Job ID = 14171717 SRX = SRX9986123 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:03 22823812 reads; of these: 22823812 (100.00%) were unpaired; of these: 2479414 (10.86%) aligned 0 times 16890362 (74.00%) aligned exactly 1 time 3454036 (15.13%) aligned >1 times 89.14% overall alignment rate Time searching: 00:05:03 Overall time: 00:05:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5325431 / 20344398 = 0.2618 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:43:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:43:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:43:49: 1000000 INFO @ Sat, 11 Dec 2021 12:43:53: 2000000 INFO @ Sat, 11 Dec 2021 12:43:58: 3000000 INFO @ Sat, 11 Dec 2021 12:44:03: 4000000 INFO @ Sat, 11 Dec 2021 12:44:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:44:12: 6000000 INFO @ Sat, 11 Dec 2021 12:44:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:44:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:44:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:44:17: 7000000 INFO @ Sat, 11 Dec 2021 12:44:20: 1000000 INFO @ Sat, 11 Dec 2021 12:44:22: 8000000 INFO @ Sat, 11 Dec 2021 12:44:25: 2000000 INFO @ Sat, 11 Dec 2021 12:44:27: 9000000 INFO @ Sat, 11 Dec 2021 12:44:30: 3000000 INFO @ Sat, 11 Dec 2021 12:44:32: 10000000 INFO @ Sat, 11 Dec 2021 12:44:35: 4000000 INFO @ Sat, 11 Dec 2021 12:44:37: 11000000 INFO @ Sat, 11 Dec 2021 12:44:40: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:44:42: 12000000 INFO @ Sat, 11 Dec 2021 12:44:45: 6000000 INFO @ Sat, 11 Dec 2021 12:44:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:44:45: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:44:45: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:44:47: 13000000 INFO @ Sat, 11 Dec 2021 12:44:50: 7000000 INFO @ Sat, 11 Dec 2021 12:44:50: 1000000 INFO @ Sat, 11 Dec 2021 12:44:52: 14000000 INFO @ Sat, 11 Dec 2021 12:44:55: 8000000 INFO @ Sat, 11 Dec 2021 12:44:55: 2000000 INFO @ Sat, 11 Dec 2021 12:44:57: 15000000 INFO @ Sat, 11 Dec 2021 12:44:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:44:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:44:57: #1 total tags in treatment: 15018967 INFO @ Sat, 11 Dec 2021 12:44:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:44:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:44:58: #1 tags after filtering in treatment: 15018869 INFO @ Sat, 11 Dec 2021 12:44:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:44:58: #1 finished! INFO @ Sat, 11 Dec 2021 12:44:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:44:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:44:59: #2 number of paired peaks: 60 WARNING @ Sat, 11 Dec 2021 12:44:59: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:44:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:45:00: 9000000 INFO @ Sat, 11 Dec 2021 12:45:00: 3000000 INFO @ Sat, 11 Dec 2021 12:45:05: 10000000 INFO @ Sat, 11 Dec 2021 12:45:05: 4000000 INFO @ Sat, 11 Dec 2021 12:45:10: 11000000 INFO @ Sat, 11 Dec 2021 12:45:10: 5000000 INFO @ Sat, 11 Dec 2021 12:45:15: 12000000 INFO @ Sat, 11 Dec 2021 12:45:15: 6000000 INFO @ Sat, 11 Dec 2021 12:45:20: 13000000 INFO @ Sat, 11 Dec 2021 12:45:20: 7000000 INFO @ Sat, 11 Dec 2021 12:45:24: 14000000 INFO @ Sat, 11 Dec 2021 12:45:25: 8000000 INFO @ Sat, 11 Dec 2021 12:45:29: 15000000 INFO @ Sat, 11 Dec 2021 12:45:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:45:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:45:30: #1 total tags in treatment: 15018967 INFO @ Sat, 11 Dec 2021 12:45:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:45:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:45:30: 9000000 INFO @ Sat, 11 Dec 2021 12:45:30: #1 tags after filtering in treatment: 15018869 INFO @ Sat, 11 Dec 2021 12:45:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:45:30: #1 finished! INFO @ Sat, 11 Dec 2021 12:45:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:45:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:45:31: #2 number of paired peaks: 60 WARNING @ Sat, 11 Dec 2021 12:45:31: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:45:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:45:34: 10000000 INFO @ Sat, 11 Dec 2021 12:45:39: 11000000 INFO @ Sat, 11 Dec 2021 12:45:44: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:45:49: 13000000 INFO @ Sat, 11 Dec 2021 12:45:53: 14000000 INFO @ Sat, 11 Dec 2021 12:45:58: 15000000 INFO @ Sat, 11 Dec 2021 12:45:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:45:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:45:58: #1 total tags in treatment: 15018967 INFO @ Sat, 11 Dec 2021 12:45:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:45:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:45:59: #1 tags after filtering in treatment: 15018869 INFO @ Sat, 11 Dec 2021 12:45:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:45:59: #1 finished! INFO @ Sat, 11 Dec 2021 12:45:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:45:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:46:00: #2 number of paired peaks: 60 WARNING @ Sat, 11 Dec 2021 12:46:00: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:46:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986123/SRX9986123.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。