Job ID = 14171810 SRX = SRX9986146 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 27367131 reads; of these: 27367131 (100.00%) were unpaired; of these: 2260990 (8.26%) aligned 0 times 13036307 (47.63%) aligned exactly 1 time 12069834 (44.10%) aligned >1 times 91.74% overall alignment rate Time searching: 00:06:48 Overall time: 00:06:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10889154 / 25106141 = 0.4337 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:09:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:09:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:09:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:09:34: 1000000 INFO @ Sat, 11 Dec 2021 13:09:38: 2000000 INFO @ Sat, 11 Dec 2021 13:09:43: 3000000 INFO @ Sat, 11 Dec 2021 13:09:48: 4000000 INFO @ Sat, 11 Dec 2021 13:09:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:09:58: 6000000 INFO @ Sat, 11 Dec 2021 13:09:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:09:59: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:09:59: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:10:03: 7000000 INFO @ Sat, 11 Dec 2021 13:10:04: 1000000 INFO @ Sat, 11 Dec 2021 13:10:08: 8000000 INFO @ Sat, 11 Dec 2021 13:10:09: 2000000 INFO @ Sat, 11 Dec 2021 13:10:13: 9000000 INFO @ Sat, 11 Dec 2021 13:10:14: 3000000 INFO @ Sat, 11 Dec 2021 13:10:18: 10000000 INFO @ Sat, 11 Dec 2021 13:10:19: 4000000 INFO @ Sat, 11 Dec 2021 13:10:23: 11000000 INFO @ Sat, 11 Dec 2021 13:10:24: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:10:28: 12000000 INFO @ Sat, 11 Dec 2021 13:10:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:10:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:10:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:10:29: 6000000 INFO @ Sat, 11 Dec 2021 13:10:33: 13000000 INFO @ Sat, 11 Dec 2021 13:10:34: 1000000 INFO @ Sat, 11 Dec 2021 13:10:34: 7000000 INFO @ Sat, 11 Dec 2021 13:10:39: 14000000 INFO @ Sat, 11 Dec 2021 13:10:39: 2000000 INFO @ Sat, 11 Dec 2021 13:10:39: 8000000 INFO @ Sat, 11 Dec 2021 13:10:40: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:10:40: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:10:40: #1 total tags in treatment: 14216987 INFO @ Sat, 11 Dec 2021 13:10:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:10:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:10:40: #1 tags after filtering in treatment: 14216893 INFO @ Sat, 11 Dec 2021 13:10:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:10:40: #1 finished! INFO @ Sat, 11 Dec 2021 13:10:40: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:10:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:10:41: #2 number of paired peaks: 1268 INFO @ Sat, 11 Dec 2021 13:10:41: start model_add_line... INFO @ Sat, 11 Dec 2021 13:10:41: start X-correlation... INFO @ Sat, 11 Dec 2021 13:10:41: end of X-cor INFO @ Sat, 11 Dec 2021 13:10:41: #2 finished! INFO @ Sat, 11 Dec 2021 13:10:41: #2 predicted fragment length is 204 bps INFO @ Sat, 11 Dec 2021 13:10:41: #2 alternative fragment length(s) may be 204 bps INFO @ Sat, 11 Dec 2021 13:10:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05_model.r INFO @ Sat, 11 Dec 2021 13:10:41: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:10:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:10:44: 3000000 INFO @ Sat, 11 Dec 2021 13:10:44: 9000000 INFO @ Sat, 11 Dec 2021 13:10:49: 4000000 INFO @ Sat, 11 Dec 2021 13:10:49: 10000000 INFO @ Sat, 11 Dec 2021 13:10:54: 5000000 INFO @ Sat, 11 Dec 2021 13:10:54: 11000000 INFO @ Sat, 11 Dec 2021 13:10:59: 6000000 INFO @ Sat, 11 Dec 2021 13:10:59: 12000000 INFO @ Sat, 11 Dec 2021 13:11:04: 7000000 INFO @ Sat, 11 Dec 2021 13:11:04: 13000000 INFO @ Sat, 11 Dec 2021 13:11:09: 8000000 INFO @ Sat, 11 Dec 2021 13:11:09: 14000000 INFO @ Sat, 11 Dec 2021 13:11:11: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:11:11: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:11:11: #1 total tags in treatment: 14216987 INFO @ Sat, 11 Dec 2021 13:11:11: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:11:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:11:11: #1 tags after filtering in treatment: 14216893 INFO @ Sat, 11 Dec 2021 13:11:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:11:11: #1 finished! INFO @ Sat, 11 Dec 2021 13:11:11: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:11:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:11:12: #2 number of paired peaks: 1268 INFO @ Sat, 11 Dec 2021 13:11:12: start model_add_line... INFO @ Sat, 11 Dec 2021 13:11:12: start X-correlation... INFO @ Sat, 11 Dec 2021 13:11:12: end of X-cor INFO @ Sat, 11 Dec 2021 13:11:12: #2 finished! INFO @ Sat, 11 Dec 2021 13:11:12: #2 predicted fragment length is 204 bps INFO @ Sat, 11 Dec 2021 13:11:12: #2 alternative fragment length(s) may be 204 bps INFO @ Sat, 11 Dec 2021 13:11:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10_model.r INFO @ Sat, 11 Dec 2021 13:11:12: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:11:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:11:13: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:11:14: 9000000 INFO @ Sat, 11 Dec 2021 13:11:19: 10000000 INFO @ Sat, 11 Dec 2021 13:11:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:11:29: 12000000 INFO @ Sat, 11 Dec 2021 13:11:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05_peaks.xls INFO @ Sat, 11 Dec 2021 13:11:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:11:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.05_summits.bed INFO @ Sat, 11 Dec 2021 13:11:29: Done! pass1 - making usageList (714 chroms): 4 millis pass2 - checking and writing primary data (27745 records, 4 fields): 40 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:11:33: 13000000 INFO @ Sat, 11 Dec 2021 13:11:38: 14000000 INFO @ Sat, 11 Dec 2021 13:11:40: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:11:40: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:11:40: #1 total tags in treatment: 14216987 INFO @ Sat, 11 Dec 2021 13:11:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:11:40: #1 tags after filtering in treatment: 14216893 INFO @ Sat, 11 Dec 2021 13:11:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:11:40: #1 finished! INFO @ Sat, 11 Dec 2021 13:11:40: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:11:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:11:41: #2 number of paired peaks: 1268 INFO @ Sat, 11 Dec 2021 13:11:41: start model_add_line... INFO @ Sat, 11 Dec 2021 13:11:41: start X-correlation... INFO @ Sat, 11 Dec 2021 13:11:41: end of X-cor INFO @ Sat, 11 Dec 2021 13:11:41: #2 finished! INFO @ Sat, 11 Dec 2021 13:11:41: #2 predicted fragment length is 204 bps INFO @ Sat, 11 Dec 2021 13:11:41: #2 alternative fragment length(s) may be 204 bps INFO @ Sat, 11 Dec 2021 13:11:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20_model.r INFO @ Sat, 11 Dec 2021 13:11:41: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:11:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:11:44: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:11:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10_peaks.xls INFO @ Sat, 11 Dec 2021 13:11:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:11:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.10_summits.bed INFO @ Sat, 11 Dec 2021 13:11:59: Done! pass1 - making usageList (632 chroms): 3 millis pass2 - checking and writing primary data (15743 records, 4 fields): 30 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:12:13: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:12:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20_peaks.xls INFO @ Sat, 11 Dec 2021 13:12:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:12:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986146/SRX9986146.20_summits.bed INFO @ Sat, 11 Dec 2021 13:12:28: Done! pass1 - making usageList (510 chroms): 2 millis pass2 - checking and writing primary data (5610 records, 4 fields): 17 millis CompletedMACS2peakCalling