Job ID = 14171777 SRX = SRX9986137 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:55 34658013 reads; of these: 34658013 (100.00%) were unpaired; of these: 4991272 (14.40%) aligned 0 times 6732101 (19.42%) aligned exactly 1 time 22934640 (66.17%) aligned >1 times 85.60% overall alignment rate Time searching: 00:07:55 Overall time: 00:07:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 22180721 / 29666741 = 0.7477 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:00:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:00:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:00:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:00:20: 1000000 INFO @ Sat, 11 Dec 2021 13:00:26: 2000000 INFO @ Sat, 11 Dec 2021 13:00:31: 3000000 INFO @ Sat, 11 Dec 2021 13:00:36: 4000000 INFO @ Sat, 11 Dec 2021 13:00:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:00:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:00:45: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:00:45: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:00:47: 6000000 INFO @ Sat, 11 Dec 2021 13:00:52: 1000000 INFO @ Sat, 11 Dec 2021 13:00:54: 7000000 INFO @ Sat, 11 Dec 2021 13:00:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:00:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:00:57: #1 total tags in treatment: 7486020 INFO @ Sat, 11 Dec 2021 13:00:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:00:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:00:57: #1 tags after filtering in treatment: 7485917 INFO @ Sat, 11 Dec 2021 13:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:00:57: #1 finished! INFO @ Sat, 11 Dec 2021 13:00:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:00:58: #2 number of paired peaks: 1716 INFO @ Sat, 11 Dec 2021 13:00:58: start model_add_line... INFO @ Sat, 11 Dec 2021 13:00:58: start X-correlation... INFO @ Sat, 11 Dec 2021 13:00:58: end of X-cor INFO @ Sat, 11 Dec 2021 13:00:58: #2 finished! INFO @ Sat, 11 Dec 2021 13:00:58: #2 predicted fragment length is 193 bps INFO @ Sat, 11 Dec 2021 13:00:58: #2 alternative fragment length(s) may be 193 bps INFO @ Sat, 11 Dec 2021 13:00:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05_model.r INFO @ Sat, 11 Dec 2021 13:00:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:00:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:00:59: 2000000 INFO @ Sat, 11 Dec 2021 13:01:05: 3000000 INFO @ Sat, 11 Dec 2021 13:01:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:01:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:01:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:01:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:01:15: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:01:19: 5000000 INFO @ Sat, 11 Dec 2021 13:01:22: 1000000 INFO @ Sat, 11 Dec 2021 13:01:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05_peaks.xls INFO @ Sat, 11 Dec 2021 13:01:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:01:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.05_summits.bed INFO @ Sat, 11 Dec 2021 13:01:24: Done! pass1 - making usageList (622 chroms): 2 millis pass2 - checking and writing primary data (5170 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:01:26: 6000000 INFO @ Sat, 11 Dec 2021 13:01:29: 2000000 INFO @ Sat, 11 Dec 2021 13:01:33: 7000000 INFO @ Sat, 11 Dec 2021 13:01:36: 3000000 INFO @ Sat, 11 Dec 2021 13:01:37: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:01:37: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:01:37: #1 total tags in treatment: 7486020 INFO @ Sat, 11 Dec 2021 13:01:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:01:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:01:37: #1 tags after filtering in treatment: 7485917 INFO @ Sat, 11 Dec 2021 13:01:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:01:37: #1 finished! INFO @ Sat, 11 Dec 2021 13:01:37: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:01:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:01:38: #2 number of paired peaks: 1716 INFO @ Sat, 11 Dec 2021 13:01:38: start model_add_line... INFO @ Sat, 11 Dec 2021 13:01:38: start X-correlation... INFO @ Sat, 11 Dec 2021 13:01:38: end of X-cor INFO @ Sat, 11 Dec 2021 13:01:38: #2 finished! INFO @ Sat, 11 Dec 2021 13:01:38: #2 predicted fragment length is 193 bps INFO @ Sat, 11 Dec 2021 13:01:38: #2 alternative fragment length(s) may be 193 bps INFO @ Sat, 11 Dec 2021 13:01:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10_model.r INFO @ Sat, 11 Dec 2021 13:01:38: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:01:38: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:01:43: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:01:50: 5000000 INFO @ Sat, 11 Dec 2021 13:01:55: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:01:57: 6000000 INFO @ Sat, 11 Dec 2021 13:02:03: 7000000 INFO @ Sat, 11 Dec 2021 13:02:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10_peaks.xls INFO @ Sat, 11 Dec 2021 13:02:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:02:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.10_summits.bed INFO @ Sat, 11 Dec 2021 13:02:03: Done! pass1 - making usageList (542 chroms): 1 millis pass2 - checking and writing primary data (3017 records, 4 fields): 16 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:02:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:02:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:02:06: #1 total tags in treatment: 7486020 INFO @ Sat, 11 Dec 2021 13:02:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:02:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:02:07: #1 tags after filtering in treatment: 7485917 INFO @ Sat, 11 Dec 2021 13:02:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 13:02:07: #1 finished! INFO @ Sat, 11 Dec 2021 13:02:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:02:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:02:07: #2 number of paired peaks: 1716 INFO @ Sat, 11 Dec 2021 13:02:07: start model_add_line... INFO @ Sat, 11 Dec 2021 13:02:08: start X-correlation... INFO @ Sat, 11 Dec 2021 13:02:08: end of X-cor INFO @ Sat, 11 Dec 2021 13:02:08: #2 finished! INFO @ Sat, 11 Dec 2021 13:02:08: #2 predicted fragment length is 193 bps INFO @ Sat, 11 Dec 2021 13:02:08: #2 alternative fragment length(s) may be 193 bps INFO @ Sat, 11 Dec 2021 13:02:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20_model.r INFO @ Sat, 11 Dec 2021 13:02:08: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:02:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:02:25: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:02:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20_peaks.xls INFO @ Sat, 11 Dec 2021 13:02:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:02:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9986137/SRX9986137.20_summits.bed INFO @ Sat, 11 Dec 2021 13:02:34: Done! pass1 - making usageList (432 chroms): 1 millis pass2 - checking and writing primary data (1669 records, 4 fields): 13 millis CompletedMACS2peakCalling