Job ID = 14171838 SRX = SRX9986164 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:56 15304469 reads; of these: 15304469 (100.00%) were unpaired; of these: 2981146 (19.48%) aligned 0 times 10269991 (67.10%) aligned exactly 1 time 2053332 (13.42%) aligned >1 times 80.52% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2496172 / 12323323 = 0.2026 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:12:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:12:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:12:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:13:02: 1000000 INFO @ Sat, 11 Dec 2021 13:13:10: 2000000 INFO @ Sat, 11 Dec 2021 13:13:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:13:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:13:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:13:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:13:26: 4000000 INFO @ Sat, 11 Dec 2021 13:13:31: 1000000 INFO @ Sat, 11 Dec 2021 13:13:34: 5000000 INFO @ Sat, 11 Dec 2021 13:13:39: 2000000 INFO @ Sat, 11 Dec 2021 13:13:43: 6000000 INFO @ Sat, 11 Dec 2021 13:13:47: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:13:51: 7000000 INFO @ Sat, 11 Dec 2021 13:13:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:13:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:13:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:13:55: 4000000 INFO @ Sat, 11 Dec 2021 13:13:59: 8000000 INFO @ Sat, 11 Dec 2021 13:14:03: 1000000 INFO @ Sat, 11 Dec 2021 13:14:04: 5000000 INFO @ Sat, 11 Dec 2021 13:14:08: 9000000 INFO @ Sat, 11 Dec 2021 13:14:12: 6000000 INFO @ Sat, 11 Dec 2021 13:14:13: 2000000 INFO @ Sat, 11 Dec 2021 13:14:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:14:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:14:15: #1 total tags in treatment: 9827151 INFO @ Sat, 11 Dec 2021 13:14:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:14:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:14:15: #1 tags after filtering in treatment: 9826997 INFO @ Sat, 11 Dec 2021 13:14:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:14:15: #1 finished! INFO @ Sat, 11 Dec 2021 13:14:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:14:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:14:16: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 13:14:16: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:14:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:14:20: 7000000 INFO @ Sat, 11 Dec 2021 13:14:22: 3000000 INFO @ Sat, 11 Dec 2021 13:14:28: 8000000 INFO @ Sat, 11 Dec 2021 13:14:32: 4000000 INFO @ Sat, 11 Dec 2021 13:14:36: 9000000 INFO @ Sat, 11 Dec 2021 13:14:41: 5000000 INFO @ Sat, 11 Dec 2021 13:14:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:14:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:14:43: #1 total tags in treatment: 9827151 INFO @ Sat, 11 Dec 2021 13:14:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:14:44: #1 tags after filtering in treatment: 9826997 INFO @ Sat, 11 Dec 2021 13:14:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:14:44: #1 finished! INFO @ Sat, 11 Dec 2021 13:14:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:14:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:14:45: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 13:14:45: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:14:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:14:50: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:14:59: 7000000 INFO @ Sat, 11 Dec 2021 13:15:09: 8000000 INFO @ Sat, 11 Dec 2021 13:15:18: 9000000 INFO @ Sat, 11 Dec 2021 13:15:25: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:15:25: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:15:25: #1 total tags in treatment: 9827151 INFO @ Sat, 11 Dec 2021 13:15:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:15:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:15:26: #1 tags after filtering in treatment: 9826997 INFO @ Sat, 11 Dec 2021 13:15:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:15:26: #1 finished! INFO @ Sat, 11 Dec 2021 13:15:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:15:26: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:15:27: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 13:15:27: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:15:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986164/SRX9986164.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling