Job ID = 14171848 SRX = SRX9986161 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:04 20907280 reads; of these: 20907280 (100.00%) were unpaired; of these: 1986495 (9.50%) aligned 0 times 14084441 (67.37%) aligned exactly 1 time 4836344 (23.13%) aligned >1 times 90.50% overall alignment rate Time searching: 00:05:04 Overall time: 00:05:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4171350 / 18920785 = 0.2205 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:16:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:16:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:16:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:17:05: 1000000 INFO @ Sat, 11 Dec 2021 13:17:12: 2000000 INFO @ Sat, 11 Dec 2021 13:17:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:17:27: 4000000 INFO @ Sat, 11 Dec 2021 13:17:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:17:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:17:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:17:34: 5000000 INFO @ Sat, 11 Dec 2021 13:17:36: 1000000 INFO @ Sat, 11 Dec 2021 13:17:43: 6000000 INFO @ Sat, 11 Dec 2021 13:17:44: 2000000 INFO @ Sat, 11 Dec 2021 13:17:51: 7000000 INFO @ Sat, 11 Dec 2021 13:17:52: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:17:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:17:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:17:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:17:59: 8000000 INFO @ Sat, 11 Dec 2021 13:18:01: 4000000 INFO @ Sat, 11 Dec 2021 13:18:05: 1000000 INFO @ Sat, 11 Dec 2021 13:18:07: 9000000 INFO @ Sat, 11 Dec 2021 13:18:09: 5000000 INFO @ Sat, 11 Dec 2021 13:18:13: 2000000 INFO @ Sat, 11 Dec 2021 13:18:15: 10000000 INFO @ Sat, 11 Dec 2021 13:18:17: 6000000 INFO @ Sat, 11 Dec 2021 13:18:22: 3000000 INFO @ Sat, 11 Dec 2021 13:18:23: 11000000 INFO @ Sat, 11 Dec 2021 13:18:25: 7000000 INFO @ Sat, 11 Dec 2021 13:18:30: 4000000 INFO @ Sat, 11 Dec 2021 13:18:32: 12000000 INFO @ Sat, 11 Dec 2021 13:18:34: 8000000 INFO @ Sat, 11 Dec 2021 13:18:39: 5000000 INFO @ Sat, 11 Dec 2021 13:18:40: 13000000 INFO @ Sat, 11 Dec 2021 13:18:42: 9000000 INFO @ Sat, 11 Dec 2021 13:18:47: 6000000 INFO @ Sat, 11 Dec 2021 13:18:48: 14000000 INFO @ Sat, 11 Dec 2021 13:18:50: 10000000 INFO @ Sat, 11 Dec 2021 13:18:54: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:18:54: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:18:54: #1 total tags in treatment: 14749435 INFO @ Sat, 11 Dec 2021 13:18:54: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:18:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:18:54: #1 tags after filtering in treatment: 14749367 INFO @ Sat, 11 Dec 2021 13:18:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:18:54: #1 finished! INFO @ Sat, 11 Dec 2021 13:18:54: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:18:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:18:55: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 13:18:55: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:18:55: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 13:18:56: 7000000 cut: /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:18:59: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:19:04: 8000000 INFO @ Sat, 11 Dec 2021 13:19:07: 12000000 INFO @ Sat, 11 Dec 2021 13:19:12: 9000000 INFO @ Sat, 11 Dec 2021 13:19:16: 13000000 INFO @ Sat, 11 Dec 2021 13:19:20: 10000000 INFO @ Sat, 11 Dec 2021 13:19:24: 14000000 INFO @ Sat, 11 Dec 2021 13:19:28: 11000000 INFO @ Sat, 11 Dec 2021 13:19:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:19:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:19:30: #1 total tags in treatment: 14749435 INFO @ Sat, 11 Dec 2021 13:19:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:19:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:19:31: #1 tags after filtering in treatment: 14749367 INFO @ Sat, 11 Dec 2021 13:19:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:19:31: #1 finished! INFO @ Sat, 11 Dec 2021 13:19:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:19:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:19:32: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 13:19:32: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:19:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:19:36: 12000000 INFO @ Sat, 11 Dec 2021 13:19:43: 13000000 INFO @ Sat, 11 Dec 2021 13:19:50: 14000000 INFO @ Sat, 11 Dec 2021 13:19:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:19:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:19:55: #1 total tags in treatment: 14749435 INFO @ Sat, 11 Dec 2021 13:19:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:19:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:19:56: #1 tags after filtering in treatment: 14749367 INFO @ Sat, 11 Dec 2021 13:19:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:19:56: #1 finished! INFO @ Sat, 11 Dec 2021 13:19:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:19:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:19:57: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 13:19:57: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:19:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986161/SRX9986161.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling