Call peaks for each chromosome... INFO @ Sun, 25 Aug 2019 00:09:51: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05_peaks.xls INFO @ Sun, 25 Aug 2019 00:09:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05_peaks.narrowPeak INFO @ Sun, 25 Aug 2019 00:09:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.05_summits.bed INFO @ Sun, 25 Aug 2019 00:09:53: Done! pass1 - making usageList (249 chroms): 21 millis pass2 - checking and writing primary data (86230 records, 4 fields): 111 millis CompletedMACS2peakCalling INFO @ Sun, 25 Aug 2019 00:10:12: #3 Call peaks for each chromosome... INFO @ Sun, 25 Aug 2019 00:10:21: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10_peaks.xls INFO @ Sun, 25 Aug 2019 00:10:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10_peaks.narrowPeak INFO @ Sun, 25 Aug 2019 00:10:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.10_summits.bed INFO @ Sun, 25 Aug 2019 00:10:22: Done! pass1 - making usageList (171 chroms): 10 millis pass2 - checking and writing primary data (39120 records, 4 fields): 53 millis CompletedMACS2peakCalling INFO @ Sun, 25 Aug 2019 00:11:11: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20_peaks.xls INFO @ Sun, 25 Aug 2019 00:11:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20_peaks.narrowPeak INFO @ Sun, 25 Aug 2019 00:11:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX349428/SRX349428.20_summits.bed INFO @ Sun, 25 Aug 2019 00:11:11: Done! pass1 - making usageList (113 chroms): 8 millis pass2 - checking and writing primary data (17110 records, 4 fields): 26 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。