Job ID = 14171703 SRX = SRX9986122 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:59 13023555 reads; of these: 13023555 (100.00%) were unpaired; of these: 1745341 (13.40%) aligned 0 times 9207932 (70.70%) aligned exactly 1 time 2070282 (15.90%) aligned >1 times 86.60% overall alignment rate Time searching: 00:02:59 Overall time: 00:02:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1868257 / 11278214 = 0.1657 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:37:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:37:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:37:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:37:48: 1000000 INFO @ Sat, 11 Dec 2021 12:37:55: 2000000 INFO @ Sat, 11 Dec 2021 12:38:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:38:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:38:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:38:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:38:10: 4000000 INFO @ Sat, 11 Dec 2021 12:38:18: 1000000 INFO @ Sat, 11 Dec 2021 12:38:18: 5000000 INFO @ Sat, 11 Dec 2021 12:38:25: 2000000 INFO @ Sat, 11 Dec 2021 12:38:26: 6000000 INFO @ Sat, 11 Dec 2021 12:38:33: 3000000 INFO @ Sat, 11 Dec 2021 12:38:34: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:38:40: 4000000 INFO @ Sat, 11 Dec 2021 12:38:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:38:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:38:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:38:43: 8000000 INFO @ Sat, 11 Dec 2021 12:38:47: 1000000 INFO @ Sat, 11 Dec 2021 12:38:48: 5000000 INFO @ Sat, 11 Dec 2021 12:38:50: 9000000 INFO @ Sat, 11 Dec 2021 12:38:53: 2000000 INFO @ Sat, 11 Dec 2021 12:38:54: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:38:54: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:38:54: #1 total tags in treatment: 9409957 INFO @ Sat, 11 Dec 2021 12:38:54: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:38:55: #1 tags after filtering in treatment: 9409830 INFO @ Sat, 11 Dec 2021 12:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:38:55: #1 finished! INFO @ Sat, 11 Dec 2021 12:38:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:38:55: 6000000 INFO @ Sat, 11 Dec 2021 12:38:55: #2 number of paired peaks: 80 WARNING @ Sat, 11 Dec 2021 12:38:55: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:38:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:38:59: 3000000 INFO @ Sat, 11 Dec 2021 12:39:03: 7000000 INFO @ Sat, 11 Dec 2021 12:39:05: 4000000 INFO @ Sat, 11 Dec 2021 12:39:11: 8000000 INFO @ Sat, 11 Dec 2021 12:39:11: 5000000 INFO @ Sat, 11 Dec 2021 12:39:18: 6000000 INFO @ Sat, 11 Dec 2021 12:39:18: 9000000 INFO @ Sat, 11 Dec 2021 12:39:21: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:39:21: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:39:21: #1 total tags in treatment: 9409957 INFO @ Sat, 11 Dec 2021 12:39:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:39:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:39:22: #1 tags after filtering in treatment: 9409830 INFO @ Sat, 11 Dec 2021 12:39:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:39:22: #1 finished! INFO @ Sat, 11 Dec 2021 12:39:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:39:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:39:23: #2 number of paired peaks: 80 WARNING @ Sat, 11 Dec 2021 12:39:23: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:39:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:39:24: 7000000 INFO @ Sat, 11 Dec 2021 12:39:31: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:39:37: 9000000 INFO @ Sat, 11 Dec 2021 12:39:40: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:39:40: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:39:40: #1 total tags in treatment: 9409957 INFO @ Sat, 11 Dec 2021 12:39:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:39:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:39:41: #1 tags after filtering in treatment: 9409830 INFO @ Sat, 11 Dec 2021 12:39:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:39:41: #1 finished! INFO @ Sat, 11 Dec 2021 12:39:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:39:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:39:41: #2 number of paired peaks: 80 WARNING @ Sat, 11 Dec 2021 12:39:41: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:39:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986122/SRX9986122.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。