Job ID = 14171674 SRX = SRX9986119 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:28 16487570 reads; of these: 16487570 (100.00%) were unpaired; of these: 1981482 (12.02%) aligned 0 times 11916045 (72.27%) aligned exactly 1 time 2590043 (15.71%) aligned >1 times 87.98% overall alignment rate Time searching: 00:03:28 Overall time: 00:03:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3028031 / 14506088 = 0.2087 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:30:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:30:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:30:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:30:24: 1000000 INFO @ Sat, 11 Dec 2021 12:30:29: 2000000 INFO @ Sat, 11 Dec 2021 12:30:34: 3000000 INFO @ Sat, 11 Dec 2021 12:30:39: 4000000 INFO @ Sat, 11 Dec 2021 12:30:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:30:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:30:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:30:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:30:50: 6000000 INFO @ Sat, 11 Dec 2021 12:30:54: 1000000 INFO @ Sat, 11 Dec 2021 12:30:55: 7000000 INFO @ Sat, 11 Dec 2021 12:31:00: 2000000 INFO @ Sat, 11 Dec 2021 12:31:01: 8000000 INFO @ Sat, 11 Dec 2021 12:31:06: 3000000 INFO @ Sat, 11 Dec 2021 12:31:06: 9000000 INFO @ Sat, 11 Dec 2021 12:31:11: 4000000 INFO @ Sat, 11 Dec 2021 12:31:12: 10000000 INFO @ Sat, 11 Dec 2021 12:31:17: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:31:17: 11000000 INFO @ Sat, 11 Dec 2021 12:31:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:31:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:31:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:31:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:31:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:31:20: #1 total tags in treatment: 11478057 INFO @ Sat, 11 Dec 2021 12:31:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:31:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:31:21: #1 tags after filtering in treatment: 11477937 INFO @ Sat, 11 Dec 2021 12:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:31:21: #1 finished! INFO @ Sat, 11 Dec 2021 12:31:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:31:21: #2 number of paired peaks: 59 WARNING @ Sat, 11 Dec 2021 12:31:21: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:31:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:31:22: 6000000 INFO @ Sat, 11 Dec 2021 12:31:25: 1000000 INFO @ Sat, 11 Dec 2021 12:31:28: 7000000 INFO @ Sat, 11 Dec 2021 12:31:30: 2000000 INFO @ Sat, 11 Dec 2021 12:31:33: 8000000 INFO @ Sat, 11 Dec 2021 12:31:36: 3000000 INFO @ Sat, 11 Dec 2021 12:31:39: 9000000 INFO @ Sat, 11 Dec 2021 12:31:41: 4000000 INFO @ Sat, 11 Dec 2021 12:31:45: 10000000 INFO @ Sat, 11 Dec 2021 12:31:47: 5000000 INFO @ Sat, 11 Dec 2021 12:31:50: 11000000 INFO @ Sat, 11 Dec 2021 12:31:53: 6000000 INFO @ Sat, 11 Dec 2021 12:31:53: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:31:53: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:31:53: #1 total tags in treatment: 11478057 INFO @ Sat, 11 Dec 2021 12:31:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:31:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:31:54: #1 tags after filtering in treatment: 11477937 INFO @ Sat, 11 Dec 2021 12:31:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:31:54: #1 finished! INFO @ Sat, 11 Dec 2021 12:31:54: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:31:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:31:54: #2 number of paired peaks: 59 WARNING @ Sat, 11 Dec 2021 12:31:54: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:31:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:31:58: 7000000 INFO @ Sat, 11 Dec 2021 12:32:03: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:32:08: 9000000 INFO @ Sat, 11 Dec 2021 12:32:14: 10000000 INFO @ Sat, 11 Dec 2021 12:32:20: 11000000 INFO @ Sat, 11 Dec 2021 12:32:23: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:32:23: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:32:23: #1 total tags in treatment: 11478057 INFO @ Sat, 11 Dec 2021 12:32:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:32:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:32:23: #1 tags after filtering in treatment: 11477937 INFO @ Sat, 11 Dec 2021 12:32:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:32:23: #1 finished! INFO @ Sat, 11 Dec 2021 12:32:23: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:32:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:32:24: #2 number of paired peaks: 59 WARNING @ Sat, 11 Dec 2021 12:32:24: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:32:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986119/SRX9986119.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。