Job ID = 14171809 SRX = SRX9986154 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:54 18254559 reads; of these: 18254559 (100.00%) were unpaired; of these: 2200901 (12.06%) aligned 0 times 13215649 (72.40%) aligned exactly 1 time 2838009 (15.55%) aligned >1 times 87.94% overall alignment rate Time searching: 00:03:54 Overall time: 00:03:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3866240 / 16053658 = 0.2408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:05:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:05:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:05:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:05:08: 1000000 INFO @ Sat, 11 Dec 2021 13:05:13: 2000000 INFO @ Sat, 11 Dec 2021 13:05:18: 3000000 INFO @ Sat, 11 Dec 2021 13:05:23: 4000000 INFO @ Sat, 11 Dec 2021 13:05:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:05:32: 6000000 INFO @ Sat, 11 Dec 2021 13:05:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:05:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:05:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:05:38: 7000000 INFO @ Sat, 11 Dec 2021 13:05:40: 1000000 INFO @ Sat, 11 Dec 2021 13:05:43: 8000000 INFO @ Sat, 11 Dec 2021 13:05:46: 2000000 INFO @ Sat, 11 Dec 2021 13:05:48: 9000000 INFO @ Sat, 11 Dec 2021 13:05:52: 3000000 INFO @ Sat, 11 Dec 2021 13:05:54: 10000000 INFO @ Sat, 11 Dec 2021 13:05:58: 4000000 INFO @ Sat, 11 Dec 2021 13:05:59: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:06:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:06:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:06:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:06:04: 5000000 INFO @ Sat, 11 Dec 2021 13:06:05: 12000000 INFO @ Sat, 11 Dec 2021 13:06:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:06:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:06:06: #1 total tags in treatment: 12187418 INFO @ Sat, 11 Dec 2021 13:06:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:06:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:06:06: #1 tags after filtering in treatment: 12187290 INFO @ Sat, 11 Dec 2021 13:06:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:06:06: #1 finished! INFO @ Sat, 11 Dec 2021 13:06:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:06:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:06:07: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:06:07: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:06:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:06:10: 1000000 INFO @ Sat, 11 Dec 2021 13:06:10: 6000000 INFO @ Sat, 11 Dec 2021 13:06:16: 2000000 INFO @ Sat, 11 Dec 2021 13:06:16: 7000000 INFO @ Sat, 11 Dec 2021 13:06:22: 3000000 INFO @ Sat, 11 Dec 2021 13:06:23: 8000000 INFO @ Sat, 11 Dec 2021 13:06:28: 4000000 INFO @ Sat, 11 Dec 2021 13:06:29: 9000000 INFO @ Sat, 11 Dec 2021 13:06:35: 5000000 INFO @ Sat, 11 Dec 2021 13:06:35: 10000000 INFO @ Sat, 11 Dec 2021 13:06:41: 6000000 INFO @ Sat, 11 Dec 2021 13:06:41: 11000000 INFO @ Sat, 11 Dec 2021 13:06:47: 7000000 INFO @ Sat, 11 Dec 2021 13:06:48: 12000000 INFO @ Sat, 11 Dec 2021 13:06:49: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:06:49: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:06:49: #1 total tags in treatment: 12187418 INFO @ Sat, 11 Dec 2021 13:06:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:06:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:06:49: #1 tags after filtering in treatment: 12187290 INFO @ Sat, 11 Dec 2021 13:06:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:06:49: #1 finished! INFO @ Sat, 11 Dec 2021 13:06:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:06:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:06:50: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:06:50: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:06:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:06:53: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:07:00: 9000000 INFO @ Sat, 11 Dec 2021 13:07:06: 10000000 INFO @ Sat, 11 Dec 2021 13:07:12: 11000000 INFO @ Sat, 11 Dec 2021 13:07:18: 12000000 INFO @ Sat, 11 Dec 2021 13:07:19: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:07:19: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:07:19: #1 total tags in treatment: 12187418 INFO @ Sat, 11 Dec 2021 13:07:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:07:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:07:20: #1 tags after filtering in treatment: 12187290 INFO @ Sat, 11 Dec 2021 13:07:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:07:20: #1 finished! INFO @ Sat, 11 Dec 2021 13:07:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:07:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:07:21: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:07:21: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:07:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986154/SRX9986154.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。